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pPyCAG-BstXI-HygP2AEGFP

Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.

Catalog number RDB12108
Resource name pPyCAG-BstXI-HygP2AEGFP
Clone info. Expression vector with CAG promoter, HA-tag, Hygromycin selection. GFP marker.
Vector backbone Plasmid
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Niwa, Hitoshi |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence RDB12108z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12108 pPyCAG-BstXI-HygP2AEGFP DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pPyCAG-BstXI-HygP2AEGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12108).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12108_A3E7p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: IRES-F (Pr0036)
            Region: IRES,HygR 5'
            Sequence file: RDB12108_A3E7a.seq check
            >12108_12108_A3E7_1_IRES-F_A01_01_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNGTCGANGT TNNAAAAACG TCTAGGCCCC CCGAACCACG
               61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GAAAAAGCCT
              121 GAACTCACCG CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC
              181 CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT
              241 GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTCAT
              301 CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC
              361 GAGAGCCTGA CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT
              421 GAAACCGAAC TGCCCGCTGT TCTGCAGCCG GTCGCGGAGG CCATGGATGC GATCGCTGCG
              481 GCCGATCTTA GCCAGACGAG CGGGTTCGGC CCATTCGGAC CGCAAGGAAT CGGTCAATAC
              541 ACTACGTGGC GTGATTTCAT ATGCGCGATT GCTGATCCCC ATGTGTATCA CTGGCAAACT
              601 GTGATGGACG ACACCGTCAG TGCGTCCGTC GCGCAGGCTC TCGATGAGCT GATGCTTTGG
              661 GCCGAGGACT GCCCCGAAGT CCGGCACCTC GTGCACGCGG ATTTCGGCTC CAACAATGTC
              721 CTGACGGACA ATGGCCGCAT AACAGCGGTC ATTGACTGGA GCGAGGCGAT GTTCGGGGAT
              781 TCCCAATACG AGGTCGCCAA CATCTTCTTC TGGANGCCGT GGTTGGCTTG TATGGAGCAG
              841 CAGANGCGCT ACTTCGAGCG GANGCATCCG GANCTTGCAN GNNCGCCGCG GCTCCNGGCG
              901 TATATGCTCC GCATTGGNCT NGANCNACTC TATCANANCT TGGNTGANGG CNNNTTCNAT
              961 GANGCANCTT GGGNGCNNGN NCNNTGCGAC GCNNNNGNCC NNTNCNNNNN NCNNGNNNNN
            1021 NNNGNNNNNN NNNN
            //
            Primer: HygR-C_F (Pr0501)
            Region: HygR 3',P2A,EGFP 5'
            Sequence file: RDB12108_A3E7b.seq check
            >12108_12108_A3E7_1_HygR-C_F_B01_04_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGNNGTCGC CNACATCTTC TTCTGGAGGC CGTGGTTGGC
               61 TTGTATGGAG CNNCAGACGC GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC
              121 GCGGCTCCGG GCGTATATGC TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA
              181 CGGCAATTTC GATGATGCAG CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG
              241 AGCCGGGACT GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG
              301 CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA
              361 GGAAATGCAT GCCACCAACT TCTCCCTGCT GAAGCAGGCC GGCGACGTGG AGGAGAACCC
              421 CGGCCCCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
              481 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
              541 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
              601 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
              661 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
              721 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
              781 CACCCTGGTG AACCGCATCG AGCTGANNGG CATCGACTTC AANGAGGANG GCAACATCCT
              841 GGGGCACAAG CTGGANTACA ACTACNACAG CCACAACGTC TATATCATGG CCGANAAGCA
              901 GAANNAACGG CATCNAGGNG AACNTCNAGN TNCGCNNCAA CATCNANGNA NGGNAGCNNG
            961 CANCNCGCNN ANCACTACCA NCAGANNNCC CCCNNTCGGN NNNNNNCCNN NNNNNNNN
            //
            Primer: pCAG-F_V3 (Pr0495)
            Region: BstXI stuffer,IRES
            Sequence file: RDB12108_A3E7c.seq check
            >12108_12108_A3E7_1_pCAG-F_V3_C01_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNANNNCG GGGTNCGGCT TCTGGCGTGT
               61 GANCGGNNNN NCNNNAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT
              121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCCTCGAGAT
              181 CCATTGTGCT GGCGGTAGGC GAGCAGCGCC TGCCTGAAGC TGCGGGCATT CCCGATCAGA
              241 AATGAGCGCC AGTCGTCGTC GGCTCTCGGC ACCGAATGCG TATGATTCTC CGCCAGCATG
              301 GCTTCGGCCA GTGCGTCGAG CAGCGCCCGC TTGTTCCTGA AGTGCCAGTA AAGCGCCGGC
              361 TGCTGAACCC CCAACCGTTC CGCCAGTTTG CGTGTCGTCA GACCGTCTAC GCCGACCTCG
              421 TTCAACAGGT CCAGGGCGGC ACGGATCACT GTATTCGGCT GCAACTTTGT CATGCTTGAC
              481 ACTTTATCAC TGATAAACAT AATATGTCCA CCAACTTATC AGTGATAAAG AATCCGCGCC
              541 AGCACAATGG ATCTCGAGGA ATTCACTCCT CAGGTGCAGG CTGCCTATCA GAAGGTGGTG
              601 GCTGGTGTGG CCAACTGGCT CACAAATACC ACTGAGATCG ACGGTATCGA GCGGCCGCGG
              661 TTATTTTCCA CCATATTGCC GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC
              721 TTCTTGANNA NNNNNNCTAG GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG
              781 AATGTCGTGA ANGAAGCAGT TCCTCTGGAA GCTTCTTGAN NNCAAACAAC GTCTGTNNCG
              841 ACCCTTNGCA NGCAGCGNAN NCCCCCCACC TGGNGACNGG TGCNTCTGCN NNCNAAANNC
            901 NNCGNGNATN AAGANACACN TGCANAGGCG GNACNACCCC NNGNGCNNCN NNNNNNNNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: EGFP 3'
            Sequence file: RDB12108_A3E7d.seq check
            >12108_12108_A3E7_1_bGH_rev3_D01_10_ABI24.ab1
                1 NNNNNNNNNN NNNGNNNGGG GGNNGGGCAN CAACNGANGG CTGGCAACTA GAAGGCACAG
               61 TCGAGGCTGN TCAGCGAGCT CTAGATCATC GATTACTTGT ACAGCTCGTC CATGCCGAGA
              121 GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG
              181 TCTTTGCTCA GGGCGGACTG GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG
              241 TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG
              301 TTGTGGCGGA TCTTGAAGTT CACCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG
              361 ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG
              421 AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG
              481 GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT
              541 TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG
              601 CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG
              661 GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTANGTGG CATCGCCCTC GCCCTCGCCG
              721 GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC
              781 CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGGGNNNG NGTTCTCCTC CACGTCNCCN
              841 GCCTGCTTCA NCNNGNANAA GTTGGTGGCA TGCATTTCCT TTGCCNNCNN ANGANTGCTG
              901 GGGGCGTCNN NTNNCACTAT CGGCNANTAC TTCTACACAG CCNTCNNNNC ANANNNCNNC
              961 GCTTCTGNNG GCGANTNGNN NACNNNNNNN NNCCNNNNTN CNNNANNNGN NNNANNNNNN
            1021 NNNN
            //
            Primer: IRES-M_R (Pr0406)
            Region: BstXI stuffer
            Sequence file: RDB12108_A3E7e.seq check
            >12108_12108_A3E7_1_IRES-M_R_E01_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNTGC TTCNTTNNCG ACNTTNNACN GACCTTGCAT TCCTTTGGCG
               61 AGAGGGGAAN NNNNCCCTAG GAATGCTCGT CAAGAAGACA GGGCCAGGTT TCCGGGCCCT
              121 CACATTGCCA AAAGACGGCA ATATGGTGGA AAATAACCGC GGCCGCTCGA TACCGTCGAT
              181 CTCANTGGTA TTTGTGAGCC AGTTGGCCAC ACCAGCCACC ACCTTCTGAT AGGCAGCCTG
              241 CACCTGAGGA GTGAATTCCT CGAGATCCAT TGTGCTGGCG CGGATTCTTT ATCACTGATA
              301 AGTTGGTGGA CATATTATGT TTATCAGTGA TAAAGTGTCA AGCATGACAA AGTTGCAGCC
              361 GAATACAGTG ATCCGTGCCG CCCTGGACCT GTTGAACGAG GTCGGCGTAG ACGGTCTGAC
              421 GACACGCAAA CTGGCGGAAC GGTTGGGGGT TCAGCAGCCG GCGCTTTACT GGCACTTCAG
              481 GAACAAGCGG GCGCTGCTCG ACGCACTGGC CGAAGCCATG CTGGCGGAGA ATCATACGCA
              541 TTCGGTGCCG AGAGCCGACG ACGACTGGCG CTCATTTCTG ATCGGGAATG CCCGCAGCTT
              601 CAGGCAGGCG CTGCTCGCCT ACCGCCAGCA CAATGGATCT CGAGGAATTC TTTGCNNNNN
            661 NNATGANNNN NNACNNNNNN NNNN
            //
            Primer: CAGen_R1 (Pr0616)
            Region: CAG pro,SV40 pA,polyoma ori
            Sequence file: RDB12108_A3E7f.seq check
            >12108_12108_A3E7_1_CAGen_R1_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNGGNCG GGGGTCGTTG GGCGGTCNGC CNGGCGGGCC ATTTACCGTA
               61 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC
              121 TATTAATAAC TAGTCAATAA TCAATGTCGA CGATGATATC AGATCCAGAC ATGATAAGAT
              181 ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG
              241 AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAATT
              301 CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG TAATTTTCCT CCCGACTCTT
              361 AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA ACTGACCGCA GCTGGCCGTG
              421 CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC AGAGGGCAGT GTGGTTTTGC
              481 AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT CAGTGTGGTT TTGCAAGAGG
              541 AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC CCAATCATTA CTATGACAAC
              601 AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGCCCCT GGCCTCCGCT TACTCTGGAG
              661 AAAAAGAAGA GAGGCTTCCA GAGGCAACTT GTCAAAACAG GACTGGCGCC TTGGAGGCGC
              721 TGTGGGGCCA CCCAAATTGA TATAATTAAG CCCCAACCGC CTCTTCCCGC CTCATTTCAG
              781 CCTCACCACT ATGTGAATAT AGCTGANTAC ACAGTTTATT GAATAAACAT TAATTTCNAG
              841 GAAATACAGT CTTTGTTTTT CCNAAGCGNN CATTGAAAAA GGAAGANTAT GANTATTCAA
              901 CATTTCCGNG TCGCCCTTNN NCCCNTTTTT TGNGGNATTT TGCNNTCCNG NTTTTGCNCN
            961 CCNNGAAANN CTNGNGAANN NNAANNNNGC TGNNNNNCAN TNGGNNNNNN NNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,SV40 pA,ori
            Sequence file: RDB12108_A3E7g.seq check
            >12108_12108_A3E7_1_SV40pro_ori_F_G01_19_ABI24.ab1
                1 NNNNNNNNNN GNNNNNGNNG CCTCGGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT
               61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT AACTTGTTTA TTGCAGCTTA TAATGGTTAC
              121 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT
              181 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGATCCGCTG CATTAATGAA
              241 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
              301 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
              361 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
              421 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
              481 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
              541 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
              601 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
              661 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
              721 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
              781 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAN
              841 CGAGGTATGT ANGCGGTGCT ACAGANTTCT TGAAGTGGTG GNCTAACTAC NGCTACNCTA
              901 NAAGAACAGT ANTTGGTATC TGCGCTCNNC TNNNNNCNGT TACCTTNNGA AAAAGANTNG
              961 GTANCNNNNG NTNCNNNNAA NNAANNNNCN NNNNNNNNCN GNNNGNNTTT TTNNNTNNNN
            1021 NNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.05.01

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