RDB08065 - pcDNA3.1-His-Gli2

Resource data sheet
Prev.
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pcDNA3.1-His-Gli2

Expression vector of mouse Gli2 cDNA

Catalog number	RDB08065
Resource name	pcDNA3.1-His-Gli2
Clone info.	Expression vector of mouse Gli2 cDNA.
Vector backbone	pcDNA3.1/HisB (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Gli2 cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Other clones in our bank

mouse Gli2

External Database
Mouse Gli2

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

Ordering Please visit Information of Request for Distribution.[link]
Order form [Credit Card Payment] [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB08065	pcDNA3.1-His-Gli2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-Gli2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08065).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17729_A9Kfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5' Sequence file: RDB17729_A9Kfa.seq
>08065_17729_A9Kf_3_CMV_Forward_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGGGCG GTAGGCGNGN NNNGTGGGAG 61 GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA 121 ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTACCA 181 TGGGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA 241 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGTACCTAG GATCCAGTGT GGTGGAATTC 301 TGCAGATCTC TGAGATGGAG ACTTCTGCCC CAGCCCCTGC ACTGGAGAAG AAAGAAGCCA 361 AGAGTGGTCT CTTGGAGGAC AGCAGCTTCC CCGACCCAGG GAAAAAGGCC TGTCCTCTGG 421 CGGTGGCCGC AGCTGTAGCC GCCCACGGAG TTCCTCAGCA GCTCCTGCCG GCTTTCCACG 481 CGCCTTTGCC GATTGACATG AGACACCAGG AGGGAAGGTA CCATTATGAC CCTCACTCTG 541 TCCACAGTGT ACACGGGCCT CCCACCCTAA GTGGCAGCCC TGTCATCTCA GATATCTCCT 601 TGATACGACT TTCTCCACAC CCTGCTGGCC CTGGAGAGTC ACCCTTCAGC GCCCACCACC 661 CCTACGTGAA CCCCCATATG GAGCACTACC TCCGGTCTGT GCACAGCAGC CCCACACTCT 721 CAATGATCTC TGCCGCCAGG GGCCTCAGCC CTGCTGATGT GGCCCACGAA CATCTGAAAG 781 ANAGGGGACT CTTTAGCCTC GCAGCCCCAG GCACCAACCC TTCANANTAT TACCACCAGA 841 TGANCCTCAT GGCAAGCCAC CCCACCCCTT ATGGGGNCCT TCTAATGCNN NNCGGGGGNG 901 CTGCTANCGC ANCCCATCTC CATGACTACN TCAACCCTGN GGATGCNTCA CGATTCTCTA 961 NTCCACGNGT GANCCCNCGN NTGANCCNCA NGCGGGCTCT NNNNNTCNNC CCGCNNNCNN 1021 ANGNCANCNN CNANNNNNNA NCNNNNNNNN NN //
Primer: bGH-M_R (Pr0747) Region: BGH p(A), insert 3' Sequence file: RDB17729_A9Kfb.seq
>08065_17729_A9Kf_3_bGH-M_R_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGGGNNNNN NNNNNACAGN NGGCTGGCAA CTAGAAGGCA 61 CANGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTAGGT 121 CATCATGTTT AAAAACTTGC TCTCTTCAGC CAGGCTGGTG AGCATGGAAC TCATGTCGCC 181 CACGGCCATG TTGCTGATGC CCGCAGGGAT GGAGGGCAGT GTCAAGGAAT TCCGGGGTGT 241 GGTGAGGCGT GAGGAATTCT GGGAGAGATT GTGGAGAAGG GTTGGGCTCA GTGCACCAGA 301 AAACAAACTC GAGTGATCAC CATCATCCAT GATGGCATCA AAGTCAATCT GGGGGGGCTC 361 CAGGAGCTGG GAGTCCACGG TGCTAGACAC CTGGTTGACT CCCGGTGAAG GCAAAGGCTC 421 AGGCTGGATA CTGTCTGAGC AAGCTTGTGG TTGTGGCGGC TGGGGCTGCA CGGCTGGGCA 481 GCCTCCATTC TGTTCATACA TGTGGATCTG GCCATAGTAG TATAGCGTGT TCTGATCTTG 541 TGCCCGCCCT GTGTCCTGAG ATGGCTGAGG AGCTAGGGAT ACCATGGTCC CTGCTGGTCC 601 CTTGGGCATA GCTTCTGCTG TCTCTTGGTT GGCTGATGTG ACCATGGCTT GGCCAGTGGT 661 CCTGGCATAC AACTGCTGTT GCCCAGAACG TACTCCACGG TGACGGCTGG CCGTGTTGGG 721 TTCCAGGGGT GGCCGANGCT GCATGAGACC AAAGCCCGCG TTCTGCTGGG ACTCCATGAA 781 GCCAGGCTGA TGGGATGACA GANNGTTGGA NCCCTGNTGG TANCTGTCCT GCTGGGGATA 841 GCTCNNGANT GGGTGANGTT GTGANNCCGC ANCCNNGGAN GAANTGGGGN NCCCNNGCTC 901 CNGTTGATGN NNNNNCATGA TGGTGGNANT GGGGNNAACT TNNCNTTTGC TGANGGANNC 961 CCNNGNNGNN NGNNTNNNNT GNNNTATTNN TGNNNNNNNN NAAANNNGNN NCNNNNANNC 1021 NCNNNNNNTG NTNNGNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40pro_ori, NeoR Sequence file: RDB17729_A9Kfc.seq
>08065_17729_A9Kf_3_SV40pro_ori_F_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNGNNN CCTCTGNCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 781 NCGGCTGGGT GTGGCGGANC GCTATCANGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 841 ANAGCTTGNC GGCGAATGGG CTGANCGCTT CCTCGTGCTT TACNGNATCG CCGCTCCCGA 901 TTCGCANCGC ATCNCCTTCT ATCGCCTTCT TGANNANTTC TTCTGANCGG GNNTCTGGGG 961 TNCNAAATGA CCNANCNAGC NACNCCNNNN NTGNCNTCNC GANNTTTNNA NTNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919.
user_report	Mikami, Y., GLI-mediated Keratin 17 expression promotes tumor cell growth through the anti-apoptotic function in oral squamous cell carcinomas. J. Cancer Res. Clin. Oncol. 143 (8): 1381-1393 (2017). PMID 28342001.

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl