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pcDNA3.1-His-hGLI3

Expression vector of human Gli3 cDNA

Catalog number RDB08066
Resource name pcDNA3.1-His-hGLI3
Clone info. Expression vector of human Gli3 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Selectable markers Amp^r
Gene/insert name Human GLI3 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Human GLI3

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08066 pcDNA3.1-His-hGLI3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1-His-hGLI3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08066).

          Reference section:

          Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18391_B0I7p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,6X His,T7 tag,Xpress tag,insert 5'
          Sequence file: RDB18391_B0I7a.seq check
          >08066_18391_B0I7_3_CMV_Forward_F02_17_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTANGCGTGT ACGGNGNGNA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTACC ATGGGGGGTT
            181 CTCATCATCA TCATCATCAT GGTATGGCTA GCATGACTGG TGGACAGCAA ATGGGTCGGG
            241 ATCTGTACGA CGATGACGAT AAGGTACCTA AGGATCCAGT GTGGTGGAAT TCTGCAGATG
            301 AAGACATCAT GGAGGCCCAG TCCCACAGCT CCACGACCAC TGAAAAGAAA AAAGTTGAGA
            361 ATTCCATAGT GAAGTGCTCC ACTCGAACAG ATGTGAGCGA GAAAGCCGTT GCCTCCAGCA
            421 CCACTTCTAA TGAGGATGAA AGTCCTGGAC AGACTTATCA CAGAGAGAGA AGAAACGCAA
            481 TCACTATGCA GCCACAGAAT GTCCAGGGGC TCAGCAAAGT CAGTGAGGAA CCTTCAACAT
            541 CGAGTGACGA GAGGGCCTCA TTGATCAAGA AAGAGATCCA TGGGTCCCTG CCACACGTGG
            601 CGGANCCCTC TGTGCCGTAC CGCGGGACGG TGTTTGCCAT GGACCCCAGG AATGGTTACA
            661 TGGAGCCCCA CTACCACCCT CCTCATCTTT TCCCTGCCTT CCATCCTCCT GTACCAATTG
            721 ATGCCAGACA TCATGANGGC CGTTACCATT ACGATCCATC TCCGATTCCT CCATTGCATA
            781 TGACTTCCGC CTTATCTAGT ANCCCTACGT ATCCGGACCT GCCCTTCATT ANGATCTCCC
            841 CACACCGGAN NCCNACNGCN GCTTCCNANN CNCCCTTCAG CCCTNCNCAT NCCNACATNA
          901 ATCCNTACAT GGNNNATATN NNNNNCNNNC ACANCAGCCN NTCGNNNTNN NNNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB18391_B0I7b.seq check
          >08066_18391_B0I7_3_BGH_rev3_G02_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNNGGNNNNN NACAGNNGGC TGGCAACTAG AAGGCACAGT
             61 CGAGGCTGAT CAGCGGGTTT AAACGGGCCC TCTAGACTCG AGCGGCCGCT CAACTCCTAT
            121 TGATTTCCGT TGGTTGCAGT CTTTTTTTCC TAAAGCCTAT TGCATAACTG CAAGGAATTT
            181 GCTTTCTTCC GCTAGGGAGG TCAGCAAAGA ACTCATGTCC CCGATAGCCA TGTTGGTGGT
            241 GCTCATGGAC AGCGCTGGGA ATGGGAGGGA CGCCCGAGGC GTGGTGAGGC GGGAGGAGCT
            301 ATGGGAAAGG TTCTGAATGA TACTTGGGCT CAGGGCCCCC GACATCAGGC TGGAGTGGTC
            361 CCCATCGTCT ATGATGGCAT CGAAGTCAAT CTGTACCCCT TCCAGGTCAT GGCTGTCGAG
            421 GCTGTCCACT GTGCTTGTCA CCTGATTAGC ACCTGGGGAA AGTAACTCAG AGTTTTTGGC
            481 GCTGGTCCCC TGTAGCAGGC AGCTGGCGTC TGAAATAGAG AATGAACCAG CTTTCGTGTC
            541 TTGCTGACTG AAGCCCACGG TTTGGTCATA GAACTGACCA GAGTAATTCT GCAGATTAGA
            601 GCACAGCGGA TGGGGCTGCC CTTTCATCTC CATCTTGATA CCATTCACCC TGCAGGTCTG
            661 ACTTGTGTCA CTGAGCTGTC CTGACTGCAG AGCAAGGCTG TCCCTCGGCA TAGCCTGGCG
            721 CCTGCTGCCC CCAAAGCTGG CACATGGCTG GTAGCCCCTG ACAACTGCCA AGCTTGACGG
            781 CTGGCTGCNC ATGCCGTGAN CCNNNGGCAN GCANCTCTCT GGCCCTTGGT ANATGTTGAT
            841 GTGTGANGTA NCACTAATCT GNCNAAGCAT CNGCTNANCG GGGGCNGNCN NNCCCNGGNN
          901 NCNGCNNGCT NNNNNNNANN NNNTGGNNAN CNNNNNNNCC NNNNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18391_B0I7c.seq check
          >08066_18391_B0I7_3_SV40pro_ori_F_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNCCTCT GCCTCTGNNC TATTCNNGAA GTAGTGAGGA GGCTTTTTTG
             61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC
            121 AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC
            181 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
            241 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
            301 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
            361 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
            421 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
            481 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
            541 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
            601 TTGTCGATCA GGATGATCTG GACGAANANC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
            661 CCAGGCTCAA GGCGCGCATG CCCGACGGCG ANGATCTCGT CGTGACCCAT GGCGATGCCT
            721 GCTTGCCGAA TATCATGGTG GAANATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
            781 TGGGNGTGGC GGNNCGCTAT CANGNNNTAN CGTTGGCTAC CCGNGNTATT GCTGAAGANC
            841 TTGGCNGCGA ANNGGCNNNN CGCTTNNNCN NGCTTTACNG NATCGNNGCN NCNNNNTNNN
          901 ANNNNATCGN CNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
          user_report Patel, R., Novel GLI3 pathogenic variants in complex pre- and postaxial polysyndactyly and Greig cephalopolysyndactyly syndrome Am. J. Med. Genet. A 185 (1): 97-104 (2021). PMID 33058447.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl