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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFFH1-ccdB2

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06506
Resource name pFFH1-ccdB2
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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            RDB06506 pFFH1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06506z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12291_A3Jm.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12291_A3Jma.seq check
            >06506_12291_A3Jm_nmt1Fw_E01_13.ab1
                1 ATGGAAAATT TAATTAAATC AAGGAAAAAA CGTAAACTCT CGGCCTACTG GATGGTTCAG
               61 TCACCCAACG ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT
              121 TGTCATTCGG CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA
              181 TTGAAGATAT ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA
              241 TTATTTCAAT CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC
              301 AGGCCTGGAT CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG
              361 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA
              421 AAACACAACA TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA
              481 TGCTTCCGGC TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC
              541 TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG
              601 GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC
              661 CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAA GAAAAATAAG CACAAGTTTT
              721 ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCCGTATG
              781 GCAAATGAAA AGACGGTGAG CTGGTGATAT GGGATAGTGT TCACCCCTTG TTACACCGTT
              841 TTCCATGAGC AAAACTGAAA ACGTTTTCAT CGCTCTGGAG TTGAATACCA CGACGATTTC
              901 CCGGCAGTTT CTACACATAA TATTCGCAAA GATGGTGGCG TGTTACGGTG AAAAACCCTG
              961 GGCCCTAATT TCCCTAAAGG GGTTTAATTT GAGAATATGT TTTTTCGGTC TCAGCCAAAT
             1021 CCCCTGGGGT GAGTTTCACC CAGTTTTTGA TTTAAAACGT GGCCCAATAA TGGGAACAAA
             1081 TTTTCTTCCG CCCCCGTTTT CACCATGGGG CCAAAATAAA TTATTAACGC AAAGGCCGAA
             1141 CAAAGGGTGG CTGGAATGGC CCGCGTGGCG CGAATCCAGG TCAATTCATG GCCGGTTCTG
            1201 TGTGAATGGG CTTCCCAAGT GTTCGGGGGC CGAGGAAAAT TGCCTCTTAT ATGATG
            //
            Primer: nmt1Rv
            Sequence file: RDB12291_A3Jmb.seq check
            >06506_12291_A3Jm_nmt1Rv_F01_16.ab1
                1 AGCGCCCCGG GTCTGAGATC TCAGTGGTGA TGGTGGTGAT GTTTGTCATC GTCGTCCTTG
               61 TAGTCCATGG GTTTATCGTC ATCATCCTTA TAATCCATGA TATCAACCAC TTTGTACAAG
              121 AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
              181 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GTCGACCTGC
              241 AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA GGTTAATGGC
              301 GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA TAACGGAGAC
              361 CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA TATGCACCAC
              421 CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA GGGGGATCAC
              481 CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA GACGATAACG
              541 GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG TTCTCGTCAG
              601 CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC TGATTTTCCG
              661 CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG CTTACCAGAC
              721 CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT CAACTGTCAC
              781 TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA CATATCAGTA
              841 TATATTCTTA TACCGCAAAA ATCAGCGCGC AAAATACGCA TACTGTTATC TGGCTTTTAG
              901 TAAGCCGGAT CCAGATCTTT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC
              961 ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAAACC TGAATCGCCA
             1021 GCGGCATCAG CACCTTGTCG CCCTTGCGTA TAATATTTTG CCCATGGTGA AACGGGGGCG
             1081 GAGAGTGTCC ATATTGCCAC GTTTAATTCA ACTTGTGACT TCACCAAGGA ATTGCTTGAA
            1141 GAACGAAAAA CATATATTTC CTTCCGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl