Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBRPyCAG-Klf4-IP

Expression clone of mouse Klf4 cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 26, 7772-7782 (2006))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07102_A8Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB07102_A8Eta.seq
>07102_07102_A8Et_3_pAxCA_F1_A01_01_ABI08.ab1     1 CCATTCCGTT CTTACAATGT TTCATGCCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG    61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGAGTCT GACATGGCTG   121 TCAGCGACGC TCTGCTCCCG TCCTTCTCCA CGTTCGCGTC CGGCCCGGCG GGAAGGGAGA   181 AGACACTGCG TCCAGCAGGT GCCCCGACTA ACCGTTGGCG TGAGGAACTC TCTCACATGA   241 AGCGACTTCC CCCACTTCCC GGCCGCCCCT ACGACCTGGC GGCGACGGTG GCCACAGACC   301 TGGAGAGTGG CGGAGCTGGT GCAGCTTGCA GCAGTAACAA CCCGGCCCTC CTAGCCCGGA   361 GGGAGACCGA GGAGTTCAAC GACCTCCTGG ACCTAGACTT TATCCTTTCC AACTCGCTAA   421 CCCACCAGGA ATCGGTGGCC GCCACCGTGA CCACCTCGGC GCCAGCTTCA TCCTCGTCTT   481 CCCCAGCGAG CAGCGGCCCT GCCAGCGCGC CCTCCACCTG CAGCTTCAGC TATCCGATCC   541 GGGCCGGGGG TGACCCGGGC GTGGCTGCCA GCAACACAGG TGGAGGGCTC CTCTACAGCC   601 GAGAATCTGC GCCACCTCCC ACGGCCCCCT TCAACCTGGC GGACATCAAT GACGTGAGCC   661 CCTCGGGCGG CTTCGTGGCT GAGCTCCTGC GGCCGGAGTT GGACCCAGTA TACATTCCGC   721 CACAGCAGCC TCAGCCGCCA GGTGGCGGGC TGATGGGCAA GTTTGTGCTG AAGGCGTCTC   781 TGACCACCCC TGGCAGCGAG TACAGCAGCC CTTCGGTCAT CAGTGTTAGC AAAGGAAGCC   841 CAGACGGCAG CCACCCCGTG GTAGTGGCGC CCTACAGCGG TGGCCCGCCG CGCATGTGCC   901 CCAAGATTAA GCAAGAGGCG GTCCCGTCCT ACACGGTCAG CCGGTCCCTA GAGGCCCATT   961 TGGAGCGCTG GACCCCAGCT CAGCAACGGG CCACCGGCCA ACACACACGA CTTTCCCCCT  1021 GGGGCGGCAG CTCCCCACCA GGGACTACCC CTAACACTGA GTCCGGAGGA ACTGCTGGAC  1081 AGCAGGACTG TCACCCTGCT GCTCTTCCCC CAGGATCATC CCATCCGGCA ACTACCTTCC 1141 TTCTGTCCAG AACTCAGAAA ATGCC //
Primer: CSII-CMV3' Sequence file: RDB07102_A8Etb.seq
>07102_07102_A8Et_3_CSII-CMV3'_A02_01_ABI08.ab1     1 GGGAAAATTC GGGAATTATC AAGCTTATCG AGCGGCCGCT TAAAAGTGCC TCTTCATGTG    61 TAAGGCAAGG TGGTCCGACC TGGAAAAGGC CCTGTCGCAC TTCTGGCACT GAAAGGGCCG   121 GTGCCCTGTG TGTTTGCGGT AGTGCCTGGT CAGTTCATCG GAGCGGGCGA ATTTCCACCC   181 ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCGCCTGTG TGAGTTCGCA GGTGTGCCTT   241 GAGATGAGAA CTCTTGGTAT AGGTTTTGCC ACAGCCTGCA TAGTCACAAG TGTGGGTGGC   301 TGTTCTTTTC CGGGGCCACG ACCTTCTTCC CCTCTTTGGC TTGGGCTCCT CTGGCAGGCA   361 GGAACCCGGT GGCATGAGCT CTTGATAATG GAGAGAGGGG ACTTGTGACT GCATCTGGTC   421 TGGCAGGAAA GGAGGGTAGT TGGGCCCCGG ATGGGGATGG AATCCTGGGG GAAGAGGCAG   481 GCCAGGGTGA CAGTCCCTGC TGTTCAGCAG TTCCTCGGGA CTCAGTGTAG GGGTAGTCCT   541 GGTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGTGTTG GGCCGGTGGC CGTTGCTGAG   601 CTGGGGTCCA GCGCTCAAAT GGGCCTCTAG GGACCGGCTG ACCGTGTAGG ACGGGACCGC   661 CTCTTGCTTA ATCTTGGGGC ACATGCGCGG CGGGCCACCG CTGTAGGGCG CCACTACCAC   721 GGGGTGGCTG CCGTCTGGGC TTCCTTTGCT AACACTGATG ACCGAAAGGG CTGCTGTACT   781 CGCTGCCAGG GGTGGTCAGA GACGCCTTCA GCACAAACTT GCCCATCAGC CCGCCACCTG   841 GCGGCTGAGG CTGCTGTGGC GGAATGTATA CTGGGTCCAA CTCCGGCCGC AGGAGCTCAG   901 CCACGAAGCC GCCCGAGGGG CTCACGTCAT TGATGTCCGC CAGGTTGAAG GGGGCCCGTG   961 GGAGGTGGCG CAGATTCTCG GCTGTAGAGG AGCCTCCACC TGTGTTGCTG GCAGCCACGC  1021 CCGGGTCACC CCCCGGCCCG GAATCGGATA GCTGAGGCTG CAGTGGAGGG CGCGCTGGCC  1081 AGGTCGCTGC TCGCTGGGGA GGACGAAGGA ATGAAGCTTG GCGCCGAAGG GGTGGGTTCA 1141 CGGGGTTGGC GGGGC //
Primer: CMV_en_R Sequence file: RDB07102_A8Etc.seq
>07102_07102_A8Et_3_CMV_en_R_A01_01_ABI08.ab1     1 TGGGTTTAAT GATTACTATT AATAACTAGC TAGTCAATAA TCAATGTCGA CGATGATATC    61 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG   121 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG   181 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG   241 TAATTTTCCT CCCGACTCTT AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA   301 ACTGACCGCA GCTGGCCGTG CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC   361 AGAGGGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT   421 CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC   481 CCAATCATTA CTATGACAAC AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGCCCCT   541 GGCCTCCGCT TACTCTGGAG AAAAAGAAGA GAGGCTTCCA GAGGCAACTT GTCAAAACAG   601 GACTGGCGCC TTGGAGGCGC TGTGGGGCCA CCCAAATTGA TATAATTAAG CCCCAACCGC   661 CTCTTCCCGC CTCATTTCAG CCTCACCACT ATGTGAATAT AGCTGAATAC ACAGTTTATT   721 GAATAAACAT TAATTTCCAG GAAATACAGT CTTTGTTTTT CCAAAGCGGT CATTGAAAAA   781 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT   841 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT   901 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT   961 TTCGCCCCGA AGAACGTTTT CCCAATGATG AGCACTTTTT AAGTTCTGCT ATGTGGCGCG  1021 GGTATTATCC CGTATTTGAC GCCCGGGCAA GAGCACTCGG GTCGCGGCAT AACACTAATT  1081 CTCAGATGAC TTTGGTTGAT TACTCACAGT TCACAGAAAA GCCATCCTTA ACGGATTGGG 1141 CAATGTGAAC GAGCTAA //
Primer: BGH_rev2 Sequence file: RDB07102_A8Etd.seq
>07102_07102_A8Et_3_BGH_rev2_C02_01_ABI08.ab1     1 CATCGGGGTT AAGGAAAGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG    61 CACAGTCGAG GCTGATCAGC GAGCTCTAGA TCATCGATGC ATGGGGTCGT GCGCTCCTTT   121 CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC   181 AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT   241 AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG   301 CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG   361 AGACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC   421 CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA   481 TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG   541 CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT   601 CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG   661 GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG   721 CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT   781 CAAAGGAAAA CCACGTCCCC GTGGTTCGGG GGGCCTAGAC GTTTTTTTTA ACCTCGACTA   841 AACACATGTA AAGCATGTGC ACCGAGGCCC CAGATCAGAT CCCATACAAT GGGGTACCTT   901 CTGGGGCATC CTTCAGCCCC TTGTTGAATA CGCTTGAGGA GAGCCATTTG ACTCTTTCCA   961 CAACTATCCC AACTCACAAC GTGGCACTGG GGGTTGTGCC CGCCCTTTGC AGGTGTATCT  1021 TTATACACGT GGGCTTTTTG GGCCGGCAGA GCAACCTGTC GCCAGGTGGG GGGGTTCGGC  1081 TGCCTGCAAA GGGGTTCGCT ACAGACGTGA TTTGTCTCCA AGGAAAGCCT TCCAAGAAGG 1141 AACTGTGCCC T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl