RDB07039 - nBLUEneo

nBLUEneo

Plasmid clone to construct knock-in vectors

Catalog number	RDB07039
Resource name	nBLUEneo
Clone info.	nBLUEneo is a Gateway(R) entry clone containing beta-galactosidase gene with nuclear localization signal, and neomycine-resistance gene casette driven by pgk promoter. Coding sequenceis flanked by attL1 and attL2 sites, for recombination.
Comment	ES cells can be selected with G418. Expression of beta-galactosidase was confirmed by the depositor. For sequencing, use the following primers as well as M13F and M13R: LacZ(68->49), TTA AGT TGG GTA ACG CCA GG; neoC1, TCG TGC TTT ACG GTA TCG CCG CTC CCG ATT; neoC2, GCC TGC TTG CCG AAT ATC ATG GTG GAA AAT.
Vector backbone	pDONR221 (Plasmid)
Size of vector backbone	2.6 kb
Selectable markers	Kan^r
Depositor\|Developer	Sasai, Yoshiki \| Ikeya, Makoto \|

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Reference sequence

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Int. J. Dev. Biol., 49, 807-823 (2005)).

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Remarks
Please obtain written approval of the depositor.
Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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Terms and conditions set forth by the DEPOSITOR	This material is provided with written consent of Depositor. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Int. J. Dev. Biol., 49, 807-823 (2005)).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	Please obtain written approval of the depositor. Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供条件	利用者は寄託者から事前に提供承諾書を取得すること。　利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Int. J. Dev. Biol., 49, 807-823 (2005))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 寄託者による提供承諾書 [Word]
備考	寄託者の書面による提供承諾が必要です。 Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB07039	nBLUEneo	Under QC test. Please contact us.	DNA solution

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Electronic file

Original reference

original	Ikeya, M., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289.
original	Ikeya, M., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977.

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

Original, user report and related articles

original	Ikeya, M., Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development, 133, 4463-4473 (2006). PMID 17035289.
original	Ikeya, M., Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol., 49, 807-823 (2005). PMID 16172977.

2022.05.17

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