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pLefty1-luc deltaSacI

Reporter construct of Lefty1 minimal promoter.

Catalog number RDB07097
Resource name pLefty1-luc deltaSacI
Alternative name pDelSacI-luc, pLefty-luc deltaSacI; stock no 2109
Clone info. Reporter construct with luciferase expression under the Lefty1 minimal promoter (-76 to +82 relative to the transcription start site of the Lefty1).
Comment Generated by digestion of pLefty1-luc with SacI, followed by T4 DNA polymerase treatment and self ligation. See Fig.1C of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone pGL3-basic (Plasmid)
Gene/insert name Mouse Lefty1 genomic DNA
Depositor|Developer Niwa, Hitoshi |
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External Database
Mouse Lefty1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mol. Cell. Biol., 26, 7772-7782 (2006))。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB07097 pLefty1-luc deltaSacI Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB07097z.seq

          Original reference

          original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Reguration of genes in ES cells (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

          2022.05.17

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