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p3xFLAG-CMV-14-hSVBP (CCDC23)

Expression construct of human SVBP (CCDC23) cDNA

Catalog number RDB08520
Resource name p3xFLAG-CMV-14-hSVBP (CCDC23)
Alternative name human small vasohibin binding protein
Clone info. Expression construct of human SVBP (CCDC23) cDNA. FLAG epitope tag sequence is translated with SVBP.
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r
Gene/insert name Human small vasohibin binding protein cDNA
Depositor|Developer SATO, Yasufumi |
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External Database
Human small vasohibin binding protein
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Suzuki, Y. et al., J. Cell Sci. 123, 3094-4101, 2010.).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki, Y. et al., J. Cell Sci. 123, 3094-4101, 2010.)。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08520 p3xFLAG-CMV-14-hSVBP (CCDC23) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The p3xFLAG-CMV-14-hSVBP (CCDC23) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08520).

          Reference section:

          Suzuki, Y., Kobayashi, M., Miyashita, H., Ohta, H., Sonoda, H., Sato, Y., Isolation of a small vasohibin-binding protein (SVBP) and its role in vasohibin secretion. J. Cell. Sci. 123, 3094-4101, (2010). PMID 20736312. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08520_A8Atp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB08520_A8Ata.seq check
          >08520_08520_A8At_2_CMV-Forward_B02_05_ABI24.ab1
              1 CTAAATTACC CGCCCGTTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CGTTTAGTGA ACCGTCAGAA TTAAGCTTGC GGCCGCCACC ATGGATCCAC
            121 CTGCACGTAA AGAAAAAACC AAAGTTAAAG AATCTGTCAG CAGAGTTGAG AAGGCCAAAC
            181 AGAAATCAGC CCAGCAGGAG CTGAAGCAGA GACAAAGAGC AGAGATCTAT GCCCTCAACA
            241 GAGTCATGAC AGAACTGGAG CAGCAGCAGT TTGATGAGTT CTGTAAACAG ATGCAGCCTC
            301 CTGGAGAATC TAGAGGATCC CGGGCTGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
            361 ATGACATCGA CTACAAGGAT GACGATGACA AGTAGTGATC CCGGGTGGCA TCCCTGTGAC
            421 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC
            481 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG
            541 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG
            601 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC
            661 TCCTGGGGGT TCAAGCGATT CTCCTGCCTC AGCCCTCCCC GAGTTGGTTG GGATTCCAGG
            721 CATGCATGAC CCAGGCTCAG CTTAATTTTT GTTTTTTTTT GGTAGAGACG GGGTTTCACT
            781 CATAATTTGG CCAGGCTGGT CCCCAACTCC CAAATCTCAG GGGATCTACC ACCTTGGCCC
            841 TCCCAAATTG GGTGGGGATT AACAGGCGTT GAACCCCCTG GCTCCCCTTT CCCTGGTCCT
          901 TCTGAATTTT AAAAATTA
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB08520_A8Atb.seq check
          >08520_08520_A8At_2_hGHpolyA-R_C02_08_ABI24.ab1
              1 GCTAACCCCC CTCACCCCAT ATATTATAGA AGGACACCTA GTCAGACAAA ATGATGCAAC
             61 TTAATTTTAT TAGGACAAGG CTGGTGGGCA CTGGAGTGGC AACTTCCAGG GCCAGGAGAG
            121 GCACTGGGGA GGGGTCACAG GGATGCCACC CGGGATCACT ACTTGTCATC GTCATCCTTG
            181 TAGTCGATGT CATGATCTTT ATAATCACCG TCATGGTCTT TGTAGTCAGC CCGGGATCCT
            241 CTAGATTCTC CAGGAGGCTG CATCTGTTTA CAGAACTCAT CAAACTGCTG CTGCTCCAGT
            301 TCTGTCATGA CTCTGTTGAG GGCATAGATC TCTGCTCTTT GTCTCTGCTT CAGCTCCTGC
            361 TGGGCTGATT TCTGTTTGGC CTTCTCAACT CTGCTGACAG ATTCTTTAAC TTTGGTTTTT
            421 TCTTTACGTG CAGGTGGATC CATGGTGGCG GCCGCAAGCT TAATTCTGAC GGTTCACTAA
            481 ACGAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAACGG
            541 GGCGGGGTTA TTACGACATT TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC
            601 ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC
            661 ATTGGTGTAC TGCCAAAACC GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA
            721 CTGCCAAGTA GGAAAGTCCC GTAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT
            781 ACCGTCATTG ACGTCAATAG GGGGCGGACT TGGCATATGA TACACTTGAT GTACTGCCAA
            841 GTGGGCAGTT TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT
            901 TACTATGGGA ACATACGTCA TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC
            961 CAGGCGGGCC ATTACCGGTA AGTTATGTAA CGGCGGAACT CCATATATGG GCTATTGAAC
           1021 TAATGACCCC CGTAATTGAA TTACTATTAA TAACCTAGTT CAATTATCAT GGTCAACATG
           1081 CGTTCATAGT GGACAATGGA GCAATATAAT GGTACTATTA TGATATGAGA ATTACTACAA
          1141 CGT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Suzuki, Y., Isolation of a small vasohibin-binding protein (SVBP) and its role in vasohibin secretion. J. Cell. Sci. 123, 3094-4101, (2010). PMID 20736312. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl