Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-UbC-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by UbC promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12132_A9Cip1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: UbCpro_F () Sequence file: RDB12132_A9Cia.seq
>08255_12132_A9Ci_UbCpro_F_A04_01_ABI08.ab1     1 AGACATTTGA AAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC TGGCCGTTTT    61 TGGCTTTTTT GTTAGACCGG ACCGTGTTTA GCGCTACCGG TCTCGAGAAT TCTGCAGCGG   121 CCATCACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA   181 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC   241 AGTCACTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA   301 TAATGTGTGG ATTTTGAGTT AGGATCCGTC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT   361 GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA   421 TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT   481 TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA   541 CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA   601 GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC   661 GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC   721 GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA   781 TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC   841 CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA   901 CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTTTGTGATG GCTTCCATGT   961 CGGCAGGAAT GCTTTAATGA AATTACAACA GTACTGCGAT GAGTGGCCAG GGCGGGGGCG  1021 TAAAACGCGT GGGATCCGGC TTACTAAAAA GCCCAGAATA ACAGTATGCG GTAATTTGCG  1081 CGCTGATTTT TTGGCGGTAT AGGAAATATA ATACTTGAAT ATGTATACCG AAAGTATGTC 1141 AAAAGAGGTA TGCTTATTGA AGCCAAGCCG TTATTTACCA GTGGAACACG G //
Primer: Cm_C_F () Sequence file: RDB12132_A9Cib.seq
>08255_12132_A9Ci_Cm_C_F_B04_02_ABI08.ab1     1 CAAGAACGCG GTGGGACCGG CTTACTAAAA GCCAGATAAC AGTATGCGTA TTTGCGCGCT    61 GATTTTTGCG GTATAAGAAT ATATACTGAT ATGTATACCC GAAGTATGTC AAAAAGAGGT   121 ATGCTATGAA GCAGCGTATT ACAGTGACAG TTGACAGCGA CAGCTATCAG TTGCTCAAGG   181 CATATATGAT GTCAATATCT CCGGTCTGGT AAGCACAACC ATGCAGAATG AAGCCCGTCG   241 TCTGCGTGCC GAACGCTGGA AAGCGGAAAA TCAGGAAGGG ATGGCTGAGG TCGCCCGGTT   301 TATTGAAATG AACGGCTCTT TTGCTGACGA GAACAGGGGC TGGTGAAATG CAGTTTAAGG   361 TTTACACCTA TAAAAGAGAG AGCCGTTATC GTCTGTTTGT GGATGTACAG AGTGATATTA   421 TTGACACGCC CGGGCGACGG ATGGTGATCC CCCTGGCCAG TGCACGTCTG CTGTCAGATA   481 AAGTCTCCCG TGAACTTTAC CCGGTGGTGC ATATCGGGGA TGAAAGCTGG CGCATGATGA   541 CCACCGATAT GGCCAGTGTG CCGGTCTCCG TTATCGGGGA AGAAGTGGCT GATCTCAGCC   601 ACCGCGAAAA TGACATCAAA AACGCCATTA ACCTGATGTT CTGGGGAATA TAAATGTCAG 661 GCTCCCTTAT ACACAGCCAG TCTGCAGGTC GACCATAGTG ACTGGA //
Primer: IRES-F () Sequence file: RDB12132_A9Cic.seq
>08255_12132_A9Ci_IRES-F_C04_03_ABI08.ab1     1 GGCAGTCCCT TTCATGGTGT TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA    61 CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA   121 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA   181 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA   241 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA   301 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT   361 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG   421 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA   481 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT   541 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG   601 CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC GACCACTACC   661 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT   721 ACCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT   781 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA GAATTCAGGG   841 CCTCTCGAGC CTCTAGAGTT AACATCGAGG GATCAAGCTT ATCGATAATC AACCCTCTGG   901 ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT   961 GTGGATACGC TGCTTTAATG CCTTTGGTAT CATGCTATTG CTTCCCGGTA TGGCTTTCAA  1021 TTTTTCTTCT CCTGTATAAA TCCCTGGGTT TGCCTGGTCT CTTATGAGGA GTTGTGGGCC  1081 CCGTTGTCAA GGCCAACCGT TGCGTGATGT GCAACTGTTG TTTGGCTGGA ACGCACCCCC 1141 ACTTGGTTGG GGGCATTTGA CCTACCAGCT TGTTTCTAAG CTTCCTTATC //
Primer: CMV-Forward () Sequence file: RDB12132_A9Cid.seq
>08255_12132_A9Ci_CMV-Forward_D04_04_ABI08.ab1     1 AGGGATCCAC CTTCCCGCCC CATTGACGGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG    61 TCTATATAAG CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC   121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA   181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA   241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA   301 AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA   361 GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG   421 AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA   481 AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA   541 GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA   601 GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT   661 TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA   721 AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG   781 CGGCCGCTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAAATTGGA GAAGTGAATT   841 ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAAGGAGT AGCACCCACC AAGGCAAAGA   901 GAAGAGTGGT GCAGAGAGAA AAAAGAGCAG TGGGGAATAG GAGCTTTTGT TTCCTTGGGT   961 TTCTTTGGGA GCAGCAGGGA AGCACTATGG GGCGGCAGCG TTCAATGGAC GCTGACGGTA  1021 CAAGGCCCAG AACAATTTAT TGTTCTGGTA TTAGTTGCAG CCAGCAGAAA CAAATTTTGC  1081 CTGGAAGGGG CTATATTTGG AAGGGCCGCA ACAGCATCCT GTTTGCAAAC TCTCAACAGT  1141 CTGGGGGCAT TCAAGCAGCT CCCAGGCCAA AGAAATTCCT TGGCCTTGTT GTAGGAAGAG 1201 ATATGATGCT //
Primer: BGH_rev2 () Sequence file: RDB12132_A9Cie.seq
>08255_12132_A9Ci_BGH_rev2_E04_05_ABI08.ab1     1 TTCGTAGGCG GGGTTCAGGA GCACGGGGGA GGGGCAAACA ACAGATGGCT GGCAACTAGA    61 AGGCACAGTC GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG   121 ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA   181 CTTGAAGCAC TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG   241 AGAGCTCCCA GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT   301 CTCGGGCCAT GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT   361 CCGGGTCATC CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT   421 TGGCTTCTTC TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA   481 GTGGATATCT GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG   541 TGTGGTAGAT CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA   601 GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC   661 TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC   721 AGGCACAATC AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA   781 GGTCTCGACG GTATCGATGC GGGGAGGCGG CCCAAAGGGA GATCCGACTC GTCTGAGGGC   841 GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG   901 CTGGATTGAG GGCCGAAAGG GACGTAGCAA AAAGGACGTC CCGCGCAGAA TCCAGGTGGC   961 AACACAGGGC GAGCAGCCAT GGGAAAGGAC GTCAGCTTCC CGACAACACC ACGGAAATTG  1021 TCAGTGCCCA ACAGCCGAGC CCCTGGTCCA GCAGCGGGGC CAAGGCAGCG GGCGATGAAG  1081 TTCCGCCGTG GGCAATAGGG AGGGAAAAGG GCGAAGGTTC CGAAGAGCTG GACCAGGTGT 1141 GCCAATGCCC CAACCAGTGG GGATTGCCGT CCAGACA //
Primer: SV40pro_F () Sequence file: RDB12132_A9Cif.seq
>08255_12132_A9Ci_SV40pro_F_F04_06_ABI08.ab1     1 CCTTCGGGGC CCCGGACATT CAGGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT    61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA   121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA   181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG   241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG   301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA   361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG   421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC   481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG   541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT   601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA   661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA   721 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT   781 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT   841 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC   901 ACAACATACG AGCCGGAAGC ATAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT   961 CACATTAATT GCGTTGCGCT CACTGCCCCG CTTTCCAGTC GGGAAAACCT GTCGTGCCAG  1021 CTGGCAATTA ATGAATTCGG GCCCAACGCG CCGGGGAGAG GCGGTTTGCG TAATTGGGGC  1081 GCTCTTTCGC TTCCTTCGCT CACTGAACTC GCTGCGGCTT CGGTCGCTCG GCTGCGGCGA  1141 GCGGGTATCA GGCCTCCACT CAAAGGGCCG GTTTAAATAC CGCGGTTTAA TTCTCCAACC 1201 A //
Primer: env_F () Sequence file: RDB12132_A9Cig.seq
>08255_12132_A9Ci_env_F_G04_07_ABI08.ab1     1 GCGCGGGGGC CGGGATCCAT TCGATTAGTG AACGGATCTA CAAATGGCAG TATTCATCCA    61 CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT   121 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT   181 TCGGGTTTAT TACAGGGACA GCAGAAATTC ACTTTGAATT CTAGCTCGAG ATCGGGAGAT   241 CTGGCCTCCG CGCCGGGTTT TGGCGCCCCC CGCGGGCGCC CCCCTCCTCA CGGCGAGCGC   301 TGCCACGTCA GACGAAGGGC GCAGCGAGCG TCCTGATCCT TCCGCCCGGA CGCTCAGGAC   361 AGCGGCCCGC TGCTCATAAG ACTCGGCCTT AGAACCCCAG TATCAGCAGA AGGACATTTT   421 AGGACGGGAC TTGGGTGACT CTAGGGCACT GGTTTTCTTT CCAGAGAGCG GAACAGGCGA   481 GGAAAAGTAG TCCCTTCTCG GCGATTCTGC GGAGGGATCT CCGTGGGGCG GTGAACGCCG   541 ATGATTATAT AAGGACGCGC CGGGTGTGGC ACAGCTAGTT CCGTCGCAGC CGGGATTTGG   601 GTCGCGGTTC TTGTTTGTGG ATCGCTGTGA TCGTCACTTG GTGAGTAGCG GGCTGCTGGG   661 CTGGCCGGGG CTTTCGTGGC CGCCGGGCCG CTCGGTGGGA CGGAAGCGTG TGGAGAGACC   721 GCCAAGGGCT GTAGTCTGGG TCCGCGAGCA AGGTTGCCCT GAACTGGGGG TTGGGGGGAG   781 CGCAGCAAAA TGGGCGGCTG TTCCCGAGTC TTGAATGGAA GACGCTTGTG AGGCGGGCTG   841 TGAGGTCGTT GAAACAAGGT GGGGGGCATG GTGGGCGGCA AGAACCCAAG GTCTTGAGCC   901 TTCGCTAATG CGGGGAAAGC TCTTATTCGG GTGAGATGGG CTGGGGGCAC CATCTGGGGA   961 CCCTGACGTG AAGCTTTGTC ACCTGACTGG AAAACTCGTT TGTCGTCTGT GCCGGGGGGC  1021 GGCAGTTAAT GGGCGTGCGT TGGGCAATGC CACCCGGTAA CCTTGGGGAA GCGCGCGGCC 1081 CTTCGGTCGT GGTCGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl