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pcDNA3.1-pA83-Lats2KD

Expression clone of mouse Lats2 cDNA, kinase dead variant

Catalog number RDB12201
Resource name pcDNA3.1-pA83-Lats2KD
Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. N-terminal HA-tag.
Comment D767A mutation in kinase subdomain VII, corresponding human D809A mutation. The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Lats2 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Lats2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          RDB12201 pcDNA3.1-pA83-Lats2KD DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12201_A4Fup1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: HA tag, insert 5'
          Sequence file: RDB12201_A4Fua.seq check
          >12201_12201_A4Fu_CMV-Foward_A09_03.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNANNTGGG NGGNANGCGT GTACGGTGGG
             61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
            121 AAATTAATAC GACTCACTAT AGGGAGACCC ANNNNNNNTA GTTAAGCTTG GTACCGAGCT
            181 CGGATCCACT AGTCCAGTGT GGTGGAATTG ATCCCAGTGT GGTGGTACGG GAATTCCTGC
            241 AGGATGTACC CATACGACGT TCCAGATTAC GCTGGTACCA TGAGGCCAAA GACTTTTCCT
            301 GCCACAACTT ACTCTGGAAA TAGCCGGCAG CGATTGCAAG AGATTCGAGA GGGGCTGAAG
            361 CAGCCATCCA AGGCTTCCAC CCAGGGGCTG CTGGTGGGAC CAAACAGTGA CACTTCCCTG
            421 GATGCCAAAG TCCTGGGGAG CAAAGATGCC TCCAGGCAGC AGCAAATGAG AGCCACCCCG
            481 AAGTTTGGAC CTTATCAAAA AGCTCTCAGG GAAATCCGAT ATTCCCTCCT GCCTTTTGCC
            541 AACGAGTCAG GCACTTCGGC AGCTGCAGAG GTGAACCGGC AGATGCTTCA GGAGTTGGTG
            601 AATGCGGGAT GTGACCAGGA GATGGCTGGC AGAGCGNNNN NNCAGACGGG CAGTAGGAGT
            661 ATCGAAGCTG CCTTGGAGTA CATCAGTAAG ATGGGCTACC TGGACCCCAG GAATGAGCAG
            721 ATTGTGCGAG TCATCAAGCA GANCTCCCCA GGAAAAGGGN CTGGCGCCCA CCCCGGTGAC
            781 TCNGCGGCCC ANTTTCGAGG GCACAGGGGA AGCACTCCCA TCCTACCNNN AGCTGGGTGG
            841 TGCAAACTAC NANGGCCCCG CCGCANTGGA NGANATGCCG CGGCANTATT TNAGACTTTN
            901 NCNTCCCTGN NNCCGGAGCC NGGCANCNNN NGNNGCCCAG NCTCNNNAGC ANNNNNCNNA
            961 ANNGGNNNNA GCACANNANN AGANNCNANN GCGNNANTTT NNNNGGNCNN NNCNCNNNNN
          1021 GNTNNNNNNN NNN
          //
          Primer: GNP Primer #10
          Region: TRF2-3'UTR+polyA83, insert3'
          Sequence file: RDB12201_A4Fub.seq check
          >12201_12201_A4Fu_GNP_primer#10_B09_06.ab1
              1 NNNNNNNNNN NNNNATTTNN ANNNNAACGT TTACNGGTTC TTTGGTGCCC ATAGTCGAGC
             61 GGCCGCCACT GTGCTGGATA TCTGCAGAAT TTCGAGGTTA CACGTACACC GGCTGGCAGC
            121 CCTCGGCCGC ACCTTCCAAG TCCGCATCCC CTGGGTCTGC ACTCTCTGCG GGCTCTGAGG
            181 GCTTCGGGCA CCGGAAGGGA TAGCCATTGT CATCGAAGAA CCTGCGGAAG GTGAACTCAT
            241 AGAAGGCGTG CTCTGGATGC TTGCTGCTGG GGGAGGCCAG CGTGTCCCAG GCCTTGGCGC
            301 TCTCTCCGCT GGCCTCGTGC CAGGGGCTTT CTTCATCCAC CGGGTCAAAA TTGGAGGTGT
            361 CCATGGGGTG GCTGATGGTG GGGACGTAGG GTGCAGGCTG CTTTCGGATG TCACGGGAAA
            421 AGTCGATGGT GTTGAAGAAC GGGTGTGCCT TGAGGTCATC TGCCCCATCC CTGCCCAGGC
            481 GGCAGTCAGC CGCGCAGCAC AGCTTCGTGA TGAGGTCTCG GGCCTCAGCG CTGANCCTCA
            541 CCTGCGTANG GATATGCAGC GTGCTCTCCC AGTTGATCAC CTTCAGCTGC GTCTCTGTGG
            601 GGGTGGGGGN CAAGAAAGGN GGNTGNCNNA CCNGCATCTC NAAGAGAATC ACACCGACNN
            661 TNCNCCNNTC NNANANNTGC NNNNANCNTT NGNNNANAAN CANNNNCNNA NCGANGTNNT
          721 TNNNNNNNCC NANNNNANAN TGNNNNNNNN NN
          //
          Primer: Lats2#11
          Region: insert mid
          Sequence file: RDB12201_A4Fuc.seq check
          >12201_12201_A4Fu_Lats2#11_C02_08.ab1
              1 NNNNNNNNNN CNNNNNGCNN NNGCTGGNNC AGGNANNGGC CNAAGCTGGG CTCTGTGAGG
             61 CCGAGCAGGA GCAGATGAGG AAGATCCTCT ACCAGAAGGA GTCTAACTAC AACCGGCTGA
            121 AGAGGGCCAA GATGGACAAG TCCATGTTTG TGAAAATCAA GACTCTAGGC ATCGGTGCCT
            181 TTGGGGAAGT GTGCCTCGCT TGTAAGCTGG ACACTCACGC TCTGTACGCC ATGAAGACTC
            241 TCAGGAAGAA GGATGTCCTG AACCGGAATC AAGTGGCCCA TGTCAAGGCT GAGAGGGACA
            301 TCCTGGCTGA AGCAGACAAT GAGTGGGTGG TCAAACTCTA CTACTCCTTC CAGGACAAGG
            361 ACAGCCTGTA CTTTGTGATG GACTACATAC CAGGCGGGGA TATGATGAGC CTGCTGATCA
            421 GGATGGAGGT CTTCCCTGAG CACCTGGCCC GCTTCTACAT TGCAGAGTTG ACCCTGGCCA
            481 TTGAAAGTGT CCACAAGATG GGCTTTATCC ACCGGGACAT CAAGCCTGAC AACATACTCA
            541 TCGACCTGGA TGGTCATATT AAGCTGACAG CTTTTGGCCT CTGCACTGGA TTCAGGTGGA
            601 CTCACAATTC CAAGTACTAC CAGAAAGGGA ACCACATGAG ACAGGACAGC ATGGAGCCCG
            661 GTGACCTCTG GGACGATGTT TCCAACTGTC GCTGTGGAGA CAGGTTAAAG ACCCTGGAGC
            721 AGANGGCGCA GAAGCAGCAC CAGANGTGCC TGGCACATTC TCTTGTCGGG ACACCAAATT
            781 ACATCGCTCC NGANGTGCTT CTCCGCNAAG GGTACACGCA GCTCTGTGAC TGGTGGANCG
            841 TCGGTGTGAN TCTCTTTGAN ATGCTGGTTG GGCAGCCGCC TTTCNNGGCC NNCACCCCCN
            901 NCAGANNNNC ANCTGNNNNN TGATCAACTG GNNNANCNNN CNNNNNNTCC NNACNNNNGN
          961 NANGNNCANC GNNNNNNNCN NNNNNNNCAT CNCNAANNNN NNNNNN
          //
          Primer: BGH_rev2
          Region: TRF2-3'UTR_polyA83
          Sequence file: RDB12201_A4Fud.seq check
          >12201_12201_B0CpF_BGH_rev2_C04_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGNGGNNG GGCNAACNAC AGATGGCTGG CAACTAGAAG
             61 GCACAGTCGA GGCTGATCNN CGGGTTTAAA CTCAATGGTG ATGGTGATGA TGACCGGTAT
            121 GCATATTCAG ATCCTCTTCT GAGATGAGTT TTTGTTCGAA GGGCCCTCTA GACTCGAGGT
            181 ACGCCACAGC GTATGATGCG TATTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
          241 TTTTTTTTTT TTTTTTTTTT TTTTNNNNNN NNNN
          //
          Primer: SV40pro_F
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB12201_A4Fue.seq check
          >12201_12201_A4Fu_SV40pro_F_G10_19.ab1
              1 CCCTGTCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            841 CTTCCTCGTG CTTTACGGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTTCTG AGCGGGACTC TGGGGTTTCG CGAAATGACC CGAACCCAAG
            961 CGAACGCCCA TCCTGCCCAT CACGAGATTT CCGATTCGAT CGGCCGCCGT TCTTATGAAA
          1021 TGTTCGGCTT CGTATTCGAT TTTCCAGGAA CGATC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl