Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBR-lacZ alpha

Plasmid clone for quantitative assay for genome editing

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Biology Open, 2, 363-367 (2013)).
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biology Open, 2, 363-367 (2013))。
提供依頼	提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12261_A6Hup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB12261_A6Hua.seq
>12261_12261_A6Hu_1_T7_20bp_D08_11_ABI24.ab1     1 CGTAACATGG GCGCGTCGAG TCGGCGGTAT CGATAAGCGT GTGATATCGA GATTCCTGCA    61 GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT   121 CCCTTTAGTG AGGGTTAATT TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT   181 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG   241 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT   301 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC TAGCAGCACG CCATAGTGAC TGGCGATGCT   361 GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC   421 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA   481 CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT   541 GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT   601 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA   661 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA   721 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC   781 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC   841 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT   901 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC CGAAAGAACG   961 TTTTTCCCAA TGATGAGCAC TTTTTAAAGT TTCTGCTATG TGGGCGCGGG TATTTATCCC  1021 GTGTTTGACG CCGGGCAAGA GCAACTCGGT TCGGCGCATA CACTACCTCA GATTGACCTT 1081 GGTGAAGTAC CTCACCCAGG TTCCCACAGG A //
Primer: T3 Sequence file: RDB12261_A6Hub.seq
>12261_12261_A6Hu_1_T3_G08_20_ABI24.ab1     1 CAGGCTCACG CGGTGGCGGC CGCTCTAGAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA    61 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA   121 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG   181 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG   241 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG   301 CGCCCTGTAG CGAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG   361 GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT   421 TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC   481 TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA   541 ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC   601 CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG   661 CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT   721 GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG   781 CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC   841 GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT   901 TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCCA TTATGATTCT TCTCGCTTTC   961 CCGGCGGCAT CGGGATGCCC GCGTTTGCAG CCCATGCTGT CCAGGGCAGT AGATGACGAA  1021 CCAATCAGGA CAGGCCTCAA TGATCGCCTC CGCGCCTCTA CAGTCTACCT TTCGAATCAT 1081 TGGCAAACCG CCTGG //
Primer: ColE1ori_F Sequence file: RDB12261_A6Huc.seq
>12261_12261_A6Hu_1_ColE1ori_F_B09_06_ABI24.ab1     1 TTCTTTTATC TGATCGTCGC ACCGTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG    61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT   121 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA   181 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT   241 CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG   301 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA   361 GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC   421 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC   481 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC   541 GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG   601 TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA   661 GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG   721 ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGG   781 TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT   841 GGATGCGGCG GGACCAGAGA AAAATCACTC AGGTCAATGC CAGCGCTTCG TTTAATACAG   901 ATGTAAGTGT TCCACAGGGT AGCCCAGCAG CATCCTGCGA TGCAGATCCG GGAACATATG   961 GGTGCAGGCG CTGAACTTTC GGCGTTTCAG ACTTTACGGA AACACGGAAC GAGGACATTC  1021 ATGTTGGTGC TCAGTTCGCA GACGTTTTGC AGCAGCAGCT CGCTCAACGG ATCGCTTCGG 1081 CA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl