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pxCAhFLPe

Expression vector of site-specific recombinase, FLP

Catalog number RDB07083
Resource name pxCAhFLPe
Clone info. Expression vector of site-specific recombinase, FLP. The hFLPe recombinase is expressed under the control of a CAG promoter. To increase the FLPe level in mammalian cells, a humanized FLPe (hFLPe) containing a codon suitable for use in mammals was generated.
Vector backbone pBR327 (Plasmid)
Selectable markers Amp^r
Gene/insert name S. cerevisiae FLP recombinase cDNA
Reference of insert sequence AB434280 (DDBJ accession number)
Depositor|Developer Saito, Izumu | Kondo, Saki |
Other clones in our bank
External Database
S. cerevisiae FLP recombinase
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (J. Mol. Biol., 390, 221-230 (2009)) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          MTA, for use for not-for-profit academic purpose [Word]
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          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (J. Mol. Biol., 390, 221-230 (2009)) を引用し、謝辞の表明を必要とする。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

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          RDB07083 pxCAhFLPe DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pxCAhFLPe was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07083).

          Reference section:

          Kondo, S., Takata, Y., Nakano, M., Saito, I., Kanegae, Y., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content Expression regulation (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13038_A4Lg.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB13038_A4Lga.seq check
          >07083_13038_A4Lg_pAxCA_F1_G12_21.ab1
              1 GGTGCCCGGA AAACCCACGC CGCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG
             61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTGATT TATCGATTTG
            121 CAGACCGTGC ATCATGAGCC AGTTTGATAT CCTGTGCAAG ACACCACCTA AGGTGCTGGT
            181 GCGCCAGTTC GTGGAGAGGT TTGAGAGGCC CTCTGGAGAG AAGATTGCCT CCTGTGCAGC
            241 TGAGCTGACC TACCTGTGCT GGATGATCAC CCACAACGGC ACAGCCATCA AGAGGGCCAC
            301 CTTTATGAGC TACAACACCA TCATTAGCAA CTCCCTGAGC TTCGACATTG TGAACAAGTC
            361 CCTCCAGTTT AAATACAAGA CCCAGAAGGC CACAATCCTG GAGGCCTCCC TGAAGAAATT
            421 GATTCCTGCT TGGGAGTTCA CCATCATCCC CTACAATGGC CAGAAGCACC AGTCTGATAT
            481 CACTGATATT GTGAGCAGTC TGCAACTCCA GTTCGAGTCC TCTGAGGAAG CTGACAAGGG
            541 CAACAGCCAC AGCAAGAAGA TGCTGAAGGC CCTGCTCAGT GAGGGAGAAA GCATCTGGGA
            601 GATCACTGAG AAGATCCTGA ACTCCTTTGA GTACACTTCC AGATTCACCA AGACCAAGAC
            661 CTTGTACCAG TTCCTGTTCC TGGCCACCTT CATCAACTGT GGCAGGTTCA GCGACATCAA
            721 GAATGTGGAT CCCAAATCCT TTAAACTGGT CCAGAACAAG TACCTGGGAG TGATCATCCA
            781 GTGCCTGGTG ACAGAGACCA AGACCTCTGT GAGCAGGCAC ATCTACTTCT TCTCTGCCAG
            841 GGGCAGGATT GATCCACTGG TGTACCTGGA TGAGTTCCTG AGGAACTCTG AGCCAGTGCT
            901 GAAGCGGGTG AACAGGACCG GCAACTCTTC CAGCAACAAG CAGGAGTACC AGCTGCTCAA
            961 AGGACAACCT GGTGAGGTCC TACAACAAAG CTTTGAAGAA AAATGCCCCC CTACCCAATC
           1021 TTTGCCATCA AGAATGGGCC CTAAGTCCCA CATTTGGCAG ACACCTGATG ACCTCGTTCT
           1081 GTCCATGAAA GGCCCTGAAC GAGCCTGAAC AATGGATGTG GGCCAACTGG AGCCGGAATA
          1141 GCCGGGGGCC CTCTCTGGCC CGT
          //
          Primer: pAxCA_R1
          Sequence file: RDB13038_A4Lgb.seq check
          >07083_13038_A4Lg_pAxCA_R1_G03_21.ab1
              1 CAAGGGACCC AGCCGCCCTT CGCCACACCA GCCCACCACC TTCTGAATAG GCAGCCCTGC
             61 ACCTTGAGGA GTGAATTGAT TATCGATTCT AGACTAGTTT ATAATTTGTA CCTCTAGAGT
            121 CAGATGCGTC TGTTGATGTA GGAAGACAGG TAGTCCAGCA CCTCCTGGGA AATGATCCCA
            181 TTCCAGGCAG GGTAGCGAAT GCTGCCCTCG GCACTACCCT TCAGCTGCTC AATGTGCTGC
            241 CACTCCTCAA TTGGGTTGGT CTCATCCTTC AATGCAATCA TCTCCTTGGA GATGGGATCA
            301 TATGCATAGT ACCGAGACAC CAGTGCGAAG TAGTGATCAG GGATTGCTGT GATCTGGTGA
            361 GTATAGGTTG TTCTGGCCAC GGCAGAGGCC CGCTTATCGC TCCAGTTGCC CACAACATTG
            421 GTCAGCTCTG TCAGGCCCTT CATGGACAGG AAGGAGGTCA TCAGGTGTCT GCCAATGTGG
            481 GACTTAGGGC CATTCTTGAT GGCAAAGATT GGGTAGGGGG CATTTTTCTT CAAAGCTTTG
            541 TTGTAGGACC TCACCAGGTT GTCCTTGAGC AGCTGGTACT CCTGCTTGTT GCTGGAAGAG
            601 TTGCCGGTCC TGTTCACCCG CTTCAGCACT GGCTCAGAGT TCCTCAGGAA CTCATCCAGG
            661 TACACCCAGT GGATCAATCC TGCCCCTGGC AGAGAAGAAG TAGATGTGCC TGCTCACAGA
            721 GGTCTTGGTC TCTGTCACCA GGCACTGGAT GATCACTCCC AGGTACTTGT TCTGGACCAG
            781 TTTAAAAGGA TTTGGGATCC ACATTCTTGA TGTCGCTGAA CCCTGCCACA GTTGATGAAA
            841 GGTGGCCAGA ACAGGAACTG GTACAAGGTC TTGGGTCCTT GGTGAATCTG GAATGTACTC
          901 AAAGAGTTCA AGATCTTCTT CAGTGATCTC CCAGATGCTT TTCTCCCTCA CTGAACC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kondo, S., Activities of various FLP recombinases expressed by adenovirus vectors in mammalian cells. J. Mol. Biol., 390, 221-230 (2009). PMID 19414019. [link to RRC of NBRP]

          2024.01.19

          GNP_filter3_RDBDEP_html_240118.pl