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CSII-EF-RfA-IRES-Puro

Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.

Catalog number RDB12869
Resource name CSII-EF-RfA-IRES-Puro
Clone info. Backbone vector plasmid of lentivirus construct driven by EF-1 alpha promoter by Gateway(R) cloning with puromycin resistance marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-RfA-IRES-Puro (Plasmid)
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB12869zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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            提供依頼書 [Word]
            提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

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            RDB12869 CSII-EF-RfA-IRES-Puro DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-EF-RfA-IRES-Puro was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12869).

            Reference section:

            Kurita, R., Suda, N., Sudo, K., Miharada, K., Hiroyama, T., Miyoshi, H., Tani, K., Nakamura, Y., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12869_A9I3p1-3.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1a pro, attR1, lac UV5 pro, CmR 5'
            Sequence file: RDB12869_A9I3a.seq check
            >12869_12869_A9I3_1_EF1a-C_F_A07_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGAGTTTGG NNCTTGGTTC NTTCTCNAGC CTCAGACAGT
               61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CTCGAGAATT
              121 ATCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA
              181 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG
              241 TCACTATGGC GGCCGCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATA
              301 ATGTGTGGAT TTTGAGTTAG GATCCGTCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG
              361 AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT
              421 TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA
              481 CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA
              541 TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTCCGTAT GGCAATGAAA GACGGTGAGC
              601 TGGTGATATG GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT
              661 TTTCATCGCT CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC
              721 AAGATGTGNC GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGNTT ATTGAGAATA
              781 TGTTTTTCGT CTCAGCCNAT CNCTGGGTGA GTTTCACCAG TTTTGATTTA ANCGTGGCCA
              841 ATATGGACAA CTTCTTCGCC CCCGTTTTCA CCANGGGCAA ATATTATACG CAAGGCGNNA
              901 ANGNGCTGAT GNCGCNGGCG ANTCNNGTNC ATCATGCCGT TNGTGATGGC TNCNNTGNNG
              961 NNANANNGCN TNNNNGANTT ACAACAGNAC TGNNANNANN GNNANGNNNG GGNNNNNANN
            1021 NNNNNNNN
            //
            Primer: Cm_C_F (Pr0476)
            Region: ccdB, attR2
            Sequence file: RDB12869_A9I3b.seq check
            >12869_12869_A9I3_1_Cm_C_F_B07_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGNTTNNNNN NNNCNNGANA ACNGTATGCG TATTTGCGCG
               61 CTGATTTTTG CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG TCAAAAAGAG
              121 GTATGCTATG AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC AGTTGCTCAA
              181 GGCATATATG ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA TGAAGCCCGT
              241 CGTCTGCGTG CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA GGTCGCCCGG
              301 TTTATTGAAA TGAACGGCTC TTTTGCTGAC GAGAACAGGG GCTGGTGAAA TGCAGTTTAA
              361 GGTTTACACC TATAAAAGAG AGAGCCGTTA TCGTCTGTTT GTGGATGTAC AGAGTGATAT
              421 TATTGACACG CCCGGGCGAC GGATGGTGAT CCCCCTGGCC AGTGCACGTC TGCTGTCAGA
              481 TAAAGTCTCC CGTGAACTTT ACCCGGTGGT GCATATCGGG GATGAAAGCT GGCGCATGAT
              541 GACCACCGAT ATGGCCAGTG TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG
              601 CCACCGCGAA AATGACATCA AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC
              661 AGGCTCCCTT ATACACAGCC AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT
              721 TACAGTATTA TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT
              781 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACNAAGTGGT GATAATTCTG CAGCGGCCGC
              841 GGATCCTCTA NACTGGANTT AATTCGCTGT CTGCGANGGN NAGCTGTTGG GGTGAGTACT
              901 CCNNCTCAAA ANCGGGCATG ANTTCTGCGC TNNNANTGNC AGTTTCCNAA ANCGANGANG
              961 ANTTGATATN CACNNNGCCN NNNGNGATGN NNTTGNNGNT GGNNNCNNCC ATCTGGNCAN
            1021 AAAANNNNNN TTTTNNNNNN NNNN
            //
            Primer: IRES-F (Pr0036)
            Region: IRES, PuroR
            Sequence file: RDB12869_A9I3c.seq check
            >12869_12869_A9I3_1_IRES-F_C07_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNTNNGT CGANGNNNNN NAACGTCTAG GCCCCCCGAA CCNCGGGGNC
               61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA GCTTGCCACA ACCCACAAGG AGACGACCTT
              121 CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG
              181 TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG
              241 ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG
              301 ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG
              361 AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG
              421 GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG
              481 AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG
              541 GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC
              601 TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG
              661 CCGACGTCGA NGTGCCCGAA NGANCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT
              721 GAAACATCGA GGGATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
              781 ANTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGANACG CTGCTTTAAT
              841 GNCTTTGTAT CATGCTATTG CTTCCCGNAT GGNTTTCATT TTCTCCTCCT NGTATAAATC
              901 CTGGTTGCTG TCTCTTTATG ANGANTTGTG GCCNGTTGTC NNNNAACGNG GCGNNNNNNG
              961 CACNGNNTTN NNTGNNGCNN NCCCCNNCNN NNTNNGGGGC ATTGNNNCCN NCNGNCANNN
            1021 NNNTTNNNNG GNNNNTNNNN TTNNNNNNNN NCNNNNNNNN NN
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, 5'LTR, HIV-1psi
            Sequence file: RDB12869_A9I3d.seq check
            >12869_12869_A9I3_1_CMV_Forward_D07_10_ABI24.ab1
                1 NNNNNNNNNN NNNNCNNNNN NNCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
               61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
              121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
              181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
              241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
              301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
              361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
              421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
              481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
              541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
              601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
              661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
              721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG
              781 ATCTTCAGAC CTGGANGANG AGANATGANG GANAATTGGA GAAGTGAATT ATATAAATAT
              841 AAAGTAGTAA AAATTGAACC NTTANGAGTA GCACCCACCA NNGCAAAGAG AAGANTGGNG
              901 CAGANAGAAA AAAAGAGCAN NGGGAATAGG NNCTTTGTTC CNNNGNNTCT TGGNNNCAGC
            961 NNNAAGCNCT NNNGGCGCNN CNNNNNTGNN NNNNNNNGNN NNNNNNNN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGH p(A), 3'LTR, PRE
            Sequence file: RDB12869_A9I3e.seq check
            >12869_12869_A9I3_1_BGH_rev2_E07_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
               61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
              121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
              181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
              241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
              301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
              361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
              421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
              481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
              541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
              601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
              661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
              721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC
              781 GACGGTATCG ATGCGGGGAN GCGGNCCAAA GGGAGATCCG ANTCGTCTGA NGGCGAANGC
              841 GAAGANGCGG AANANGNNGC ANANCCNGCA GCANGNCGCG GGAANGAANG TCCGCTGGNN
              901 TGANGGNCGA ANGGNNGTAN CANANNGNNN TCCCGCGCAN AATCCANGNG GNNACNCNGG
            961 NNANCANNNN TGGNAAGGNN GTCNNCTTNC NNNNNNNNNN
            //
            Primer: SV40pro_F (Pr0110)
            Region: SV40 pro_ori, EM7 pro, Zeo, SV40 p(A)
            Sequence file: RDB12869_A9I3f.seq check
            >12869_12869_A9I3_1_SV40pro_F_F07_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNANNAGTGA GGNNGNTTTT TTGGAGGCCT AGGCTTTTGC
               61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT
              121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG
              181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT
              241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG
              301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA
              361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG
              421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT
              481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG
              541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
              601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
              661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
              721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
              781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GNNCTCTAGC TAGAGCTTGN
              841 CGTAATCATG GNCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTNCACACA
              901 ACATACGANC NNGAAGCATA AAGTGTAAAG CCNGGGGNGN CNNNTGAGTG ANCNAACNCN
              961 CATTAATTGN GTTGCNCNCA CNGNCNNNNT TNNAGNCNGN NAANNNNNNN NNGNCNNNNN
            1021 NNNNN
            //
            Primer: cPPT_CTS-E_R (Pr0725)
            Region: cPPT/CTS, RRE
            Sequence file: RDB12869_A9I3g.seq check
            >12869_12869_A9I3_1_CPPT_CTS-E_R_G06_21_ABI24.ab1
                1 NNNNNNNNNN TNGNTTTTGN NNTTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
               61 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
              121 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
              181 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
              241 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
              301 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
              361 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
              421 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
              481 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA
              541 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC
              601 CAAATCCCCA GGAGCTGTTG ATCCTTTAGG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG
              661 AGCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC
              721 AGCAAATTGT TCTGCTGCTG CACTATACCA GACAATAATT GTCTGGCCTG TACCGTCAGC
              781 GTCATTGACG CTGCGCCCAT AGTGCTTCCT GCTGCTCCCN ANAACCCAAG GAACAAAGCT
              841 CCNATTCCCA CTNCNCTTTT TTTCTCTCTG CACCACTCTT CTCTTNGCCT NGGNGGGNGC
              901 TACNNCNAAN NGTTCAATTT TTACNACTTT ATATTTATAT NATTNNCTTC NCCNATTNNC
            961 CCNNATATCT NNNNNNNNNN
            //
            Primer: ccdb-M_F (Pr0951)
            Region: ccdB, attR2,chimeric intron,IRES
            Sequence file: RDB12869_A9I3h.seq check
            >12869_12869_B1LkF_ccdb-M_F_C05_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGNNNNANNG GCTGNNCTNN GCNNCCGCGA AAATGACATC
               61 NAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC
              121 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG
              181 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC
              241 AGCTTTCTTG TACAAAGTGG TGATAATTCT GCAGCGGCCG CGGATCCTCT AGACTGGAAT
              301 TAATTCGCTG TCTGCGAGGG CCAGCTGTTG GGGTGAGTAC TCCCTCTCAA AAGCGGGCAT
              361 GACTTCTGCG CTAAGATTGT CAGTTTCCAA AAACGAGGAG GATTTGATAT TCACCTGGCC
              421 CGCGGTGATG CCTTTGAGGG TGGCCGCGTC CATCTGGTCA GAAAAGACAA TCTTTTTGTT
              481 GTCAAGCTTG AGGTGTGGCA GGCTTGGGAT CTGGCCATAC ACTTGAGTGA CAATGACATC
              541 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGGTCC AACTGCAGGT CGAGCATGCA
              601 TCTAGGGCGG CCAATTCCGC CCCTCTCCCC NNNNNNCCTC TCCCTCCCCC CCCCCTAACG
              661 TTACTGGCCG AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA
              721 CCNTATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAANC TGGCCCTGTC TTCTTGACNA
              781 NNNNTNNTAN GNNNNNNTTC NNCTCTCGCN NAAGGNATGC NNNNGNNNNN NGAANGTCNT
              841 GAANGAAGCA NNTCNTCTNG NANCTTCNNN NNNANAAACA NCGTNNGTNN NGANCNNTTN
              901 NNNNGCNNNN NAACCCCCCC ANCNGGNCGN NNNGNNNCNT CNGCNNNNNN AAGNNACNNN
              961 GTANNAANAN NNNNCCTGNN AANNNNGGNA CNANCCCCNN TGNCNNNGTN GTNANNTNGG
             1021 ANNANTTNNN GNNAANANNC NANNNGNNNN NCNNNNNGNN NNNNTNNNNC NNNGGGNNNN
            1081 ANNNNNNNNC NNNNNNNNCC CNNNTNNNNN NNGNNNNNNN NNN
            //
            Primer: PuroR-rev (Pr0423)
            Region: IRES
            Sequence file: RDB12869_A9I3i.seq check
            >12869_12869_B1LkF_PuroR-rev_E05_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGGNNN NNNNNNNGCT TATCNTCGTG TTTTTCNAAG
               61 GAAAACCACG TCCCCGTGGT TCGGGGGGCC TAGACGTTTT TTTAACCTCG ACTAAACACA
              121 TGTAAAGCAT GTGCACCGAG GCCCCAGATC AGATCCCATA CAATGGGGTA CCTTCTGGGC
              181 ATCCTTCAGC CCCTTGTTGA ATACGCTTGA GGAGAGCCAT TTGACTCTTT CCACAACTAT
              241 CCAACTCACA ACGTGGCACT GGGGTTGTGC CGCCTTTGCA GGTGTATCTT ATACACGTGG
              301 CTTTTGGCCG CAGAGGCACC TGTCGCCAGG TGGGGGGTTC CGCTGCCTGC AAAGGGTCGC
              361 TACAGACGTT GTTTGTCTTC AAGAAGCTTC CAGAGGAACT GCTTCCTTCA CGACATTCAA
              421 CAGACCTTGC ATTCCTTTGG CGAGAGGGGA AAGACCCCTA GGAATGCTCG TCAAGAAGAC
              481 AGGGCCAGGT TTCCGGGCCC TCACATTGCC AAAAGACGGC AATATGGTGG AAAATAACAT
              541 ATAGACAAAC GCACACCGGC CTTATTCCAA GCGGCTTCGG CCAGTAACGT TAGGGGGGGG
            601 GGAGGGAGAG GGGGGGGN
            //
            Primer: mCherry-check_R5 (Pr1024)
            Region: PuroR
            Sequence file: RDB12869_A9I3j.seq check
            >12869_12869_B1LkF_mCherry-check_R5_F05_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGTAAAANG ANNNNACATA
               61 NNTNANAATA CCAGTCNATC TTTCNCAAAT TTTGTAATCC AGAGGTTGAT TATCGAATAA
              121 GCTTGATCCC TCGATGTTTC AGGCACCGGG CTTGCGGGTC ATGCACCAGG TGCGCGGTCC
              181 TTCGGGCACC TCGACGTCGG CGGTGACGGT GAAGCCGAGC CGCTCGTAGA AGGGGAGGTT
              241 GCGGGGCGCG GAGGTCTCCA GGAAGGCGGG CACCCCGGCG CGCTCGGCCG CCTCCACTCC
              301 GGGGAGCACG ACGGCGCTGC CCAGACCCTT GCCCTGGTGG TCGGGCGAGA CGCCGACGGT
              361 GGCCAGGAAC CACGCGGGCT CCTTGGGCCG GTGCGGCGCC AGGAGGCCTT CCATCTGTTG
              421 CTGCGCGGCC AGCCGGGAAC CGCTCAACTC GGCCATGCGC GGGCCGATCT CGGCGAACAC
              481 CGCCCCCGCT TCGACGCTCT CCGGCGTGGT CCAGACCGCC ACCGCGGCGC CGTCGTCCGC
              541 GACCCACACC TTGCCGATGT CGAGCCCGAC GCGCGTGAGG AAGAGTTCTT GCAGCTCGGT
              601 GACCCGCTCG ATGTGGCGGT CCGGGTCGAC GGTGTGGCGC GTGGCGGGGT AGTCGGCGAA
              661 CGCGGCGGCG AGGGTGCGTA CGGCCCGGGG GNANGTCGTC GNNNNNGGCG AGGCGCACCG
              721 TGGGCTTGTA CTCGGTCATG GAANNGNNNN CTCCTTGTGG GTTGTGNNAA GCTTATCATC
              781 GNGNTTTTCA AAGGNAAAAC CNNNGTCCCC GTGGNTCGGG GGGGCCTANA CGTTTTTTTA
              841 ACCTCNNCTA ANCACNNTGN NAAAGCANGT GCACCGANGC CCNNNATCAG ATNCCATACA
              901 ATGGGGNTAC CTTCTGGGNC NTCCTTCANC CCCNTNGTNN AANNNNNNTN NAGNANANNC
              961 NNNTTTGACT CTTNNNNNNN ACTATNCNNC NNNNNANNGT GGNNCTGGGG TTGTGNCGCC
             1021 TTTNNNNNNN NANCNTANNC NCGNNNNNTT TTGNNNGNNN ANGNNNNNNG NNNGNNNNGT
            1081 NNNGGGGNTN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP]
            user_report Onuma, S., The Lack of Bmal1, a Core Clock Gene, in the Intestine Decreases Glucose Absorption in Mice. Endocrinology 163 (9): bqac119 (2022). PMID 35904419. [link to RRC of NBRP]
            user_report Kawai, M., Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels. Sci. Rep. 10 (1): 21501 (2020). PMID 33299044. [link to RRC of NBRP]
            user_report Hashizume, O., Epigenetic regulation of the nuclear-coded GCAT and SHMT2 genes confers human age-associated mitochondrial respiration defects. Sci. Rep. 5: 10434 (2015). PMID 26000717. [link to RRC of NBRP]

            2024.03.26

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