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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Smpd3

Expression clone of mouse Smpd3 cDNA

Catalog number RDB12795
Resource name pEF-Smpd3
Clone info. Expression clone of mouse Smpd3 cDNA.
Comment Expression was confirmed by the depositor with RT-PCR and immunoblotting with anti-Smpd3 antibody.
Vector backbone pEF-DEST51 (Plasmid)
Size of vector backbone 7.46 kb
Selectable markers Amp^r (E. coli) Bsd^r (mammalian cells).
Gene/insert name Mouse Smpd3 cDNA
Depositor|Developer Maeda, Shingo |
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External Database
Mouse Smpd3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (J Biol Chem. 289(12), 8135-8150(2014)).
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J Biol Chem. 289(12), 8135-8150(2014))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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          RDB12795 pEF-Smpd3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kakoi, H., Bone morphogenic protein (BMP) signaling up-regulates neutral sphingomyelinase 2 to suppress chondrocyte maturation via the Akt protein signaling pathway as a negative feedback mechanism. J. Biol. Chem. 289 (12): 8135-8150 (2014). PMID 24505141.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12795_A4F5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxEF_F2
          Sequence file: RDB12795_A4F5a.seq check
          >12795_1_pAXEF_F2_A01_01.ab1
              1 AATCATCATT CAGTGTCGTG AGGATTAGCT TGGTACTAAT ACGACTCACT ATAGGGAGAC
             61 CCAAGCTGGC TAGGTAAGCT TGATCAACAA GTTTGTACAA AAAAGCAGGC TCCGCGGCCG
            121 CCCCCTTCAC CATGGTTTTG TACACGACCC CCTTTCCTAA TAGCTGTCTG TCTGCCCTCC
            181 ACGCCGTGTC CTGGGCCCTC ATCTTCCCAT GTTACTGGCT GGTGGACCGG CTGTTGGCCT
            241 CTTTCATACC CACCACCTAT GAGAAGCGCC AGAGGGCAGA TGACCCCTGC TGCCTGCAGC
            301 TGTTCTGCAC GGTCCTCTTC ACGCCAGTCT ACCTGGCTCT GCTTGTGGCT GCACTGCCCT
            361 TTGCCTTTCT CGGGTTCATC TTCTGGTCTC CACTGCAGTC TGCCCGCCGG CCCTACTCCT
            421 ACTCCCGGCT AGAAGACAAG AACCCAGCTG GTGGGGCAGC CCTGCTTAGT GAATGGAAGG
            481 GCACGGGGGC AGGCAAAAGC TTTTGCTTCG CCACGGCCAA TGTCTGCCTC CTCCCCGACT
            541 CACTCGCGAG GCTCAATAAT GTGTTCAACA CTCAAGCTCG GGCCAAAGAG ATTGGGCAGA
            601 GAATCCGCAA TGGGGCCGCC AGGCCTCAGA TCAAGATCTA CATCGATTCT CCCACCAACA
            661 CCTCCATCAG TGCAGCCAGC TTCAGCAGCC TGGTGTCGCC ACAGGGTGGT GATGGATCCA
            721 GAGCTGTTCC TGGGAGCATT AAGAGGACAG CCTCTGTGGA ATACAAGGGG GATGGTGGGC
            781 GTCACCCCAG CGACGAGGCT GCCAACGGCC CAGCCTCTGG AGAACAGGCT GACGGAAGCC
            841 TTGAGGACAG CTGCATTGTG CGAATTGGTG GCGAGGAAGG AGGTCGGCCA CAAGAAGCTG
            901 ATGACCCTGC TGCCGGGAGC CAAGCCAGGA ATGGGGCTGG TGGGACCCCA AAGGGCCCAG
            961 ACACCCAACC ACAATCAGCG AGATGGGGAT TCTGGGGAGC CCTGGGCAGC CCCTCAGTCT
           1021 CAGGAGTCCT TTGGTGAGCA CGGCTGGGCA GACAGTGGTG CAGCCGGAAG CAAGGGGCTA
          1081 CAGCAAGCTG CTGTATAAGA CTTCTGTGGT GAAGAAAGGC AGCAAGCCCG CA
          //
          Primer: BGH_rev
          Sequence file: RDB12795_A4F5b.seq check
          >12795_1_BGHrev_B01_04.ab1
              1 CTTTCTCATG GTGATGGTGA TGATGACCGG TACGCGTAGA ATCGAGACCG AGGAGAGGGT
             61 TAGGGATAGG CTTACCTTCG AACCGCGGGC CCTCTAGATC AACCACTTTG TACAAGAAAG
            121 CTGGGTCGGC GCGCCCACCC TTTGCCTCCT CTTCCCCTGC AGACACCATC AGCCGCATGG
            181 CCACGGGGAG GTGGTCGGTC AGGCCGGACA GCTGGGTGAT AAAACTGAAT TCTTCTACCT
            241 CAGCCTTCCA GTCAGGGCAC AGCCCCTCCT CGGCGTGCAG CATGTAGTCG ATGCGGCGGC
            301 CATTGCCCTT CAGCAGATCC TTCCGGCCCT TCTGCCCAGC ACCTGGGCTC TTGCTGGTGG
            361 GGAAGGCCAG ATACTCCCTG CGGCCTTCCT CGCTCTCCAA CACCTTTTGA AGGTTGTCCG
            421 GGGTACACAC ATCCTCATCA TAGAGACCGT TTGTGTCCAG CAGGGTGCCA ATGGCCCAAG
            481 GCTTCTCCTC CCCGGGCCCA AGGCGGCAGG GGTCTTTGTA GCGAGTAAAG AGCGAGTGCT
            541 GCTGCTCCAG TTTGTCATCA GAAGAGCAGT TATCAAAGTT CAAATCTCCA CAGATGACGT
            601 CAAACACCAC CAGCTCCTCG GGGTTGGCTG TGCTGGTCGA GGAGGTAGAT TTTCGGAAAT
            661 CAGCCAGCCA GTCCTGAAGC AGGTCCAGCT GCTCACACCG GACGGCACTG TCCTCTGGTG
            721 GGGCATGCAG GTGTGTGCAG GCGATGTACC CAACAATTCT TTGGTCCTGA GGTGTGCTTC
            781 CCACCTGCAC CTTGAGAAAC AGAGCTCCCT TAGAGGCCAG GGCATCGAAG CTGCACCCAT
            841 TGGGGTAACA GTGATAGGCC ACGTCCATGA CAGGGTAGCG GCTGGCGAAG AAGAGACCGC
            901 TGTTGAGACA TTTGAAATTG CAGCAACCAT GACAACCGTA GACCCCAACA TCATACAGGA
            961 TGTACTCGAA GTAGCCGTGT AGCTGCTCTT TCAACTTAGC AGCCGCACGC TTTGTCAAAA
           1021 CACCTCCCTG CAGGCACAGG AAAGTCCAGA TTGGCCCGGG GAGAAGCTGA GACTCGTGAT
           1081 CAAGGCTTCA TCAAGTGCGC TTCTTGCGGG CCTGCCTGCC CTTCTTTCTC ACCCCACCAG
          1141 AAGAGGGTCT
          //
          Primer: SV40pro_F
          Sequence file: RDB12795_A4F5c.seq check
          >12795_1_SV40pro_F_A04_01.ab1
              1 GCCCCCTTCA CCGTAGCATT TCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
             61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
            121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
            181 TGGCCAAGCC TTTGTCTCAA GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA
            241 ACAGCATCCC CATCTCTGAA GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC
            301 GCATCTTCAC TGGTGTCAAT GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG
            361 TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA
            421 ATGAGAACAG GGGCATCTTG AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC
            481 ATCCTGGGAT CAAAGCCATA GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC
            541 GTGAATTGCT GCCCTCTGGT TATGTGTGGG AGGGCTAAGC ACTTCGTGGC CGAGGAGCAG
            601 GACTGACACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC
            661 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG
            721 TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
            781 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
            841 CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA
            901 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
            961 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
           1021 ATTTGCGTTG CGCTCACTGC CCCGCTTTCC AGTCGGGAAA CCCTGTCGTG CCAGCTGCAT
           1081 TATTGAATCG GCCCAACGCG CCGGAGAGCG TTTTGCGTAT TGGGCGGCCT CTCGGCTCAT
           1141 CGCCTCACTG ACTCGCCTGC GCCTCGGTCG GATCGACTTG CCGGCCGAGC CGGGATAATT
          1201 CCAGGCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakoi, H., Bone morphogenic protein (BMP) signaling up-regulates neutral sphingomyelinase 2 to suppress chondrocyte maturation via the Akt protein signaling pathway as a negative feedback mechanism. J. Biol. Chem. 289 (12): 8135-8150 (2014). PMID 24505141.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl