Please review the QC test results indicated by icon below as well as clone information before placing your order.

GFP-NFATc4(FL)/pCAGEN

Expression clone of mouse Nfatc4 tagged with GFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Genes Dev., 27, 2708-2721 (2013)) Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Genes Dev., 27, 2708-2721 (2013)) 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12860_A4Iop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C Sequence file: RDB12860_A4Ioa.seq
>12860_12860_A4Io_EGFP-C_G11_20.ab1     1 TCAGGGGACA TCTTCGGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTAT    61 GGGGGCCGCA AGCTGCGAGG ATGAGGAGCT GGAATTTAAG CTGGTGTTCG GAGAGGAAAA   121 GGAGCCCCCC CCGCTGGGCC CTGGGGGTCC TGGGGAAGAG CTGGACTCAG AGGATACCCC   181 TCCATGCTGT CGTTTGGCCC TGGGGGAACC CCTTCCTTAT GGTGCTGCCC CTATTGGGAT   241 TCCCCGACCC CCACCCCCTC GGCCAGGGAT GCACTCACCA CCGCCCCGTC CCGCCCCTTC   301 ACCTGGCACC TGGGAGAGCC AGCCAGCTAG GTCGGTGAGG CTGGGGGGCC CAGGAGGTAA   361 TGCAGGGGGT GCTGGGGGTG GTCGTGTTCT TGAGTGTCCC AGCATCCGGA TTACCTCCAT   421 CTCTCCCACG CCTGACCCGC CGACCTCGCT AGAGGACACA TCTGAAACTT GGGGGGACGG   481 CTCTCCTAGA GATTACCCTC CACCAGAAGG CTTTGGGGGC TATCGAGAGG CTGGAGGTCA   541 GGGCGGAGGC GCTTTCTTCA GCCCGAGCCC TGGCAGCAGC AGCCTGTCTT CGTGGAGCTT   601 CTTCTCGGAC GCCTCCGACG AGGCAGCCCT GTATGCAGCG TGCGACGAGG TGGAGTCTGA   661 ACTTAATGAA GCAGCCTCCC GTTTTGGCTT GAGCTCTCCG CTGCCCTCGC CCCGGGCTTC   721 TCCTAGGCCA TGGACCCCGG AGGATCCCTG GAGCCTATAC GGTCCAAGCT CGGGAGGCCG   781 AGCGCCAGAG GATAGCTGGC TACTCCTCAG CGCTCCTGGG CCCGTCCCAG CCTCCCCTCG   841 GCCTGCTTCT CCCTGCGGCA AGCGGCGCTA TTCCAGTTCC GGAACCCCAT CTTCGGCCTC   901 CCCAGCTTTG TCCCGCCGAG GCAGCCTTGG GGAAGAAGGT CCAGAGCCAC CTCCACCACC   961 CCCGTTGCCT CTGGTCCCGG ACCTAGTTCC CCTGGCCCCT TTTGACTATG TGGTGCTCAC  1021 ACGAGAGCAT CCCTCAGAAA CTCGGAAGAC TCTAGCGAGC AGGCAGTGTC CTGCTTCGGT 1081 CTGGAGAGCT CTCGTGCATG AAGCCTACCC TTCGTTACGA GCACTCTGGT GGGCATGC //
Primer: pCA-bgter Sequence file: RDB12860_A4Iob.seq
>RDB12860_1_pCA_bgter_A11_02.ab1     1 AGCCAAGAAG TCCAATAAAT TTTTGGCAGA GGGAAAAAGA TCTCAGTGGT ATTTGTGAGC    61 CAGGGCATTG GCCACACCAG CCACCACCTT CTGATAGGCA GCCTGCACCT GAGGAGTGCG   121 GCCGCGATGG TACCGTCGAC TAAGCTTGCG GCCGCTCAGG CAGGAGGCTC TTCTCCAGGA   181 CGTGCAGGGA AGCCACTCAG GTCTCGGCCA ATGATCTCAC TCACTTCCTC CAGGGTGATA   241 CCCTGGATAG GTACACTGTC TCTGAAGCCG CTGCTGTAGC CACCCCTGGC TTTCTCCTGC   301 TCCGAAGCCC CCTCCCCAGG GGTATAGCCT GGCCCCACCT CATTGTAGCC CTCAGGAGGT   361 AAGGGGTGGA TGGCACTCTG GGGATGGAAG GGGTCTTCGA GTGGTGGGGA GGAAGGCAGT   421 GGCGGCCTAA AGGGGGCTGG AGGGGAGAAT GGAAGCCCCA GGGCAAAAGA GGAGCCCTGT   481 CCTCCATATT GATCACCAGG AAAGGGGCGC GGAAGGAAAG AGACTGAAGG TAGGCGGGCA   541 CAACCTGTGG TACCCCCAGT CTCAGGGAAC ATCCGCAGGC CAGATCTATA GGACGGTGGG   601 GGCCCCGTCT GGGGGTACAG AGCGGGTGTG CTATAACCAA AGCCTTCTGA CAGATAAGGA   661 GTCTCATAAG CAGGGTCTTC ATGGGGATAG GAGGGGTAAG GGGGCCTGGG CGGTGAGAAG   721 TCCACGTCAG GGCCAAAGGG GGGACCCGAT GTGGAAGGGA AGCCCCGGAG AGATGAGTCT   781 GGTAGGGGCT CCTCCTTGAA GACCACAGGT AGGAACTTGA AACTTTGGGT AGGACTGCGC   841 TTCCTCCGTC CATTGGAGAC GTAAAAGTAG ACCTGGACTG GTCGGGACAC CCGCTTGTTG   901 CTGTACTCGG GGATGGTGAG AGTCAATGTC ACCTCACTGC TCTGCAGTCG GGTTCACCGC   961 TGCCTCTTCC TCCCACTGCA GTTTTCCATC AGACCCCTCT CAATGAACAC CACTTTAGAG  1021 TCTGGCAGGA AGTGGACCAG TCAACACTAG CTCTCTCTCT CTCACAGAGC AGCACTGGCT  1081 GTAGTCTCAC TGGGGCAGCT CTGGGCGACG CTGGAGCACT CGAATGGACA CTGATGCCTG 1141 GCTGCACGAA CACGAAACCA TTTCCG //
Primer: pAxCA_F1 Sequence file: RDB12860_A4Ioc.seq
>RDB12860_1_pAxCA_F1_H10_22.ab1     1 CGGTCCTTGT CTACATGTTC ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT    61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGGATCCGG TCGCCACCAT   121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG   181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG   241 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT   301 CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA   361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT   421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT   481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA   541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG   601 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   661 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   721 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT   781 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG   841 ACTCAGATCT CGAGCTATGG GGGCCGCAAG CTGCGAGGAT GAGGAGCTGG AATTTAAGCT   901 GGTGTTCGGA GAGGAAAAGG AGCCCCCCCC CGCTGGGCCC TGGGGGTCCT GGGGAAAGAG   961 CTGGACTCAG AGGATACCCC TCCATGCTGT CGTTTGGCCC TGGGGGGAAC CCCTTTCCTA  1021 TGGTGCTGCC CCTATTGGGA TTCCCCGACC CCACCCCTCG TCAGGAATGC ACTCACACGG  1081 CCCCGGTTCC CGCCCCATCA CTGCACCTGG GAGAGTCAGG CCAGCTTAGG TTCGTGAGTC 1141 CTGAGTCAGA GTTAATTGCC AATGGAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl