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CSII-CMV-RfA-IRES2-hKO1

Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.

Catalog number RDB08070
Resource name CSII-CMV-RfA-IRES2-hKO1
Clone info. Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with hKO1 fluorescent protein marker.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-CMV-RfA-IRES2-hKO1 ()
Selectable markers Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence RDB08070z.seq
            Remarks, protocol and/or map (pdf) RDB08070.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08070 CSII-CMV-RfA-IRES2-hKO1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CMV-RfA-IRES2-hKO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08070).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12148_A6Cfp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Cm_M_R
            Sequence file: RDB12148_A6Cfa.seq check
            >08070_12148_A6Cf_Cm-M_R_C09_09.ab1
                1 GCTGAACTAC CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAATTCCGG
               61 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
              121 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA
              181 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC
              241 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA
              301 CGGATCCTAA CTCAAAATCC ACACATTATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
              361 GGTGCCTAAT GCGGCCGCCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC
              421 TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT
              481 TCAGCTTTTT TGTACAAACT TGTGATGCTA GCGGATCTGA CGGTTCACTA AACCAGCTCT
              541 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT
              601 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC
              661 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA
              721 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT
              781 AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT
              841 GACGTCAATA GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT
              901 TACCGTAAAT ACTCCACCCA TTGACGTCAA TGGAAAAGTC CCTATTGGCG TTACTATGGG
              961 AACATACGTC ATTATTGACG TCATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGCCA
             1021 TTTACCGTAA GTTATGTAAC GCGGACTCAT ATATGGCTAT GACTATGACC CCGTATGGAT
            1081 ACTATATTCG ATAATCAGTG ATCTGCTGTC CCTGTATAAC CGAAATTTGG ATTTTTT
            //
            Primer: IRES-R
            Sequence file: RDB12148_A6Cfb.seq check
            >08070_12148_A6Cf_IRES-R_G09_21.ab1
                1 GCCCAAGGGG GCTCGGCCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG
               61 CCGCTGCAGA ATTCTCGAGA CCGGTAGCAT CACCACTTTG TACAAGAAAG CTGAACGAGA
              121 AACGTAAAAT GATATAAATA TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC
              181 ATAATACTGT AAAACACAAC ATATCCAGTC ACTATGGTCG ACCTGCAGAC TGGCTGTGTA
              241 TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT
              301 TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA
              361 TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC
              421 GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG
              481 GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT
              541 AGGTGTAAAC CTTAAACTGC ATTTCACCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC
              601 ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG
              661 GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA
              721 TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT
              781 TCATAGCATA CCTCTTTTTT GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC
              841 CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC TTTTAGTAAG CCCGGATCCA
              901 CGCGTTTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAAGCATTTC
              961 TGCCGACATG GAAAGCCCAT CCACAAACGG CCATGAATGA AACCTTGAAT TCGCCCAGGC
             1021 GGGCAATCAG CACCCTTTGT CGCCTTTGCG TATAATTATT TTGCCCCATG TGAAAACGGG
            1081 GGCCGAGAAG TTGTCATATT TGGGCTCACG TTTAAATTCC AAAACCTTGG T
            //
            Primer: IRES-F
            Sequence file: RDB12148_A6Cfc.seq check
            >08070_12148_A6Cf_IRES-F_F02_17.ab1
                1 CTCTAATGCC TGTTGCATGG TGTTTAGTCG AGGTTAAAAA CGTCTAAGGC CCCCCGGAAC
               61 CACGGGGACG TGGTTTTCCT TTGAAAAACA CGGATGATAA TATGGCCACG GGGATCCCCA
              121 CCATGGTGAG CGTGATCAAG CCCGAGATGA AGATGAAGTA CTTCATGGAC GGCAGCGTGA
              181 ACGGCCACGA GTTCACCGTG GAGGGCGAGG GCACCGGCAA GCCCTACGAG GGCCACCAGG
              241 AGATGACCCT GAGGGTGACA ATGGCCAAGG GCGGCCCCAT GCCCTTCAGC TTCGACCTGG
              301 TGAGCCACAC CTTCTGCTAC GGCCACAGGC CCTTCACCAA GTACCCCGAG GAGATCCCCG
              361 ACTACTTCAA GCAGGCCTTC CCCGAGGGCC TGAGCTGGGA GAGGAGCCTC CAGTTCAAGG
              421 ACGGCGGCTT CGCCGCCGTG AGCGCCCACA TCAGCCTGAG GGGCAACTGC TTCGAGCACA
              481 AGAGCAAGTT CGTGGGCGTG AACTTCCCCG CCGACGGCCC CGTGATGCAG AACCAGAGCA
              541 GCGACTGGGA GCCCAGCACC GAGAAGATCA CCACCTGCGA CGGCGTGCTG AAGGGCGACG
              601 TGACCATGTA CCTGAAGCTG GCCGGCGGCG GCAACCACAA GTGCCAGTTC AAGACCACCT
              661 ACAAGGCCGC CAAAGAAGAT CCTGAAGATG CCCCAGAGCC ACTTCATCGG CCACAGGCTG
              721 GTGAGGAAGA CCGAGGGCAA ACATCACCGA GCTGGTGGAG GACGCCGTGG CCCACTGCTG
              781 ACTGCAGGCT AGAGTTAACA TCGAGGGATC AAGCTTATCG ATAATCAACC CTCTGGATTA
              841 CAAAATTTGT GAAAGATTTG ACTGGTAATT TCTTAACTAT GTTGCTCCTT TTACGCTATG
              901 TGGATACGCT GCTTTTAATG CCTTTGTATC ATGCTATTTG CTTCCCGTTA TGGGCTTTTC
              961 ATTTTCTTCC TCCTTGTATA AATCCTGGGT TGCTGTCTCT TATGAAGGAG TTGTGGGCCC
             1021 GTTGTCAGGC AACGTTGGCG GTGGTTGTGC ACTTGTGTTT GCTGACGCAA CCCCCAACTG
             1081 GATTGGGGGC ATTTGGCCAA CGACCTTGTT CAGCTCCTTT TCCGGGGACT TTTCGCCTTT
             1141 TACCCCCATT CCTTATTTGC CCCAACGGCG AACTTCTATT CGCGTCGCCC TGTGACGTTG
            1201 CCGCATGCTT GTGACATCAA GGGGTGTTCT CTGA
            //
            Primer: HIVgp_R1
            Sequence file: RDB12148_A6Cfd.seq check
            >08070_12148_A6Cf_HIVgp_R1_A10_01.ab1
                1 CGGGCAATCC TAATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG
               61 GTGGGTGCTA CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC
              121 CAATTGTCCC TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG
              181 CGGTGGTCTT ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT
              241 TTATCTCTAT CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA
              301 GTTCTTCTGA TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT
              361 CTGATGTTTC TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA
              421 CTATATGTTT TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC
              481 GCGATCTAAT TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC
              541 CTCCGCTAGT CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC
              601 GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA
              661 AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA
              721 TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA
              781 GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT
              841 CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG ACCTCCCACC
              901 GTACACGCCT ACCGCCCATT TGCGTCAATG GGGGCGGGAG TTGTTACGAC ATTTTTGGAA
              961 AGTCCCGTTT GATTTTGGGT GCCAAAACAA ACTCCCATTT GAACGTCAAT GGGGTGGAGA
             1021 CTTGGAAATT CCCGTGAGTC AAACCGCTAT CCACGCCAAT GGATGGTACT GCCAAACGGC
            1081 AATCATCAAT GTAATAGCGA TGGAACTAAT TACGGTTAGA AA
            //
            Primer: BGH_rev2
            Sequence file: RDB12148_A6Cfe.seq check
            >08070_12148_A6Cf_BGH_rev2_B10_04.ab1
                1 CCCGGGCCGT TAGTAAGCGC GGGGGAGGGG CAGCAACAGA TGGCTGGCAA CTAGAAGGCA
               61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG GCCCTGCTAG AGATTTTCCA CACTGACTAA
              121 AAGGGTCTGA GGGATCTCTA GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA
              181 AGCACTCAAG GCAAGCTTTA TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC
              241 TCCCAGGCTC AGATCTGGTC TAACCAGAGA GACCCAGTAC AGTCCGGATG CAGCTCTCGG
              301 GCCATGTGAT GAAATGCTAG GCGGCTGTCA AACCTCCACT CTAATACTTC TCTCTCCGGG
              361 TCATCCATCC CATGCAGGCT CACAGGGTGT AACAAGCGGG TGTTCTCTCC TTCATTGGCT
              421 TCTTCTACCT TCTCTTGCTC AACTGGTACT AGCTTGTAGC ACCATCCAAA GGTCAGTGGA
              481 TATCTGATCC CTGGCCCTGG TGTGTAGTTC TGCCAATCAG GGAAGTAGCC TTGTGTGTGG
              541 TAGATCCACA GATCAAGGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
              601 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
              661 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
              721 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
              781 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
              841 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
              901 TTGAGGGCCG AAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAAATCCAG GTGGCAACAC
              961 AGGCGAGCAG CCCATGGGAA GGACGTCAGC TTTCCCTCGA CACACCACGG GAATTGTCAG
             1021 TGCCTCAACA GCCCGAGCCC CTGTCCAGCA GCGGGCAGCA GCGGCGATGA GTTCGGTCGT
            1081 GACCATTAGG ATGGGGGAAA GCTGTAAAGA TC
            //
            Primer: SV40pro_F
            Sequence file: RDB12148_A6Cff.seq check
            >08070_12148_A6Cf_SV40pro_F_C10_07.ab1
                1 CCCTGGTTGG TGGAACAAAT TCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
               61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA
              121 ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA
              181 TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG
              241 AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG
              301 TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA
              361 ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG
              421 TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC
              481 CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG
              541 AGGAGCAGGA CTGACACGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
              601 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
              661 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAAATAA
              721 AGCAATAGCA TCACAAATTT CACAAATAAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
              781 TTTGTCCAAA ACCTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG
              841 AGCTTGGGCG TAATCATGGT CAATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
              901 ATTCCACACA AACATACAAG CCGGAAAGCA ATAAAAGTGT TAAAAGCCTG GGGGTGCCCT
              961 AATGAGTGAG CTAAACTCAC ATTAAATTGC GTTTGCGCTT CACTGCCCCG CTTTCCAGTC
             1021 GGGAAAACCC TGGTTCGTGC CAAGCTTGCC ATTAATGGAA TTCGGGCCCA AACGGCGCGG
             1081 GGGAAGAGGC GGATTATGCC GTAATGGGGG GCCTTCTTCC GGCATTTCCT CCGCCTTCCA
            1141 CATTAAACT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            reference Iwasawa, C., Increased Cytotoxicity of Herpes Simplex Virus Thymidine Kinase Expression in Human Induced Pluripotent Stem Cells. Int. J. Mol. Sci. 20 (4). pii: E810 (2019). PMID 30769780.

            2023.11.16

            GNP_filter3_RDBDEP_html_231027.pl