Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET21c_Tr-egl1-His

Expression vector of Endoglucanase EG-I of Trichoderma reesei

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08288_A6G4p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB08288_A6G4a.seq
>08288_08288_A6G4_T7_20bp_A07_01.ab1     1 TGCTCATTCC ATCTAGAATA ATTTTGTTTA ACTTTAAGAA GGAGATATAC ATATGGCTAG    61 CATGACTGGT GGACAGCAAA TGGGTCGGAT CCGAATTCGA GCTCCGTCGA CCCAGCAACC   121 GGGTACCAGC ACCCCCGAGG TCCATCCCAA GTTGACAACC TACAAGTGTA CAAAGTCCGG   181 GGGGTGCGTG GCCCAGGACA CCTCGGTGGT CCTTGACTGG AACTACCGCT GGATGCACGA   241 CGCAAACTAC AACTCGTGCA CCGTCAACGG CGGCGTCAAC ACCACGCTCT GCCCTGACGA   301 GGCGACCTGT GGCAAGAACT GCTTCATCGA GGGCGTCGAC TACGCCGCCT CGGGCGTCAC   361 GACCTCGGGC AGCAGCCTCA CCATGAACCA GTACATGCCC AGCAGCTCTG GCGGCTACAG   421 CAGCGTCTCT CCTCGGCTGT ATCTCCTGGA CTCTGACGGT GAGTACGTGA TGCTGAAGCT   481 CAACGGCCAG GAGCTGAGCT TCGACGTCGA CCTCTCTGCT CTGCCGTGTG GAGAGAACGG   541 CTCGCTCTAC CTGTCTCAGA TGGACGAGAA CGGGGGCGCC AACCAGTATA ACACGGCCGG   601 TGCCAACTAC GGGAGCGGCT ACTGCGATGC TCAGTGCCCC GTCCAGACAT GGAGGAACGG   661 CACCCTCAAC ACTAGCCACC AGGGCTTCTG CTGCAACGAG ATGGATATCC TGGAGGGCAA   721 CTCGAGGGCG AATGCCTTGA CCCCTCACTC TTGCACGGCC ACGGCCTGCG ACTCTGCCGG   781 TTGCGGCTTC AACCCCTATG GCAGCGGCTA CAAAAGCTAC TACGGCCCCG GAGATACCGT   841 TGACACCTCC AAGACCTTCA CCATCATCAC CCAGTTCAAC ACGGACAACG GCTCGCCCTC   901 GGGCAACCTT GTGAGCATCA CCCGCAAGTA CCAGCAAAAC GGCGTCGACA TCCCCAGCGC   961 CCAGCCCGGG CGGCGACACC ATCTCGTCCC TGCCCGTCCC GCCTCAGCCT ACGGCGGGCC  1021 TCGCCACCCA TGGGCAACGC CCCTGAGCAG CGGGCATGGT GCTTCGTTGA TCAGCATTTG  1081 CACGACACAG GCAGTACATG GACCTGGCCT TCGACAGGCG GGCCAACCGC CCGGACCCCC 1141 CTGCA //
Primer: f1ori_R3 Sequence file: RDB08288_A6G4b.seq
>08288_08288_A6G4_f1ori_R3_E07_13.ab1     1 GCATGGCATC GGATTAGTTG CTCCTTTCAG CAGAAACCCC TCAAGACCCG TTTAGAGGCC    61 CCAAGGGGTT ATGCTAGTTA TTGCTCAGCG GTGGCAGCAG CCAACTCAGC TTCCTTTCGG   121 GCTTTGTTAG CAGCCGGATC TCAGTGGTGG TGGTGGTGGT GCTCGAGTGC GGCCGCAAGC   181 TTGTCGACAA GGCATTGCGA GTAGTAGTCG TTGCTATACT GGCACGTAGT GCCCGACGTG   241 CACGTCTTGC ACCCGCTGTA CCCAATGCCA CCGCACTGCC CCCAGTGAGT CTGCGTGCAG   301 CTCGGGCTGC TCGAAGTCGT CGAGCTCCTC CGTGTAGTCG AAAACGTCGT GCTGGACGCA   361 GGCGGGGGCG GGGGCGCAGT CGAGTTCGTA GTAGACCCAA TGTCTCCCCA GCGGATGTTG   421 GAGAAGACGA CGTGCGTGTT GGGGTTGTTG GCCAGGATGT TGGATGGGTT GCCCTCGGTG   481 CTGCTGCAGG GGCCGGCGTT GCCGCTGTCG AGCCAGTTCA TGTACTGGCT GTTGTCGTTC   541 CAAATGCTGA ACACGAGCAC CATGCCGCTG CTCAGGGCCT TGCCCATGGT GGCGAGGCCG   601 CCGTAGGCTG AGGCGGACGG GCAGGACGAG ATGGTGTCGC CGCCGGGCTG GGCGCTGGGG   661 ATGTCGACGC CGTTTTGCTG GTACTTGCGG GTGATGCTCA CAAGGTTGCC CGAGGGCGAG   721 CCGTTGTCCG TGTTGAACTG GGTGATGATG GTGAAGGTCT TGGAGGTGTC AACGGTATCT   781 CCGGGGCCGT AGTAGCTTTT GTAGCCGCTG CCATAGGGGT TGAAGCCGCA ACCGGCAGAG   841 TCGCAGGCCG TGGCCGTGCA AGAGTGAGGG GTCAAGGCAT TCGCCCTCGA GTTGCCCTCC   901 AGGATATCCA TCTCGTTGCA GCAGAAGCCC TGGTGGCTAG TGTTGAGGGT GCCGTTCCTC   961 CATGTCTGGA CGGGGCACTG AGCATCGCAG TAGCCGCTCC CGTAGTTTGG CACCGGCCGT  1021 GTTATACTGG GTTTGGCGCC CCCGTTCTCG TCCCATCTGA GACAGTAGAG CGAGCCCGAT  1081 TCTTCTCCAC ACGCCAGAGG CAGAGAAGGT CGACGATCGA AGGCCTCCAG CCTTCCTTGG 1141 CCGT //
Primer: pGL3_RV4 Sequence file: RDB08288_A6G4c.seq
>08288_08288_A6G4_pGL3_RV4_A08_02.ab1     1 GTAACTATGG GTTGAGCTCT CAGGGCATCG GTCGAGATCC CGGTGCCTAA TGAGTGAGCT    61 AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC   121 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCCAGG   181 GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC   241 TGAGAGAGTT GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG   301 ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC   361 GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC   421 ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG CATTTGCATG   481 GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT   541 TGATTGCGAG TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT   601 AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC   661 AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA   721 TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC ATCCTGGTCA   781 TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC   841 GCTTTACAGG CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT   901 TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG   961 GAGGTGGCAA CGCCCAATCA GCAACGACTG TTTGCCCGCC CAGTTTGTTG TGCCACGCGG  1021 GTTTGGGAAT GTAAATTTCA GCTCGCCATC GCGCTTCCAC TTTTTCCCGG CGGTTTTCGC  1081 AGAAACGTTG GCCTACTGGC TCAACCAACG CGGGCAAACG GATCTGAATA AGGAGACCAC 1141 CCGGGCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl