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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsIL8

Expression vector of human IL8.

Catalog number RDB07023
Resource name pCMFlag_hsIL8
Alternative name SET-0201_7
Clone info. Expression vector of human IL8. Expression has not yet been confirmed.
Comment Derived from Sugano DMC04644. PCR cloning, forward primer: atgacttccaagctggccgtgg; reverse primer: ttatgaattctcagccctcttca.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human IL8 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human IL8
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB07023 pCMFlag_hsIL8 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsIL8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07023).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13985_A6Cep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13985_A6Cea.seq check
          >07023_13985_A6Ce_CMV-Forward_D08_11.ab1
              1 CTTTACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGAATTCAC CATGGACTAC AAGGACGATG ACGATAAGGC
            181 GGCCATGACT TCCAAGCTGG CCGTGGCTCT CTTGGCAGCC TTCCTGATTT CTGCAGCTCT
            241 GTGTGAAGGT GCAGTTTTGC CAAGGAGTGC TAAAGAACTT AGATGTCAGT GCATAAAGAC
            301 ATACTCCAAA CCTTTCCACC CCAAATTTAT CAAAGAACTG AGAGTGATTG AGAGTGGACC
            361 ACACTGCGCC AACACAGAAA TTATTGTAAA GCTTTCTGAT GGAAGAGAGC TCTGTCTGGA
            421 CCCCAAGGAA AACTGGGTGC AGAGGGTTGT GGAGAAGTTT TTGAAGAGGG CTGAGAATTC
            481 ATAAGGCCGC TCGAGCATGC ATCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG
            541 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC
            601 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA
            661 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA
            721 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC
            781 TATGGCTTCT GAGGCGGAAG AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT
            841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
            901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
            961 TTTCCCCGTC AAGCTCTAAA TCGGGGGGGC TCCCTTTAGG TTCCGATTTA GTGCTTTACG
           1021 GCACCCTCGA CCCCCCAAAA AACTTGGATT ATGTGATGGA TCACGGTTAG TTGCGCCAAT
          1081 CGACCATGGC ATTAGGAACG ATTTTCGATC GTTTGAACCG AATTTTG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13985_A6Ceb.seq check
          >07023_13985_A6Ce_SV40pro_F_V2_E08_14.ab1
              1 TGGACTTTTT GGAGCTAGGC TTTTGCAAAA GCTCCCGGGA GCTTGGATAT CCATTTTCGG
             61 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC
            121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
            181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
            241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
            301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
            361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
            421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
            481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
            541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
            601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG
            661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG
            721 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GACATAGCGT TGGCTACCCG TGATATTGCT
            781 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTTCTCGTGC TTTACGGTAT CGCCGCTCCC
            841 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT CTCTGAGCGG GACTCTGGGG
            901 TTCGAATGAC GACAGCGACG CCCACTGCAT CACGAGATTT CGATCCACCG CCGCCTTCTA
            961 TGGAAAGTGG CTCGATCGTT CCGAACGCCG CTGATGATCT TCAGCCGGGG AATTCCATGC
          1021 TGAATCATCG CCATCCAACT GGTAATC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl