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pDUAL-GFH31

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Catalog number RDB06544
Resource name pDUAL-GFH31
Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364132 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB06544zzk03.seq provided by the depositor

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
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            RDB06544 pDUAL-GFH31 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pDUAL-GFH31 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06544).

            Reference section:

            Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
            Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14202_A6Fnp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev (Pr0048)
            Region: ADH1 ter,insert 3'(6xHis,FLAG,GFP(S65C)3')
            Sequence file: RDB14202_A6Fne.seq check
            >06544_14202_A6Fn_o_2_ADH_rev_A12_03.ab1
                1 NNNNNNNNNN NNNNNNNANN TTTCGTTTTN NACCTAAGAG TCACTTTAAA ATTTGTATAC
               61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
              121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
              181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
              241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
              301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
              361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
              421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
              481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
              541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
              601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
              661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
              721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
              781 CAAATAAATT TAANGGTAAG TTTTCCGTAT GTTGCATCAC CNTNCACCCT CTCCACNNAC
              841 AGAAAATTTG NGCCNNTNNA CNTCACCATC NNNNTCNNNN AGNNNNGGNN NNNNNNCNNN
            901 NNAAAGNNCN NNNNCNTNNN NNNNNNN
            //
            Primer: LEU1pro_F (Pr0370)
            Region: leu1 pro,Leu1 5'ORF,ura4 ter
            Sequence file: RDB14202_A6Fnf.seq check
            >06544_14202_A6Fn_o_2_LEU1pro_F_B12_06.ab1
                1 NNNNNNNNNA NNNNNTTGAN TTTCCCGAAA CCNGGCATTT TTGCGGTAGG GTCTGAAACT
               61 TTCACGAAAC CATTTCACAA CAAAACTTCT TCAACAACAT GTGTGCAAAG AAAATCGTCG
              121 TCTTACCAGG AGACCATATT GGCCCTGAAA TTGTTGCTTC TGCCTTGGAG GTTTTGAAAG
              181 TCGTTGAGAA GAAGCGACCT GAGTTAAAAC TCGAGTTTGA AGAACACAAG ATTGGAGGTG
              241 CCTCTATTGA TGCCTATGGA ACCCCTTTGA CTGACGAGAC TGTGAAGGCT TGTTTGGAAG
              301 CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG GACCAACCCC AATTGTCGTC
              361 CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT
              421 GCAACTTTGC CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCTGAA ATCGTTGAAG
              481 GTGTCGATTT TTGTGTTGTA CGAGAACTTA CTGGAGGTTG TTACTTTGGT GAGCGCACTG
              541 AGGACAACGG ATCGGGTTAT GCTATGGACA CTTGGCCTTA CAGTTTGGAA GAAGTTTCTC
              601 GTATTGCTCG TTTGGCGGCC GCGGGCCCAA CACCAATGTT TATAACCAAG TTTTATCTTG
              661 TTTGTCTACA TGGTATTTTA CATTCATCTA CATACATCTT TCATTGGCTT TGTACATAGT
              721 TATCATTACA AGTCTAAAAA AATTCACTCT TTTCTTATTC AATGTCAATC CAAGAGAAAA
              781 GANTGTGGTA ATGTTGTANG AGCATGTTTA ATAAATTACT ATAGCAAATT ACTTTTTATT
              841 CCCAAGGTGT TTATCTATAA TAGTTAATAT TTNAGTCGCT ACNTAAAATT TTACCNAAGA
              901 GTANTTGTAT ACTAATTCTA AATGCCTTCN GANATAAAAC GCCNNNNAAA ANNAANNCAA
            961 ACNNNNCATC NNNTTTTTCN NNNNNNNNN
            //
            Primer: URA4pro_F (Pr0371)
            Region: ura4 pro,ura4
            Sequence file: RDB14202_A6Fng.seq check
            >06544_14202_A6Fn_o_2_URA4pro_F_D12_12.ab1
                1 NNNNNNNNNN NNNNTTGTNN NNNNNNGNAC CTCTTTTTTG CTTGGNTCGA AATTAAAGGT
               61 TTAAAAGCAA AGTTATGGAT GCTAGAGTAT TTCAAAGCTA TTCAGCTAGA GCTGAGGGGA
              121 TGAAAAATCC CATTGCCAAG GAATTGTTGG CTTTGATGGA AGAAAAGCAA AGCAACTTGT
              181 CAGTCGCGGT CGATTTGACG AAGAAATCCG AAATCTTAGA ATTGGTAGAT AAAATTGGAC
              241 CCTATGTCTG TGTTATCAAG ACACATATTG ACGTTGTCGA GGATTTCGAC CAGGATATGG
              301 TAGAAAAACT GGTGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG GATCGCAAAT
              361 TCGCAGACAT TGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTGTAC AAAATTGCTT
              421 CTTGGGCTCA TATCACAAAT TGCCATACAG TGCCAGGCGA GGGTATTATA CAAGGCCTCA
              481 AAGAAGTTGG TTTACCTTTG GGACGTGGTC TCTTGCTTTT GGCTGAAATG TCTTCCAAAG
              541 GCTCTTTGGC TACTGGTTCC TACACAGAGA AAACCTTAGA ATGGTTTGAG AAGCATACCG
              601 ATTTTTGCTT TGGCTTTATA GCTGGTCGTC GATTTCCTAA CCTTCAAAGC GACTACATAA
              661 CTATGTCCCC TGGTATCGGC TTGGATGTTA AAGGAGACGG GCTGGGACAG CAATATCGTA
              721 CTCCTGAAGA AGTGATTGTA AACTGCGGTA GCGACATCAT CATTGTTGGT CGTGGAGTCT
              781 ATGGAGCTGG TCGTAATCCT GTTGTCGAAG CCNAGAGATA TAGAGAAGCT GGNTGGAANN
              841 NATATCAGCA AAGANTTTCT CAGCATTAAA AAAAGANTNA TGNAAAATTT TTTTTGGNTG
              901 GTTANTGAAA AAGTCNANGC NNTGNTNGCG TTTGTTTTCC NNNNNGNTTT ATGNCANANN
            961 NNNTTTAGAN NNAGTANNNN NNTANNNTTT NGNNNAANNT TNNNNNNNNN N
            //
            Primer: f1ori_R3 (Pr0142)
            Region: Leu1 3',ars1
            Sequence file: RDB14202_A6Fnh.seq check
            >06544_14202_A6Fn_o_2_f1ori_R3_C12_09.ab1
                1 NNNNNNNNNN NNNNNGCGGN NNNNATNNCG CACAGATGCG TAAGGAGAAA ATACCGCATC
               61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
              121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
              181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
              241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
              301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
              361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
              421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
              481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
              541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
              601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
              661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
              721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGANG
              781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTNGNANTN
              841 CGAAATGTAA AATCGGGGTA TGGNTAGTAN CGGCAGCGTT CATTATGGNA TATNAAAGAN
              901 AAAAAGTNNN GNAAGCGANA NNNAANGGGN NACNNNGNCN TCGTCTATNA NTNNAGCTAA
              961 AAANNNNANT TTNNNTNNNA NTTTTTNNAN NTTNNNTNNN NNNNNNTTNN NNNNAANNNN
            1021 NNNNNN
            //
            Primer: cam1-pro_F (Pr1250)
            Region: cam1 pro,insert 5'(GFP(S65C)5')
            Sequence file: RDB14202_A6Fni.seq check
            >06544_14202_B2IrF_cam1-pro_F_B06_06_ABI24.ab1
                1 NNNNNNNNNA NGNNNNNGAG CAAACNCTAA CCGCCAAAAA TAAAGGTTAA AAAAAGGANT
               61 AGAAGGACTT TAGAGTTACT GATGCTAGCG TCGACAGGCC TGGATCCGGA TATCATGAGT
              121 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT
              181 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT
              241 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CGTGGCCAAC ACTTGTCACT
              301 ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG ATCACATGAA ACAGCATGAC
              361 TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA GAACTATATT TTTCAAAGAT
              421 GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA
              481 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA
              541 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA
              601 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT
              661 CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC
              721 ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG
              781 TTTGTAACAG CTGCTGGGAN TACACATGGC ATGGATGAAC TATACAAACC CATGGACTAC
              841 NANGACGANG ATGACAAACA TCACCACCAT CACCACTGAN NTCTCGACTC TAGANGANCC
              901 GGCGCGCCNC TTCTNAATNA NCGAANTTCT NANGANTNNT GANTTTTNTT ATTNAATAAN
              961 TTATAAAAAA AANTAAGTGN NNANNANTTT TAAAGTGNNN NNNNNNTTTN AAAANNAAAA
            1021 NTNNNNNNNN NN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
            original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

            2024.03.26

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