RDB07527 - pGL4-phAPOB

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pGL4-phAPOB

Promoter collection, Human APOB promoter

Catalog number	RDB07527
Resource name	pGL4-phAPOB
Alternative name	U2838-3
Clone info.	PCR amplified human APOB promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 21045468 to 21044033(NC_000002.12); relatively -1395 to +41, where +1 corresponds to 1 nt of NM_000384.3.
Comment	PCR cloning, forward primer: GCAGGCGAAGGTCTTTCTGTG; reverse primer: AACCGAGAAGGGCACTCAGC. Entire sequence of promoter region has not been confirmed. Known differences (at least): substitutions A to G at 21,044,338, T to C at 21,044,910, G to A at 21,044,941, G to T at 21,044,975, A to C at 21,045,347 of NC_000002.12.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human apolipoprotein B (including Ag(x) antigen) genomic DNA
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human apolipoprotein B (including Ag(x) antigen)

External Database
Human apolipoprotein B (including Ag(x) antigen)

Reference sequence

Remarks, protocol and/or map (pdf)	RDB07527.pdf

Distribution information

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Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07527	pGL4-phAPOB	DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGL4-phAPOB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07527).

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Promoter Collection (Firefly Luciferase) (English text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07527_A3Jmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07527_A3Jma.seq
>07527_07527_A3Jm_pGL4-4174F_A10_01.ab1 1 GCCCGGGGTC CAGAACATTT CCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC 61 GAGGATGCAG GCGAAGGTCT TTCTGTGAGT CTCCTCTGAG GGAACTGGGT CTTTATACAT 121 AGTTACTGTT TCAGAATTGA TCCTTCTGGA ATCATCAGTC TTCACCAGTA GCTTGTTACA 181 TCTGGGGGTA TCTCATAATT CAAACAAAGC TGACAAGTTG TAACAATGAG CACACACTGA 241 CTTCTGCAAC AGGCGCTGTC CACTTCCCAT CCGCACTCTA CCGGCTTGCT CCTGGCCGCC 301 TCCCACTCGC CTTCCTGGGT GGTCCCCCAG CAGTTATACC TACCTGGTTG TCGCCCCCTC 361 TATCCTACCA CAATTGCTCA CTAGCGGTTT CCTGCGTACA CAGCTTGTCT CCCTAACCAG 421 AGTGGAGGTG CCTTGGGGAC ACAGCCAGGC TCAGACATTC ACTCAGCTCA TCATAGTGCC 481 ATCCCATCAA TAACCCCTTC TGAGTGATCC TGGGTTAGTA AACCGAGTGT CCCTGAAATT 541 CCACTACCGC TGATTCCCTA CAGCTGGGCA GAGGCAGCGA GCGCTGGCTG AAGTTTCCGG 601 TGGGAAATGG GCAGTGCCTA GAAGGGAAGG AAACGATGCA TGAGAAGGTT CCAGATGTCT 661 ATGAGGAACA TGACGTGTCC TGTCCACTAC TCTGCTTTTC CTCGTCCGCC TCCCCACCAC 721 TGGAGGAAAC CTAGAAGCTG GTGCAGGAAA TCCTCCTCTC AACAACCCAA GAACACTTTG 781 CACAAGAGGG GTGCGCCCTC GGAGGTTGCT CTTCCCCAGA GGCCTCTCCT CGCTGGGGTT 841 TCTTGAAGAC AGATACTTGG ACTCCTGCTG GGACCAGGGC AGGCCACCCA TCCTCAGGGG 901 CAGTGACTGG TCACTCACCA GACCTCCCTG CATCCCCCTT CTCTCTCCTC CCCCAGCACG 961 GGCTGAACCC CGCAGCCACA GATTCTGATC AGGATTAGGG TGTGGGTGCA AATCCAAGGT 1021 CCACCCAAAT GGAAAAGAAG TAACCCGATG GGGAACACGG TCTCCACCCA GACAGCGCCT 1081 CAGGGACCTG GGTTCTCCCT CGTGGCTCCT ATTTCAGGTT CCAAGGAGAG CAGAGATTTT 1141 GTCCCAATGG TTGGGTCATC TTGAGAAAGG CCACCCCCCC TGGGTTACAG GGCCAG //
Primer: pGL4-136R Sequence file: RDB07527_A3Jmb.seq
>07527_07527_A3Jm_pGL4-136R_B10_04.ab1 1 GCCTATGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG 61 CCGCCGAGGC CAGATCTTGA TAACCGAGAA GGGCACTCAG CCCCGCAGGT CCCGGTGGGA 121 ATGCGCGGCC GGCGCCCGCA CCCCATTTAT AGGAAGCCCA GGCTGCAAGA GCGCCAGGAT 181 TGCAAAAGGT CCAAAGGGCG CCTCCCGGGC CTGACCTGTT TGCTTTTCTA CACTGGCTTC 241 TCTTTGAGCC TTGAAGAGCC TCGGGGAGGG GGCCCACCTG GGATGCAGCC GCAGCCACCA 301 GGGGCTGGGT CCCAGGTGGG TTCCCTTCCC CAAGCGTCTT CAGTGCTCTG GCGCGGCCCT 361 TCCTGTGTCT CAGTGGGGCC ATGGCCGGCG CCTCAGGGTC TGAGAAGCCT GCCCTGACCA 421 GGGGTGCCTT CTTCAGATGA CCCACCATGG GGACAAAATC TCTGCTTCTC CTGGACTGAA 481 TTGGGAGCCA CGAGGAGAAC CAGTCCTGAG CGCTGTCTTG GTGGAGACGT GTTCCCATCG 541 GTTACTTCTT TTCCATTTTG GTGGACCTTG GATTTGCACC CACACCCTAA TCCTGATCAG 601 AATCTGTGGC TGCGGGGTTC AGCCCGTGCT GGGGGAGGAG AGAGAAGGGG GATGCAGGGA 661 GGTCTGGTGA GTGACCAGTC ACTGCCCCTG AGGATGGGTG GCCTGCCTGG TCCCAGCAGG 721 AGTCCAAGTA TCTGTCTTCA AGAAACCCCA GCGAGGAGAG GCCTCTGGGG AAGAGCAACC 781 TCCGAGGGCG CACCCCTCTT GTGCAAAGTG TTCTTGGGTT GTTGAGAGGA GGATTTCCTG 841 CACCAGCTTC TAGGTTTCCT CCAGTGGTGG GGAGGCGGAC GAGGAAAAGC AGAGTAGTGG 901 ACAGGACACG TCATGTTCCT CATAGACATC TGGAACCTTC TCATGCATCG TTTCCTTCCC 961 TTCTAGGCAC TGCCCATTTC CCACCGGAAA CTTCAGCCAG CGCTCGCTGC CTCTGCCCAG 1021 CTGTAGGGAA TCAGCGGTAG TGGAATTTCA GGACACTCGG GTTTACTAAC CCCAGGATCA 1081 CTCAGAAGGG TATGGATGGA TGCACTATGA TGAGCTGACT GAATGTCTGA GCTTGGCCTG 1141 TGTCCCCAGC ACTCACTCTG GTTAAGGGGA GAAACAA //
Primer: pAxCALNL_F1 Sequence file: RDB07527_A3Jmc.seq
>07527_07527_A3Jm_pAxCALNL_F1_H11_23_ABI24.ab1 1 ATAATATATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC 61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC 121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC 181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA 241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG 301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG 361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC 421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA 481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC 541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC 601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC 661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG 721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG 781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT 841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC 901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG 961 AACATGCCGA AGCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGGTGTC GGGGATGATC 1021 TGGTTTGCCC GAAGATGGGG GTCGCGGGCA TGACTGAATC GGACACAAGC GGGTGCGGTG 1081 CGGTAGGCTA CGCCCTTTGG GGCATCAGTA CCTGCACTTA CCTGATCATG ATCAGGCCGA 1141 ATGGTATGGT CCGATCGAGC TCTCTCGGCA ACACGAAAGA T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.09

GNP_filter3_RDBDEP_html_231027.pl