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pGL4-phPTEN

Promoter collection, Human PTEN promoter

Catalog number RDB07400
Resource name pGL4-phPTEN
Alternative name U2733(2111)-8
Clone info. PCR amplified human PTEN promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 87862065 to 87863506(NC_000010.11); relatively -1560 to -118, where +1 corresponds to 1 nt of NM_000314.7.
Comment PCR cloning, forward primer: cgtgtttggatgtgggtgct; reverse primer: gcctcggaagaccgaggggag. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human phosphatase and tensin homolog genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human phosphatase and tensin homolog

          Reference sequence
            

          Distribution information

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          RDB07400 pGL4-phPTEN DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07400.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07400_A9Gvp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site,insert5'
          Sequence file: RDB07400_A9Gva.seq check
          >07400_07400_A9Gv_1_pGL4ins_F_G10_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNANNNN NNAGNCTGTC CCCNGTGCAA GTGCAGGTGC CAGAACATTT
             61 CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGGATCGTGT TTGGATGTGG
            121 GTGCTTGTGT AACCAGTTCC CCAAGCGCCA GCCCCGACAG CGCTCCTTCG GGAGGCTGGT
            181 CCGAGCCCCT GTTTCCGCCG CGGCGCAGGA AGGGTTGGGG TTCCGCTGCC TGCACCAGGC
            241 AAGAGCACCC CGAGCAAAGG AAGAAGACGA CTTGCCTCCG GAGCTATCAC TGGGGAGTGG
            301 GAATTTGGAA AGTTCCCCAA CTAGGGACAC ACGTGACCTC CTTCGGAAAG TAGTTCCGAC
            361 TGTGGCCCGT GTATCCTTCC ACCTCCTTTT GAACCCTCCT AGGTCTCCTC GCCCCGCCCA
            421 CTCGCTGGGC TGCAGCTTCC TACCGTTCCG TACTTTCCAC TCAACCCGGT AACCCCAAAC
            481 GTGCACGGTC CGGCCGGGGC GCGCGGAGCC TGGCCCCGGG CGATCCATCC TGCCGGGTTT
            541 TCACGGCGGC CAAGGGGGGG CGGGGCTAGG TGGTCTCTGA GAACCGAGCT TGACTCCGAC
            601 GCCGCGAACC GACCTGGAGC CCGAGGGGAA AGATGCTCGA CTCTCTTGGG GGCACCGGAG
            661 CGGGCGCAGG AGAGGCCTGC GGGGTGCGTC CCACTCACAG GGNTCCTCTT TCAGTTCATT
            721 TAGATAGGTG CCCTTTGGGC CCTTGAAATT CAACGGCTAT GTGTTCACGT TCAGCACGCT
            781 CGGCTGANAG CTTTCATTTT TAGGGCAAAC GAGCCGANTT ACCGGGGGAA NCGAGANGTG
            841 GGGGCGCNNC AANGGNNCCG GANGANGNGA TACACGCNGG CGANACAATA NCANGNTNCN
          901 CTTNNNGCTN ANNNNGACAC NNTNNNGNNN CNNNNTNNGG GGGAANGGGG G
          //
          Primer: pGL4-136R
          Region: insert3'
          Sequence file: RDB07400_A9Gvb.seq check
          >07400_07400_A9Gv_1_pGL4-136R_E01_13_ABI24.ab1
              1 NNTGCCNAGC TTGGCCGCCG AGGCCAGATC TTGATGCCTC GGAAGACCGA GGGGAGGCGG
             61 GAGGCGAGCG AGAGGCGGAC GGGACCGCGC CGGGCGAGGG GAGGGCAGGG CAGGGCAGGG
            121 GGCGGTAGGA GGGGGCAGAG CGGTAGCTCT GGGTGCGAGC GCAGAGTCCC CAAGCCGCAG
            181 GCTCTACTGA GCATGCCCAG TGTAGCTGCC TGGGGCTTGC TCGGGCCGGT TCCCAGCCGC
            241 CAGCCTGCAG CTGCACTTGC TGCGGCTTTT GCAGCAACGC GAGGCGAGGA TAACGAGCTA
            301 AGCCTCGGCC TCTGCCCAGA AACCCAGCCG GAGGCAGGGT AGGCTGTTGT GGGGCGGGGG
            361 TGGAGGACTG ATGATGAAAG CTGAGATGGG TGCGTTGAGC AGTGTCACTG ACTCGAGTCT
            421 GAGGTTACCT GTGCACAGGT GAAAAGGACC AGGTGACCAC GCTGCTCAGT GTAGAGGGAA
            481 ATGCAGGGAC GGTCCCTGCA AGGGGAATAC CCTCCCCCCT TGCCTCTACC CCTAGATTTC
            541 CGCGGCGTGG ACTGCATTCG CTCTTTCCTT TTGCACCGCT GTCGGATCAC AATCGTTCGC
            601 AGAGNTTTGA CTGTAACAGC CTTTGCCTAG AGATTCCCCC TTCCCCCAAA TCTGTGTCCT
            661 CATGGTGTCA GTCTTAGCAC AAAGAGCAAC CTGCTATTGT GTCGCCAGCG TGTATCACCT
            721 CATCCGGCTC CCTTGCAGCG CCCCACCTCT CGCTNCCCCG GTAACTCGGC TCGTTNGCCC
          781 TAAAAATGAA AGCTCTCAN
          //
          Primer: pGL4pA_R
          Region: SV40a(A),Luc
          Sequence file: RDB07400_A9Gvc.seq check
          >07400_07400_A9Gv_1_pGL4pA_R_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TNNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
             61 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
            121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
            181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
            241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
            301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
            361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
            421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
            481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
            541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
            601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
            661 CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
            721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
            781 GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC TCGAANAANG
            841 GCACCACCTT GCCTACTGCG CNNGGCTTGT CGTCCCNTTC GGGGGGTGAT CAGAATGGCG
          901 CTNGNTGNTT CTGTCAGGCC GNANCCNNNG NGGANGNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl