Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-CMV-mRFP1-RfA

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.

Catalog number RDB08492
Resource name CSII-CMV-mRFP1-RfA
Clone info. Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by CMV promoter.
Comment Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging. Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB08492zzk02.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB08492.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08492 CSII-CMV-mRFP1-RfA DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CMV-mRFP1-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08492).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12133_A2E1p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: mCherry-check_R (Pr0521)
            Region: mRFP1 5', CMV pro_2, CMV en_2
            Sequence file: RDB12133_A2E1a.seq check
            >08492_12133_B1K2F_mCherry_check_R_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNCNNCCNC NNTCCTCNNA GTTCNTCACG CGCTCCCACT TGAAGCCCTC
               61 GGGGAAGGAC AGCTTCAAGT AGTCGGGGAT GTCGGCGGGG TGCTTCACGT AGGCCTTGGA
              121 GCCGTACTGG AACTGAGGGG ACAGGATGTC CCAGGCGAAG GGCAGGGGGC CGCCCTTGGT
              181 CACCTTCAGC TTGGCGGTCT GGGTGCCCTC GTAGGGGCGG CCCTCGCCCT CGCCCTCGAT
              241 CTCGAACTCG TGGCCGTTCA CGGAGCCCTC CATGCGCACC TTGAAGCGCA TGAACTCCTT
              301 GATGACGTCC TCGGAGGAGG CCATCGGATC CAGTCGAGAC CGGTAGCGCT AGCGGATCTG
              361 ACGGTTCACT AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT
              421 TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA
              481 AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG
              541 CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA
              601 TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAANGNC ATGTACTGGG CATAATGNCN
              661 GGCGGGCNNT TTACNGTCNT TGACGTCNAN AGGGGGNGTA CTTGGNATAN GANACNNNNN
            721 NNGNNCNNNC NNNNNNGGNN NNNTTNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE, mRFP1 3'
            Sequence file: RDB12133_A2E1b.seq check
            >08492_12133_B1K2F_PRE_rev_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNGNN NNNNNNCAAC ATAGTTAAGA ATACCAGTCA ATCTTTCACA
               61 AATTTTGTAA TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG
              121 GATCCGCGGC CGCGAATTCT TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT
              181 GTTCCACGAT GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT
              241 AGGCGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT
              301 AGTGGCCGCC GTCCTTCAGC TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT
              361 CGGGGTACAT CCGCTCGGTG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC
              421 CGTCGGAGGG GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA
              481 GGGAGGAGTC CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC
              541 ACTTGAAGCC CTCGGGGAAG GACAGCTTCA AGTAGTCGGG GANGTCGGCG GGGTGCTTCA
              601 CGTANGCCTT GNAGCCGTAC TGGAACTGAN GGGACAGGAN GTCCCAGGCN AANGGGNAGG
              661 GGGCCGCCCT TGNTCACCTT NNGCTTNNNN GTCTGGGNGN CNTNNNNNNG NNGNNCCTCG
            721 CCCTCNNCCN NNNNNTCNNN NNNNNNN
            //
            Primer: Cm_N_rev (Pr0014)
            Region: CmR, attR1, PRE
            Sequence file: RDB12133_A2E1c.seq check
            >08492_12133_B1K2F_Cm_N_rev_C01_03_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTGG GNNNTATCAA CGGTGGTATA TCCNAGTGAT
               61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT
              121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC
              181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC
              241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA
              301 CTTGTGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT
              361 AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT
              421 GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG
              481 GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA
              541 TGCGGGGAGG CGGCCCAAAG GGAGATCCGA CTCGTCTGAN GGCGAANGCG AAGACGCGGA
              601 ANANGCCGCA NANCCGGCAG CANGCCGCGG GGAAGGAAGG TCCGCTGGAN TGANGGCCNA
              661 NNGGACGTAN CANANNNNNG TCCCNCGCAN AATCCNNNTG GCNNCNCNNN CNANNANNCN
            721 NNGAANNNNN NNNNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: bGH pA, 3'LTR, attR2, ccdB
            Sequence file: RDB12133_A2E1d.seq check
            >08492_12133_B1K2F_bGH_rev3_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTAAACGG
               61 GCCCTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA GTTACCAGAG
              121 TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA TTGAGGCTTA
              181 AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC TAACCAGAGA
              241 GACCCAGTAC AGTCCGGATC ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG
              301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
              361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT
              421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG
              481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC
              541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGNNTTTA
              601 TCTGACAGCA GACGTGCACT GGCCNGGGGG NNCANCATCC GTCNCCNGGG NGTGTCNATA
              661 ATATCACTCT GNNCATCCNN NAANNGACNA NNNNNGNTCT CNCNTTTNNN NNNGGNNNNN
            721 ANNNTNNANN NNNNNNTTNN NNNNNNNN
            //
            Primer: HIV-B_R (Pr0673)
            Region: HIV-1 psi, 5'LTR, CMV pro_1, CMV en_1
            Sequence file: RDB12133_A2E1e.seq check
            >08492_12133_B1K2F_HIV-B_R_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCANC NAGCCGAGTC
               61 CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC
              121 TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC
              181 ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA
              241 GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC
              301 CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT
              361 TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA
              421 AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC
              481 TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT
              541 ACGTAGATGT ACTGCCAAGT ANGAAAGTCC CATAANGTCA TGTACTGGGC ATAATGNCAG
              601 GCGGGCCATT TACCGTCATT GACGTCNATA NGGGGCGTAC TTGNCATATG ANACACTNNA
            661 NGNACTGNNN NNNNNNN
            //
            Primer: cPPT/CTS-E_R (Pr0725)
            Region: cPPT_CTS
            Sequence file: RDB12133_A2E1f.seq check
            >08492_12133_B1K2F_cPPT_CTS-E_R_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNTATT ANNTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC
               61 CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT CACTAATCGA
              121 ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT
              181 CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG CCTAACTCTA
              241 TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCATT
              301 ATGAATAATT TTATATACCA CAGCCAATTT GTTATGTTAA ACCAATTCCA CAAACTTGCC
              361 CATTTATCTA ATTCCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG CGATTCTTCA
              421 ATTAAGGAGT GTATTAAGCT TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC
              481 GTGTGATTCC AAATCTGTTC CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA
              541 GTGGTGCAAA TGAGTTTTCC AGAGCAACCC CNAATCCCCA GGANCTGTTG ATCCTTTANG
              601 TATCTTTCCN CAGCCAGGAN TCTTGCCTGG ANCTGCTTGA TGCCCCAGAC TGNGANTTGC
              661 NNNNNANGCT GNTNNGCCTC NNNANCCCTN NNCNANNNGN TCTGCTGCTG NNNNNNNNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori, Zeo
            Sequence file: RDB12133_A2E1g.seq check
            >08492_12133_B1K2F_SV40pro_ori_F_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNGNNN TNNTCNNGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
               61 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT
              121 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA
              181 ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG
              241 GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC
              301 GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG
              361 GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG
              421 GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG
              481 CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG
              541 GCCGANGAGC AGGACTGACA CGTGCTACGA GATTTCGATT CCNCCGCCGC CTTCTATGAA
              601 AGGNTGGGCT TCNGANNCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCN GCGCGGGGNT
              661 CTCNTGCTGG AGNNCNTCNC CNNCCCCNNC NNNNTNANNG NNNCTTNNNN NNNNTNNNNN
            721 NNANNNNNNN NNN
            //
            Primer: RRE_F1 (Pr0796)
            Region: RRE
            Sequence file: RDB12133_A2E1h.seq check
            >08492_12133_B1K2F_RRE_F1_H01_08_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNTTGNCTG NNGGCTATTG
               61 NNGCNNNNNN NTCTGTTGCA ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC
              121 CTGGCTGTGG AAAGATACCT AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA
              181 AAACTCATTT GCACCACTGC TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA
              241 CAGATTTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC
              301 TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA
              361 TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG
              421 TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT
              481 GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCCAC
              541 CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAANG TGGAGAGAGA
              601 GACAGAGACA GATCCNTTCG ATTAGTGAAC GGATCTACAA ATGGCAGTAT TCNTCCNCAA
              661 TTNNAAAAGA AAANGGGGGN NNGGGGGGGN ACAGTGCNGG GNNAANNATA GTAGANNTNN
            721 NAGCNNNNGA NNTNCNANCT NAANNNNNNN NAAANNNANN NNNNAAAANN NNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.05.01

            GNP_filter3_RDBDEP_html_230501.pl