Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1-pA83-Tead4VP16

Expression clone of mouse Tead4, constitutive active

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12186_A4Fup1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,T7 pro,6XHis,T7 tag,Xpress tag,insert 5'(Tead4_VP16) Sequence file: RDB12186_A4Fua.seq
>12186_12186_A4Fu_CMV-Foward_A08_02.ab1     1 AGTTCACTCC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG   121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCACTA   181 GTCCAGTGTG GTGGAATTGC CACCATGGGG GGTTCTCATC ATCATCATCA TCATGGTATG   241 GCTAGCATGA CTGGTGGACA GCAAATGGGT CGGGATCTGT ACGACGATGA CGATAAGGTA   301 CCAGGATCCA GTGTGGTGGA ATTCATTACC TCCAACGAGT GGAGCTCTCC CGACTCCCCC   361 GAGGGGAGCA GCATCTCTGG GGGCAGCCAG GCACTGGACA AGCCCATCGA CAATGATGCA   421 GAGGGTGTAT GGAGCCCCGA AATTGAGCGA AGCTTCCAGG AGGCCCTGGC CATCTACCCA   481 CCCTGCGGCC GCCGCAAAAT TATCCTGACG GAGGAAGGCA AGATGTATGG TCGGAATGAG   541 CTGATCGCAC GCCATATCAA GCTCAGGACA GGGAAGACGC GCACAAGGAA GCAGGTCTCC   601 AGCCACATCC AGGTGCTTGC CCGTCGAAAA GCCCGGGAGA TCCAGGCCAA ACTCAAGGAC   661 CAGGCAGCTA AGAACAAGGC CCTGCAGAGC ATGGCTGCCA TGTCGTCCGC ACAGATCGTC   721 TCGGCCACAG CCTTCCACAG TAAAATGGCT CTTGCCCGGG GCCCTGGCTA CCCAATCGAT   781 GCCCCCCCGA CCGATGTCAG CCTGGGGGAC GAGCTCCACT TAGACGGCGA GGACGTGGCG   841 ATGGCGCATG CCGACGCGCT AGACGATTTC GATCTGGACA TGTTGGGGGG ACGGGGGATT   901 CCCCGGGTCC GGGATTTACC CCCCCACGAC TCCGCCCCCT ACGGCGCTCT GGATATGGCC   961 GACTTCGAGT TTGAGCAGAT GTTTACCGAT GCCCTTGGGA AATTGACGAG TACGGTGGGT  1021 AGGGATATCC AGCACAGTGG CGGGCCCGCT CGACTATGGG CAACCAAAGA CCCTGTAACG 1081 TTTATCCTTT TTAATGGAAT GTGCACGAAT AAAGTTTGGC AAAGGCA //
Primer: GNP Primer #10 Region: TRF2 3'-UTR and pA.insert (Tead4_VP16),Xpress tag,T7 tag,6XHis Sequence file: RDB12186_A4Fub.seq
>12186_12186_A4Fu_GNPprimer#10_E02_14.ab1     1 TTAATTTGTG CAATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA    61 GCGGCCGCCA CTGTGCTGGA TATCCTACCC ACCGTACTCG TCAATTCCAA GGGCATCGGT   121 AAACATCTGC TCAAACTCGA AGTCGGCCAT ATCCAGAGCG CCGTAGGGGG CGGAGTCGTG   181 GGGGGTAAAT CCCGGACCCG GGGAATCCCC GTCCCCCAAC ATGTCCAGAT CGAAATCGTC   241 TAGCGCGTCG GCATGCGCCA TCGCCACGTC CTCGCCGTCT AAGTGGAGCT CGTCCCCCAG   301 GCTGACATCG GTCGGGGGGG CATCGATTGG GTAGCCAGGG CCCCGGGCAA GAGCCATTTT   361 ACTGTGGAAG GCTGTGGCCG AGACGATCTG TGCGGACGAC ATGGCAGCCA TGCTCTGCAG   421 GGCCTTGTTC TTAGCTGCCT GGTCCTTGAG TTTGGCCTGG ATCTCCCGGG CTTTTCGACG   481 GGCAAGCACC TGGATGTGGC TGGAGACCTG CTTCCTTGTG CGCGTCTTCC CTGTCCTGAG   541 CTTGATATGG CGTGCGATCA GCTCATTCCG ACCATACATC TTGCCTTCCT CCGTCAGGAT   601 AATTTTGCGG CGGCCGCAGG GTGGGTAGAT GGCCAGGGCC TCCTGGAAGC TTCGCTCAAT   661 TTCGGGGCTC CATACACCCT CTGCATCATT GTCGATGGGC TTGTCCAGTG CCTGGCTGCC   721 CCCAGAGATG CTGCTCCCCT CGGGGGAGTC GGGAGAGCTC CACTCGTTGG AGGTAATGAA   781 TTCCACCACA CTGGATCCTG GTACCTTATC GTCATCGTCG TACAGATCCC GACCCATTTG   841 CTGTCCACCA GTCATGCTAG CCATACCATG ATGATGATGA TGATGAGAAC CCCCCATGGT   901 GGCAATTCCA CCACACTGGA CTAGTGGATC CGAGCTCGGT ACCAAGCTTT AACTAGCCAG   961 CTTGGGTCTC CCTATAGTGA GTCGTATTAA TTTCGATAAG CCAGTAAGCA GTGGTCTCTA  1021 GTTAGCCCAG AGAGCTCTGC TTATATAGAC CTCCCCACCG TACACCGCCT ACGCCATTGC  1081 GTCAATGGGG GCGGAGTGTA CGACATTTGA AGGTCGATTG ATTGGTTCAA CATTCCATGA 1141 CGCTTCCAAT G //
Primer: BGH_rev2 Region: bGHpA,TRF2 3'-UTR and pA Sequence file: RDB12186_A4Fuc.seq
>12186_12186_A4Fu_BGH_rev2_F02_17.ab1     1 GTAGACTGAG GGGTTAAGGG GGGGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT    61 AGAAGGCACA GTCGAGGCTG ATCAGCGGGT TTAAACTCAA TGGTGATGGT GATGATGACC   121 GGTATGCATA TTCAGATCCT CTTCTGAGAT GAGTTTTTGT TCGAAGGGCC CTCTAGACTC   181 GAGGTACGCC ACAGCGTATG ATGCGTATTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT   241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TCTTTCCCCA AATTTTTTTT   301 TGGCCCCTTC AATTTAAAAA AAAAAATTTT TCCGGTTTTT TTGGGCCCCA ATACCAACCC   361 CCCCCCCGGG GGGGGAATTT CCCCCCCCCG GACCCCCTAT TTTCAGGGGG GCGGGGAAAA   421 CTTTCCCCAC AACCCAAAAT GCGCCAATTC CAAAACCCCC TTGGGGGGGG GAACCCGGGG   481 GGGGAAAATC CCGGGACCGG GGGAAAACCC CCCCCCCCCA ATTTCAAAAA AAAAAATTTT   541 AAGGGGGGGG GGGGCCCCCC CCCCCCCCCC CCCCCTAAAA GGAGACCCCC CCCCCCCATT   601 GAAAACGGGG GGGGGGCCCA ATTGGTAACC CGGGCCCCCC GGCAAAAAAC CTTTTTTTTG   661 GAAAGGGGGG GGGAAAAAAA AATTTTGGGA AAAAATGAAC CCTTCCTTTG GGGGGCTTTT   721 TTTATCCCCC GGCCTTGATT GGGGGATATC CCGGGTTTTT TAGGGGAAAC CCTGAGGTTG   781 GGTGTGAGAG CCTTTTTTTG GGCGCCTCTC CTCTGCCGCG AGAGTATGGG GGGGGAGACT   841 CTCTTCCACA CAACTTTTCT CTTCTCCCCG AGGATATATT TTGCCCGCCG CGCGGGGGAA   901 TAGACGCGCC TCGGAAACAT CTCTTTGTGG GGGCTCCCAC CCCTACTTTA TGAGAGAGTT   961 CACGACGGCG CCACACAATA CTCTCTCCTC TGCGGGAGCG AGAACCACCC TCGACGGGTA 1021 GTGATACTAC ACGCGAGATG ATGGTGCTC //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB12186_A4Fud.seq
>12186_12186_A4Fu_SV40pro_F_H02_23.ab1     1 CCCGGGTCCC TTTAACATTC AGGAATAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG    61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG   121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA   181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT   241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT   301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG   361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT   421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC   481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC   541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA   601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG   661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT   721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG   781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGGC GGCGAATGGG   841 CTGACCGCTT CCTCGTGCTT TACGGGTATC GCCGCTTCCG ATTCGCAGCG CATCGCCCTT   901 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGGTTCGCGA AATGACCGAA   961 CCAAGCGACG CCCCAACCTG CCATCACGAG GATTTCCGAA TTCCACCGGC CGCCCTTCTT  1021 ATGAAACGGT TGGGGCTTCG GAATCGGTTT CAGAACGTCC GGGCTGGGAA TGAATCTTCA  1081 AGCGCGGGGA TCTCAATGGC TGGAGTCCTT CGCCAACCCC AACCTTGGTT TATCTGGCAG 1141 ACCCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl