Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEM3Zf_hsLIN28

Plasmid clone of human LIN28 cDNA.

Catalog number RDB06427
Resource name pGEM3Zf_hsLIN28
Alternative name SET-0225_8
Clone info. Plasmid clone of human LIN28 cDNA.
Comment PCR cloning, forward primer: ggctccgtgtccaaccagcagtt; reverse primer: tcaattctgtgcctccgggagca.
Vector backbone pGEM-3Zf(+) (Plasmid)
Size of vector backbone 3,197 bp
Selectable markers Amp^r
Gene/insert name Human LIN28 cDNA
Depositor|Developer Nakamura, Yukio |
Other clones in our bank
External Database
Human LIN28
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB06427 pGEM3Zf_hsLIN28 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13526_A5Ipp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13526_A5Ipa.seq check
          >06427_13526_A5Ip_M13(-40)_C06_09.ab1
              1 AAGGCGGGAA TGTATACGAC TCACTATAGG GCGAATTCGA GCTCGGTACC CGGGGATCCT
             61 CTAGAGTCGG CTCCGTGTCC AACCAGCAGT TTGCAGGTGG CTGCGCCAAG GCGGCAGAAG
            121 AGGCGCCCGA GGAGGCGCCG GAGGACGCGG CCCGGGCGGC GGACGAGCCT CAGCTGCTGC
            181 ACGGTGCGGG CATCTGTAAG TGGTTCAACG TGCGCATGGG GTTCGGCTTC CTGTCCATGA
            241 CCGCCCGCGC CGGGGTCGCG CTCGACCCCC CAGTGGATGT CTTTGTGCAC CAGAGTAAGC
            301 TGCACATGGA AGGGTTCCGG AGCTTGAAGG AGGGTGAGGC AGTGGAGTTC ACCTTTAAGA
            361 AGTCAGCCAA GGGTCTGGAA TCCATCCGTG TCACCGGACC TGGTGGAGTA TTCTGTATTG
            421 GGAGTGAGAG GCGGCCAAAA GGAAAGAGCA TGCAGAAGCG CAGATCAAAA GGAGACAGGT
            481 GCTACAACTG TGGAGGTCTA GATCATCATG CCAAGGAATG CAAGCTGCCA CCCCAGCCCA
            541 AGAAGTGCCA CTTCTGCCAG AGCATCAGCC ATATGGTAGC CTCATGTCCG CTGAAGGCCC
            601 AGCAGGGCCC TAGTGCACAG GGAAAGCCAA CCTACTTTCG AGAGGAAGAA GAAGAAATCC
            661 ACAGCCCTAC CCTGCTCCCG GAGGCACAGA ATTGAGACCT GCAGGCATGC AAAGCTTGAG
            721 TATTCTATAG TGTCACCTAA AATAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT
            781 GAAATTGTTA TCCGCTCACA ATTCCACACA AACATACGAG CCGGGAAGCA TAAAGTGTAA
            841 AGCCTGGGGT GCCTAAATGA GTGAGCTAAC TCACATTAAA TTGCGTTGCG CTCACTGCCC
            901 GCTTTCCAGT CGGGAAAACC CTGTTCGTGC CAGCTGCATT AATGAATCGG GCCAAACGCG
          961 CGGGAGGAGA AGCGGGTTTT GCGTATTTGG GCGGCTCT
          //
          Primer: Reverse2
          Sequence file: RDB13526_A5Ipb.seq check
          >06427_13526_A5Ip_Reverse2_D06_12.ab1
              1 CAGGAAGGAA TTACGCCAGC TATTTAGGTG ACACTATAGA ATACTCAAGC TTGCATGCCT
             61 GCAGGTCTCA ATTCTGTGCC TCCGGGAGCA GGGTAGGGCT GTGGATTTCT TCTTCTTCCT
            121 CTCGAAAGTA GGTTGGCTTT CCCTGTGCAC TAGGGCCCTG CTGGGCCTTC AGCGGACATG
            181 AGGCTACCAT ATGGCTGATG CTCTGGCAGA AGTGGCACTT CTTGGGCTGG GGTGGCAGCT
            241 TGCATTCCTT GGCATGATGA TCTAGACCTC CACAGTTGTA GCACCTGTCT CCTTTTGATC
            301 TGCGCTTCTG CATGCTCTTT CCTTTTGGCC GCCTCTCACT CCCAATACAG AATACTCCAC
            361 CAGGTCCGGT GACACGGATG GATTCCAGAC CCTTGGCTGA CTTCTTAAAG GTGAACTCCA
            421 CTGCCTCACC CTCCTTCAAG CTCCGGAACC CTTCCATGTG CAGCTTACTC TGGTGCACAA
            481 AGACATCCAC TGGGGGGTCG AGCGCGACCC CGGCGCGGGC GGTCATGGAC AGGAAGCCGA
            541 ACCCCATGCG CACGTTGAAC CACTTACAGA TGCCCGCACC GTGCAGCAGC TGAGGCTCGT
            601 CCGCCGCCCG GGCCGCGTCC TCCGGCGCCT CCTCGGGCGC CTCTTCTGCC GCCTTGGCGC
            661 AGCCACCTGC AAACTGCTGG TTGGACACGG AGCCGACTCT AGAGGATCCC CGGGTACCGA
            721 GCTCGAATTC GCCCTATAGT GAGTCGTATT ACAATTCACT GGCCGTCGTT TTACAACGTC
            781 GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG
            841 CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC
            901 TGAATGGCGA ATGGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC
            961 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC
           1021 TTTCCTTTCT CGCACGTTCG CCGGGCTTTC CCCGTCAAGC TCTAATCGGG GGCTCCCTTT
           1081 AGGTTCCGAT TAGTGCTTAC GGCACTCGAC CCGAAAAACT GGATTAGGTG AATGATTCAC
          1141 GTTAGTGGGT CAATCGCCCC TGGAAATAGA AACCGGATTC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl