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pGL4-phCXCL12

Promoter collection, Human CXCL12 promoter

Catalog number RDB07687
Resource name pGL4-phCXCL12
Alternative name U3001-7
Clone info. PCR amplified human CXCL12 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44386550 to 44385073(NC_000010.11); relatively -1445 to +25, where +1 corresponds to 1 nt of NM_199168.4.
Comment PCR cloning, forward primer: gtgggtggatggatggatg; reverse primer: ggctgacggagagtgaaagt. Entire sequence of promoter region has not been confirmed. Known differences (at least) : G to A substitution at 44386558nt. ; G to A substitution at 44386562nt. ; T to C substitution at 44386190nt.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07687.pdf

          Distribution information

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          RDB07687 pGL4-phCXCL12 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phCXCL12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07687).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07687_A5Dnp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F (Pr0081)
          Region: insert 5'
          Sequence file: RDB07687_A5Dnc.seq check
          >07687_07687_A5Dn_pGL4-4174F_A02_02.ab1
              1 NNNNNNNNNN NNNTNNNNNG GCCNNNCNGG CCNGTNNCTG NNCTCGCTAG CCTCGAGGNN
             61 GTGGGTGGAT GGATGGATGG ATGGATGGAT GGATGGATGG AGTGCCGGCC CACAGCCATC
            121 TAACGGCCAA AGTGGTTTTG GAAAAAAAAT GCACAGAAGA CACCTACTCC CACCAGCGGA
            181 GTTCCGGAGC CCTCGCAGCC TCCTGTTGAC CGCTCCCGCC TAATGCAGCC GCTGACCGCC
            241 CACTCCCCGA CGGCCAGGAC TCCCCAGGGA CAGGGACGTG TCCCCAGGGC AGGCCCCTGG
            301 ATGGACGCGG CGACTGACCC CACTTCGCTG GACGCTGTGC TGGGAAGGAC ACAGAGAGGT
            361 GGCTGGGGCA GCCTGCGGTC ACAAAGCGAG GCCCAAAGGG GCGCTCTCCT CACCCCCACG
            421 CCTCCTGGGT GCCGACCTGC ACCCTCCCTT CGCCACCGGA CTGGGGCCAT CTGGGATGTC
            481 TCGGGGGTAT CCGGAGGGCT AAGCACCGCC GAGGGACGGC TCCGTGGGAA GAGTTTTCTG
            541 GACCCAGAAG GCAGACGCCA GTAGTACTGT CCTAGGAGTC GGAGGTCGGG GTGGGGGAGT
            601 TCTCAGCTCT TTGGGTCGCA CGGAGCTTTT CTTGGGTAAG GCAGTAAGTA CTTAGGTTTA
            661 AAGGACTTAC TTACAGCTAC CATTTATTGA GTACTGTCTG CTTGTCAGAC ACGATGCAGA
            721 GAATTTCGCG GCGTGGGGCG GGTCTCATTG AATCTCCCGT CCCACTCCGC GGGGTGGGCC
            781 TGTGNNTAGC TCATTTCACC ATTGAGANGT CGGAAGTACA AANGCTACAT TCGCTTTTAC
            841 TGANANCCGC CGGCGCCTTC TGCTTTGTTT GTACAGGCGA NGAAACTGAN GNTCGGCTGG
            901 NGGCGCCNNN GGCTNNGANN CCGANCNNCG CNNNNNGCNA NNNNNGGNCN NANNNCCGCA
          961 NATCNNNCTN NGCCGGNNNN NNCNCCNCNN NNNNGNNNNN NNGNNNNNNN NNN
          //
          Primer: pGL4-136R (Pr0079)
          Region: insert 3'
          Sequence file: RDB07687_A5Dnd.seq check
          >07687_07687_A5Dn_pGL4-136R_B02_05.ab1
              1 NNNNNNNNNN NNGGNNNNTT NNNNGGNGGC TTTNNNNACA GTACCGGNNT GCCNAGCTTG
             61 GCCGCCGAGG CCAGATCTTG ATGGCTGACG GAGAGTGAAA GTGCGGCGGT GGGAGGCGCG
            121 CGCCGGGCGC TTTAGAGGGG AGAGCCAGGC GGGGCGGGGC GGGGCGCGCG GTGAGGGGCG
            181 GGCCCGGAGC CGAGTGCTGG CCGTGGCCGG GGTCTCTGAC GCGCGACTCC CTCGGCGGCA
            241 CCCCCCACCC ATCCCCTTCG CCCCTCCTCC GCTCCCTCTG TGCTCCCGAG GCGCAGTGCG
            301 CTCCGGCCTT TGACCTTCTC AGGCTCCGCT GGGCCCCGCA CCGCTGGCCC GGTCTGCAGT
            361 GAGGTCCGCA GCCTGAGCGA CGCCGGCTGG AGGGCCGCTT ATTGTCCCTG TGACAGGGCC
            421 CCACTGGAGA GACTGAGGAC CTTAGGCGTA AAGTGGGGCG CGCGTGGAAA GCTGCGGGAC
            481 GGGGCCTTTC GATGGCGGGA ACTGAATGAG AACCAATGAA AAGCAAACAA GTTAGTCCCG
            541 GGCGGAGGGC CTGCTGGCGG TGGCGGCCCA AGGCAGCCGA GGGGGTCCTG CTTTCTGTGC
            601 GTGGGGGCTC AAGCTCGCCA CCTGCCCGGC TTGCGGCGCA GCCGCCGGCA GAGCTCGATC
            661 TGCGGGAATG AGACCCGTCT TTGCAGTCAG CGTGGCTCGG ACTCCAAGCC CACGGCGCCA
            721 CCAGCCGAGC CTCAGTTTCC TCGCCTGTAC AAACAAAGCA GAANGCGCCG GCGGCTCTCA
            781 NTAAAAGCGA ATGTAGCCTT TGTACTTCCG ANCTCTCAAT GGTGAAATGA NCTNATCACA
            841 GGCCCACCCC GCGGANNGGG ACGGGANNNT CAATGNNNNC CGCCCCACGC CGCGAAATTN
            901 TCNGCATCNN NNCTGANNAN CAGANANNAC TNNATAANNG GNANCTNNAA NTANNNCCTT
          961 TNAANNNANN NNNNNNNN
          //
          Primer: SV40pA_R (Pr0882)
          Region: SV40 pA,Luc 3'
          Sequence file: RDB07687_A5Dne.seq check
          >07687_07687_B1FaS_SV40pA_R_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTAAAGC ATTTTTTTCN CTGCATTCTA GTTGTGGTTT
             61 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT
            121 AGAATTATTA CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT
            181 TGCGGGCGTC CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC
            241 CGCGCAGCTT CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG
            301 TCATGGTTTT ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT
            361 CGGGCAGGCC GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA
            421 GTTCGGCTGG GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA
            481 TGAAGAAGTG CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT
            541 TGTCGATGAG AGCGTTTGTA GCCTCGGGGT TGTTAACGTA NCCGCTCATG ATCATGGGGC
            601 CACGGACGCA CAGCTCGNCG CGCTGGTTCA CACCCAGNGT CNTACCGGTG TNNNAGTNCA
            661 NCACCTTAGC CTCGAANAAN GGNANNACNT TGNNNANNGN NNCNNGNNTN GNNNNCCCCT
          721 TNNGGGNNNN NNNNANNNNN NNNNGNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl