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Pgap-DSD-SY/L

Expression clone of DAP2 (cyt, lum)-SEC12 (tmd) chimeric protein under the control of TDH3 promoter in S. cerevisiae

Catalog number RDB08802
Resource name Pgap-DSD-SY/L
Alternative name 259
Clone info. Expression clone of DAP2 (cyt, lum)-SEC12 (tmd) chimeric protein under the control of TDH3 promoter in S. cerevisiae. S366L, Y367L mutations in TMD.
Comment DSD (Dap2 w/Sec12TMD-S366L, Y367L, TDH3 promoter) on pYO326 (2 micron, URA3)
Vector backbone pYO326 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae DAP2 S. cerevisiae SEC12
Depositor|Developer Nakano, Akihiko |
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External Database
S. cerevisiae DAP2 S. cerevisiae SEC12

        Reference sequence
          
         
        Sequence RDB08802z.seq
        Remarks, protocol and/or map (pdf) RDB08802.pdf

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Cell Biol. 152, 935-944, 2001).
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        提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Cell Biol. 152, 935-944, 2001)。
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        RDB08802 Pgap-DSD-SY/L Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The Pgap-DSD-SY/L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08802).

        Reference section:

        Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


        References and tips

        Featured content

        Featured content Akihiko Nakano's S. cerevisiae clones (English text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Sato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. [link to RRC of NBRP]

        2023.06.19

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