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CSII-EF-mRFP1

Lentivirus vector plasmid of mRFP1 driven by EF-1 alpha promoter.

Catalog number RDB06387
Resource name CSII-EF-mRFP1
Clone info. Lentivirus vector plasmid of mRFP1 driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database
Discosoma sp. RFP

            Reference sequence
              
             
            Sequence RDB06387z.seq
            Remarks, protocol and/or map (pdf) RDB06387.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering Please visit Ordering instruction.[link] 
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06387 CSII-EF-mRFP1 DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-EF-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06387).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content


            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB07977_A3G2p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxEF_F1
            Sequence file: RDB07977_A3G2a.seq check
            >06387_07977_A3G2_pAxEF_F1_E05_14.ab1
                1 ATGTTTTCTC ATTTCAGTGT CGTGACACGC TACCGGTCTC GACTGGATCC GATGGCCTCC
               61 TCCGAGGACG TCATCAAGGA GTTCATGCGC TTCAAGGTGC GCATGGAGGG CTCCGTGAAC
              121 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
              181 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT
              241 CAGTTCCAGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG
              301 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC
              361 GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG
              421 CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG
              481 GAGGCCTCCA CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGATG
              541 AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCCGAGG TCAAGACCAC CTACATGGCC
              601 AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AAGACCGACA TCAAGCTGGA CATCACCTCC
              661 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCG CCGAGGGCCG CCACTCCACC
              721 GGCGCCTAAG AATTCGCGGC CGCGGATCCT CTAGAGTTAA CATCGAGGGA TCAAGCTTAT
              781 CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT
              841 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC
              901 CCGTATGGCT TTCATTTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG
              961 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC
             1021 CCCCACTGGG TTTGGGGCAT TTGCCACCAC CTGTCAGCTC GTTTCGGGGA CTTTCGCTTT
            1081 TCCCCATCCT TATGGCACGG GCGAACCTCA TCGCCCGTCC TACCTTTGAT G
            //
            Primer: CMV-Forward
            Sequence file: RDB07977_A3G2b.seq check
            >06387_07977_A3G2_CMV-Forward_D05_11.ab1
                1 AGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
              121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
              181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
              241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
              301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
              361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
              421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
              481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
              541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
              601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
              661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AAGGAAGCTT
              721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA
              781 TCTTCAGACC CTGGAGGAGA GATATGAGGG ACATTGGAGA GTGATTATAT AAATAATAAA
              841 GTAGTAAAAA TTGACCATTT AGAGTAGCAA CCCAACAGCA AAGAGAAGGA GTGGTGCAGA
              901 GAGAAAAAGA GCAGTGGGGA ATAGGAGCTT TGGTCTGGAT CTGGGAGCAG CAGAGCACTT
              961 ATGGGCGCAA CGTTCATTGA CGCTGACCGG TTACAGGCCG ACACATATGG CCTGCATATG
            1021 TGGCACAGTC GGAACCA
            //
            Primer: BGH_rev
            Sequence file: RDB07977_A3G2c.seq check
            >06387_07977_A3G2_BGH_rev_C05_08.ab1
                1 GCATCGGGGT CTGCTAGAGA TTTTCCACAC TGACTAAAAG GGTCTGAGGG ATCTCTAGTT
               61 ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC ACTCAAGGCA AGCTTTATTG
              121 AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC CAGGCTCAGA TCTGGTCTAA
              181 CCAGAGAGAC CCAGTACAGT CCGGATGCAG CTCTCGGGCC ATGTGATGAA ATGCTAGGCG
              241 GCTGTCAAAC CTCCACTCTA ATACTTCTCT CTCCGGGTCA TCCATCCCAT GCAGGCTCAC
              301 AGGGTGTAAC AAGCGGGTGT TCTCTCCTTC ATTGGCTTCT TCTACCTTCT CTTGCTCAAC
              361 TGGTACTAGC TTGTAGCACC ATCCAAAGGT CAGTGGATAT CTGATCCCTG GCCCTGGTGT
              421 GTAGTTCTGC CAATCAGGGA AGTAGCCTTG TGTGTGGTAG ATCCACAGAT CAAGGATATC
              481 TTGTCTTCGT TGGGAGTGAA TTAGCCCTTC CAGTCCCCCC TTTTCTTTTA AAAAGTGGCT
              541 AAGATCTACA GCTGCCTTGT AAGTCATTGG TCTTAAAGGT ACCTGAGGTG TGACTGGAAA
              601 ACCCACCTCC TCCTCCTCTT GTGCTTCTAG CCAGGCACAA TCAGCATTGG TAGCTGCTGT
              661 ATTGCTACTT GTGATTGCTC CATGTTTTTC TAGGTCTCGA CGGTATCGAT GCGGGGAGGC
              721 GGCCCAAAGG GAGATCCGAC TCGTCTGAGG GCGAACGCGA AGACGCGGAA GAGGCCGCAG
              781 AGTCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC
              841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CACACAGGCG AGCAGCCATG GAAGACGTCA
              901 GCTTTCCCCG ACACACACGG ATGTCAGTGC CCACAGCGAG CCCTGTCAGC AGCGGCAGCA
              961 GCGGCGATGA GTCCGGCTGG CCATAGGAGG GGAGCAAAGT CCGGAGGACT GAACGGTGAT
            1021 GCCAATGCCT CACATGGATG CTCGCATCAG TGCACTCAGG CCA
            //
            Primer: SV40pro_F
            Sequence file: RDB07977_A3G2d.seq check
            >06387_07977_A3G2_SV40pro_F_F05_17.ab1
                1 CCCTGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
               61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC
              121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA
              181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG
              241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG
              301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT
              361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC
              421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG
              481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA
              541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT
              601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA
              661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
              721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
              781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA
              841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT
              901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT
              961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CCTGTCGTGC CAGCTGCATT
             1021 AAATGAAATC GGGCCACGCG CGGGGGAGAG GCGGGTTTGC CGTATGGGCG CTCTTCGCTT
             1081 CTCGCTCACT GAACTCGCTT GCGCCTCGGA TTCGGATTCG GGCCGTTGCC GGCCGGAAAT
            1141 G
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Badiee, P., Intratumoral Anti-PD-1 Nanoformulation Improves Its Biodistribution. ACS Appl. Mater. Interfaces 14 (14): 15881-15893 (2022). PMID 35357803.
            user_report Yoshida, S., Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells. PLoS One, 6 (12): e28856 (2012). PMID 22174914.

            2023.05.22

            GNP_filter3_RDBDEP_html_230512.pl