Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsNanog

Expression vector of human NANOG.

Catalog number RDB06601
Resource name pCMFlag_hsNanog
Alternative name SET-0224_9-1
Clone info. Expression vector of human NANOG. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgagtgtggatccagcttgt; reverse primer: tcacacgtcttcaggttgcat. Nucleotide substitution c1014t (silent) and t747c (silent) to NM_024865.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Nanog cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human Nanog
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 発表に際し、理研BRCから入手したことを明記すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB06601 pCMFlag_hsNanog DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06601.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12819_A4Gb.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12819_A4Gba.seq check
          >06601_12819_A4Gb_CMV-Fowaed_A01_01.ab1
              1 TGTATTCCAC TCCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
            121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG
            181 ATAAGGCGGC CATGAGTGTG GATCCAGCTT GTCCCCAAAG CTTGCCTTGC TTTGAAGCAT
            241 CCGACTGTAA AGAATCTTCA CCTATGCCTG TGATTTGTGG GCCTGAAGAA AACTATCCAT
            301 CCTTGCAAAT GTCTTCTGCT GAGATGCCTC ACACGGAGAC TGTCTCTCCT CTTCCTTCCT
            361 CCATGGATCT GCTTATTCAG GACAGCCCTG ATTCTTCCAC CAGTCCCAAA GGCAAACAAC
            421 CCACTTCTGC AGAGAAGAGT GTCGCAAAAA AGGAAGACAA GGTCCCGGTC AAGAAACAGA
            481 AGACCAGAAC TGTGTTCTCT TCCACCCAGC TGTGTGTACT CAATGATAGA TTTCAGAGAC
            541 AGAAATACCT CAGCCTCCAG CAGATGCAAG AACTCTCCAA CATCCTGAAC CTCAGCTACA
            601 AACAGGTGAA GACCTGGTTC CAGAACCAGA GAATGAAATC TAAGAGGTGG CAGAAAAACA
            661 ACTGGCCGAA GAATAGCAAT GGTGTGACGC AGAAGGCCTC AGCACCTACC TACCCCAGCC
            721 TCTACTCTTC CTACCACCAG GGATGCCTGG TGAACCCGAC TGGGAACCTT CCAATGTGGA
            781 GCAACCAGAC CTGGAACAAT TCAACCTGGA GCAACCAGAC CCAGAACATC CAGTCCTGGA
            841 GCAACCACTC CTGGAACACT CAGACCTGGT GCACCCAATC CTGGAACAAT CAGGCCTGGA
            901 ACAGTCCCTT CTATAACTGT GGAGAGGAAT CTCTGCAGTC CTGCATGCAG TTCCAGCCAA
            961 ATTCTCCTGC CAGTGACTTG GAGGCTGCTT TGGAAGCTGC TGGGGAAAGG CCTTAATGTA
           1021 ATACAGCAGA CCACTAGGTA TTTTAGTACT CCACAAAACC ATGGATTTTA TTCCTAACTA
           1081 CTCCATGAAC ATGCAACCTG AAGACGTGTG AGCCGCCTCG GAGCAATGCA ATCTAGAGGC
          1141 CCTATTCTTA TAGTGTCACC TAAAATGCTA GAAGCCTCGC TGAATTCAAG ACTCTCTC
          //
          Primer: BGH_rev
          Sequence file: RDB12819_A4Gbb.seq check
          >06601_12819_A4Gb_BGHrev_B01_04.ab1
              1 CTAGCACTCG CAATTTTAGG GTGAACTATA GGAATAGGGC CCTCTAGATG CATGCTCGAG
             61 CGGCCTCACA CGTCTTCAGG TTGCATGTTC ATGGAGTAGT TTAGGAATAA ATCCATGGTT
            121 TGTGGAGTAC TAAAATACCT AGTGGTCTGC TGTATTACAT TAAGGCCTTC CCCAGCAGCT
            181 TCCAAAGCAG CCTCCAAGTC ACTGGCAGGA GAATTTGGCT GGAACTGCAT GCAGGACTGC
            241 AGAGATTCCT CTCCACAGTT ATAGAAGGGA CTGTTCCAGG CCTGATTGTT CCAGGATTGG
            301 GTGCACCAGG TCTGAGTGTT CCAGGAGTGG TTGCTCCAGG ACTGGATGTT CTGGGTCTGG
            361 TTGCTCCAGG TTGAATTGTT CCAGGTCTGG TTGCTCCACA TTGGAAGGTT CCCAGTCGGG
            421 TTCACCAGGC ATCCCTGGTG GTAGGAAGAG TAGAGGCTGG GGTAGGTAGG TGCTGAGGCC
            481 TTCTGCGTCA CACCATTGCT ATTCTTCGGC CAGTTGTTTT TCTGCCACCT CTTAGATTTC
            541 ATTCTCTGGT TCTGGAACCA GGTCTTCACC TGTTTGTAGC TGAGGTTCAG GATGTTGGAG
            601 AGTTCTTGCA TCTGCTGGAG GCTGAGGTAT TTCTGTCTCT GAAATCTATC ATTGAGTACA
            661 CACAGCTGGG TGGAAGAGAA CACAGTTCTG GTCTTCTGTT TCTTGACCGG GACCTTGTCT
            721 TCCTTTTTTG CGACACTCTT CTCTGCAGAA GTGGGTTGTT TGCCTTTGGG ACTGGTGGAA
            781 GAATCAGGGC TGTCCTGAAT AAAGCAGATC CATGGAGGAA GGAAGAGGAG AGACAGTCTC
            841 CGTGTGAGGC ATCTCAGCAG AAGACATTTG CAAGGATGGA TAGTTTTTCT TTCAGGCCCA
            901 CAAATCACAG GCATAGGTGA AGATTCTTTT ACAGTTCGGA TGCTTCAAAG CAAGGGCAAG
            961 CTTTGGGGAC AAGCTGGATC CACACTCATG GGCGCCCTTA TCGTCATCGT TCCTTGTAGT
           1021 TCATGGTGAA ATTCAGCTTG GGGTCTCCCT ATAAGTGGAG TCGGTATTAA TTTCGGAATA
           1081 GCCCAGTTAA GCAGTGGGGG TTCTTCTAGT TAGGCCAGAG GAAGCCTCCT GCTTATTATA
           1141 GGACCTTCCC ACCGGTACAC GGCTTACGGC CATTGCGTCA TGGAGGGCGA GAGTTGTTTA
          1201 CGAACACTTT TTGGGAAGAG TCTCGCTGGA ATGTTTG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl