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hPrP129M in pTA2

Plasmid clone of human prion cDNA

Catalog number RDB06716
Resource name hPrP129M in pTA2
Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met at codon 129. Nucleotide sequence of 75 to 700 of AB300823.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129M cDNA
Reference of insert sequence AB300823 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
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External Database
Human hPrP 129M
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FEBS J., 275, 2965-2976 (2008))。
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          RDB06716 hPrP129M in pTA2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The hPrP129M in pTA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06716).

          Reference section:

          Kikuchi, Y., Kakeya, T., Nakajima, O., Sakai, A., Ikeda, K., Yamaguchi, N., Yamazaki, T., Tanamoto, K., Matsuda, H., Sawada, J., Takatori, K., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06716_A3Jh.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB06716_A3Jha.seq check
          >06716_06716_A3Jh_3_T7_20bp_D02_11.ab1
              1 TACGTGGGAC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCCAATACTC
             61 GGAATTCCAT ATGAGCAAGA AGCGCCCGAA GCCTGGAGGA TGGAACACTG GGGGCAGCCG
            121 ATACCCGGGG CAGGGCAGCC CTGGAGGCAA CCGCTACCCA CCTCAGGGCG GTGGTGGCTG
            181 GGGGCAGCCT CATGGTGGTG GCTGGGGGCA GCCTCATGGT GGTGGCTGGG GGCAGCCCCA
            241 TGGTGGTGGC TGGGGACAGC CTCATGGTGG TGGCTGGGGT CAAGGAGGTG GCACCCACAG
            301 TCAGTGGAAC AAGCCGAGTA AGCCAAAAAC CAACATGAAG CACATGGCTG GTGCTGCAGC
            361 AGCTGGGGCA GTGGTGGGGG GCCTTGGCGG CTACATGCTG GGAAGTGCCA TGAGCAGGCC
            421 CATCATACAT TTCGGCAGTG ACTATGAGGA CCGTTACTAT CGTGAAAACA TGCACCGTTA
            481 CCCCAACCAA GTGTACTACA GGCCCATGGA TGAGTACAGC AACCAGAACA ACTTTGTGCA
            541 CGACTGCGTC AATATCACAA TCAAGCAGCA CACGGTCACC ACAACCACCA AGGGGGAGAA
            601 CTTCACCGAG ACCGACGTTA AGATGATGGA GCGCGTGGTT GAGCAGATGT GTATCACCCA
          661 GTACGAGAGG GAATC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl