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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMx-E4BP4 (Nfil3)

Expression clone of mouse Nfil3 cDNA

Catalog number RDB08379
Resource name pMx-E4BP4 (Nfil3)
Alternative name Nfil3(E4BP4)/pMx-IRES-GFP
Clone info. Expression clone of mouse Nfil3 cDNA (NM_017373).
Vector backbone pMX-IRES-GFP (Plasmid)
Size of vector backbone 5.9 Kb
Selectable markers Amp^r
Gene/insert name Mouse Nfil3 cDNA
Depositor|Developer Taniguchi, Masaru |
Other clones in our bank
External Database
Mouse Nfil3
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested(Nature Immunology 12(5), 450-459, 2011).
          Additional terms and conditions:
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nature Immunology 12(5), 450-459, 2011)。
          付加的提供条件：
          pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08379 pMx-E4BP4 (Nfil3) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Motomura, Y., The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells. Nature Immunology 12(5), 450-459, (2011). PMID 21460847.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08379_A5Bc.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB08379_A5Bca.seq check
          >08379_08379_A5Bc_pBABE5'_D11_11.ab1
              1 AGGGAAATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGCTAA ACTTCCCTGA
             61 CCCTGACATG ACAAGAGTTA CTAACAGCCC CTCTCTCCAA GCTCACTTAC AGGCTCTCTA
            121 CTTAGTCCAG CACGAAGTCT GGAGACCTCT GGCGGCAGCC TACCAAGAAC AACTGGACCG
            181 ACCGGTGGTA CCTCACCCTT ACCGAGTCGG CGACACAGTG TGGGTCCGCC GACACCAGAC
            241 TAAGAACCTA GAACCTCGCT GGAAAGGACC TTACACAGTC CTGCTGACCA CCCCCACCGC
            301 CCTCAAAGTA GACGGCATCG CAGCTTGGAT ACACGCCGCC CACGTGAAGG CTGCCGACCC
            361 CGGGGGTGGA CCATCCTCTA GACTGCCGGA TCCACTAGTA ACGGCCGCCA GTGTGCTGGA
            421 ATTCGCCCTT CTGATGCAGC TGAGAAAAAT GCAGACCATC AAAAAGGAGC CCGCACCCCT
            481 AGATCCTACC AGCAGCTCAG ACAAGATGCT GCTGCTGAAC TCTGCCTTAG CTGAGGTGGC
            541 CGAGGACCTA GCCTCAGGTG AAGATTTGCT CCTGAACGAA GGGAGCATGG GGAAAAACAA
            601 ATCCTCGGCG TGTCGGAGAA AACGGGAATT CATTCCGGAC GAGAAGAAAG ACGCCATGTA
            661 TTGGGAGAAA CGGCGGAAAA ACAACGAAGC TGCCAAAAGA TCTCGGGAGA AGCGCCGCCT
            721 CAATGACCTG GTTTTGGAGA ACAAGCTGAT CGCCCTGGGA GAAGAAAATG CCACTTTAAA
            781 AGCTGAGCTG CTCTCCCTGA AATTAAAGTT TGGTTTAATT AGCTCCACGG CGTATGCCCA
            841 AGAAATCCAG AAACTCAGTA ATTCCACAGC TGTCTACTTT CAGGACTACC AGACATCCAA
            901 GGCTGCCGTG AGCTCTTTTG TGGACGAGCA TGAGCCTGCG ATGGTAGCCG GAAGTTGCAT
            961 CTCAGTCATC AAGCACTCTC CCCAGAGCTC GCTCTCCGAT GTGTCAGAGG TGTCCTCGGG
           1021 TGGGAGCACA CTCAGAAAGC CCCCGCACAG GGGAGGGCTG GCCGGAGCCT GAGACAGTTC
          1081 CGTGTGATCA AGCAGAAGCC GTGTAGATGG GAGAGCTTTT GGCCAGGGAA A
          //
          Primer: IRES-R
          Sequence file: RDB08379_A5Bcb.seq check
          >08379_08379_A5Bc_IRES-R_E11_14.ab1
              1 GCCCCGCGGC TCGGCAGTAC GTTAGGGGGG GGGGAGGGAG AGGGGGGCGG GGGGGCGGAA
             61 TTTACGTAGC GGCCGCCAGG GGGATGGATA TCTGCAGAAT TCGCCCTTGC AGCCATTTAC
            121 CTGGAGTCCG AAGCCGAGAT CGGTTGTGTG GCTATGAATC TCTTGAGCGA GACCACCTCT
            181 GCAGATAAGT TTGCTATTCC CTGCTTCAAA TACAAATTCT CAGCTTCGGA AACCTTATAG
            241 CCACCGTCTT TGACTTCCAC CACACCTGTT TTGAAGCTAC TCTGAGTTTT GCTGCTCAGT
            301 TCTTTGTGAT GCCAGTGTTC CGATTTGAGG GACCAATCTT GAATGTTCGT CACCTGCACT
            361 GAGAAAGGAG GGAGGGAGGA ATGGGCCATA CCCGAGGTTC CATGTTTCTC CAGGTCAAAA
            421 TGTCTTTTGG CGATCGCATC GGCGGGTGAA GAGAGTTTCT GCATGCCTTC AAACTCGCTG
            481 TCCAAAGCCT CCACTTTGAC CTGCATGGCC TTGGCTTTAA TCCGAAGCTT GTGCGGTAAG
            541 GCAGAAGGGT TCACTTCCGG AACCTTCACC ACCGTGGCGT GGACCCGTTG CAGCTCCACT
            601 GGAGAATGGA TGGGGCCCTT AGGGACCTGT TGTTCGTCTT CCCCATCAGA AGACTTGCCC
            661 ACTACACCCT CATCGGCCTC TGAGGTTCTT GGGGAGTTGC TAGTGGACCC ATGGACCTGC
            721 AAGAGGGGTG GGGAGTGGGA GTAAGTGGAG AAAGAGCTTC CCATGTAGCT CTGGTAGATG
            781 GAGGTGGAAT ACGTGCCCCG CTCCTCCCTG GCCTCCCTGG CAAAGCTCTC CAACTCCACG
            841 GGCTCCTGCT TGATCACAGG GAACTTGTTC TCAGGGCTCC GGCAGCCTCC CTGTGCGGGG
            901 CTTTCCTGAG TGTGCTCCAC CGAGGACACC TCTGACACAT CGGAGAGCGA GCTCTGGGGA
            961 GAGTGCTTGA TGACTGAGAT GCAACTTCCG GCTACCATCG CAGGCTCATG CTCGTCCCAC
           1021 AAAAAGAGCT CACGGCCAGC CCTTTGGATG TCCTGGGTAG TTCCCTGGAA AGGTTAGAAC
           1081 AGCTGTGGAA TTACCTGAGT TCCTGATTTC GTGGCCAATA CGCCGGTGGA AAGCTTATTT
          1141 AGGC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Motomura, Y., The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4+ T cells. Nature Immunology 12(5), 450-459, (2011). PMID 21460847.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl