Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-CMV-mRFP1

Lentivirus vector plasmid of mRFP1 driven by CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17472_A9Hlp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCharry-check_R (Pr0521) Region: mRFP1 5',CMV pro.2 Sequence file: RDB17472_A9Hla.seq
>06386_17572_A9Hl_2_mCherry-check_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCNNCCN NCCTCGNANT TCATCACGCG CTCCCACTTG    61 AAGCCCTCGG GGAANGNCAG CTTCAAGTAG TCGGGGATGT CGGCGGGGTG CTTCACGTAG   121 GCCTTGGAGC CGTACTGGAA CTGAGGGGAC AGGATGTCCC AGGCGAAGGG CAGGGGGCCG   181 CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GTGCCCTCGT AGGGGCGGCC CTCGCCCTCG   241 CCCTCGATCT CGAACTCGTG GCCGTTCACG GAGCCCTCCA TGCGCACCTT GAAGCGCATG   301 AACTCCTTGA TGACGTCCTC GGAGGAGGCC ATCGGATCCA GTCGAGACCG GTAGCGCTAG   361 CGGATCTGAC GGTTCACTAA ACCAGCTCTG CTTATATAGA CCTCCCACCG TACACGCCTA   421 CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT TTGGAAAGTC CCGTTGATTT   481 TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA GACTTGGAAA TCCCCGTGAG   541 TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCNAAAACC GCATCACCAT GGTAATAGCG   601 ATGANTAATA CGTAGANGTA CTGCCAAGTA NGAAAGTCCC ATAANGTCAT GTACTGGGNA   661 TAATGNCAGG CNGGNCCANT TTACCGTCAN TGNNNTCNAT NNGGGGGCGN ANNTNGGCNN 721 ATGNNNNNCN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,mRFP1 3', Sequence file: RDB17472_A9Hlb.seq
>06386_17472_A9Hl_2_PRE_rev_G01_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNCA NCATAGTTAA GANNACCAGT CAATCTTTCA    61 CAAATTTTGT AATCCAGAGG TTGATTATCG ATAAGCTTGA TCCCTCGATG TTAACTCTAG   121 AGGATCCGCG GCCGCGAATT CTTAGGCGCC GGTGGAGTGG CGGCCCTCGG CGCGCTCGTA   181 CTGTTCCACG ATGGTGTAGT CCTCGTTGTG GGAGGTGATG TCCAGCTTGA TGTCGGTCTT   241 GTAGGCGCCG GGCAGCTGCA CGGGCTTCTT GGCCATGTAG GTGGTCTTGA CCTCGGCGTC   301 GTAGTGGCCG CCGTCCTTCA GCTTCAGCCT CATCTTGATC TCGCCCTTCA GGGCGCCGTC   361 CTCGGGGTAC ATCCGCTCGG TGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG   421 GCCGTCGGAG GGGAAGTTGG TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG   481 CAGGGAGGAG TCCTGGGTCA CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC   541 CCACTTGAAG CCCTCGGGGA AGGACAGCTT CNAGTAGTCG GGGATGTCGG CGGGGTGCTT   601 CACGTANGCC TTGGAGCCGT ACTGGAACTG AGGGGACAGG ATGTCNNNNN CGAAGGGCAN   661 GGGGCCGCCC TTGGTCNCCT TCAGCTTGGC GGTCTGGGTG CCCTCGTANG GGGCGGNCCT   721 CGCCCTCGCC CTCNATCTCG AACTCGTGGC CGTTNNNNGN AGNCCTCCAT GCGCACCTTG   781 NANCGCATGN ANCTCCTTNN NTGNNNNNCC TCNNANNNNN GNCNNNNNNA NNNNNNTCNA   841 GACCGGTNNN NGCNNANCNG NNCNNNNNGN NNNNNNAANN NNNNNNCNGC TNATATANNA 901 NNTCCCANNG TANANNNNNN NNNCGNNNNN NTTNNNNTNN NNNNNNNN //
Primer: env_F (Pr0258) Region: cPPT_CTS,CMV en.2,CMV pro.2 Sequence file: RDB17472_A9Hlc.seq
>06386_17472_A9Hl_2_env_F_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNTTCGNN NANTNNNNGN NCTACANATG GCAGTATTCA TCCACAATTT    61 TAAAAGAAAA GGGGGGNNTG GGGGGTACAG TGCAGGGGAA AGAATAGTAG ACATAATAGC   121 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT   181 TTATTACAGG GACAGCAGAA ATTCACTTTG AATTAATTCG ATTAATAGTA ATCAATTACG   241 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC   301 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC   361 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT   421 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT   481 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT   541 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC   601 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGNNTTC CAAGTCTCCA CCCCATTGAC   661 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC   721 TCCGCCCCAT TGACGCAAAT GGGCGGTANG CGTGTACGGT GNGAGGTCTA TATAANCAGA   781 NCTGGTTTAN TGAACCGTCA NANCCGCTAG CGCNACCGGT CTCGANNNGA TCCNANGGCC   841 TCCTCCGANG ANGTCATCNN GGANTTCATG CNCTNNNNGN GCGCNNNGGA NGGNNNCNNG 901 AANNGNNNNN ANNNCNNNNN CNNNNNNNNG NNNANNNNNN NNNN //
Primer: HIV-B_R (Pr0673) Region: HIV-1 psi,5' LTR,CMV pro.1,CMV en.1 Sequence file: RDB17472_A9Hld.seq
>06386_17472_A9Hl_HIV-B_R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NAAANTTTTG NNGTANTCNC CNGTCGCCGC CCCTCGCCTC    61 TTGCCGTGCG CGCTTCAGCA AGCCGAGTCC TGCGTCGAGA GAGCTCCTCT GGTTTCCCTT   121 TCGCTTTCAA GTCCCTGTTC GGGCGCCACT GCTAGAGATT TTCCACACTG ACTAAAAGGG   181 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC   241 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA   301 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGGCA AAACGCGCTG CTTATATAGA   361 CCTCCCACCG TACACGCCTA CCGCCCATTT GCGTCAATGG GGCGGAGTTG TTACGACATT   421 TTGGAAAGTC CCGTTGATTT TGGTGCCAAA ACAAACTCCC ATTGACGTCA ATGGGGTGGA   481 GACTTGGAAA TCCCCGTGAG TCAAACCGCT ATCCACGCCC ATTGATGTAC TGCCAAAACC   541 GCATCACCAT GGTAATAGCG ATGACTAATA CGTAGATGTA CTGCCAAGTA GGAAAGTCCC   601 ATAAGGTCAT GTACTGGGCA TAATGCCAGG CGGGCCATTT ACCGTCATTG ACGTCAATAG   661 GGGGCGTACT TGGCATATGA TACACTTGAT GTACTGCCAA GTGGGCAGTT TANCGTAAAT   721 ACTCCACCCA TTGACGTCAA TGGAAAGTCC CTATTGGCGT TACTATGGGA ACATACGTCA   781 TTATTGACGT CAATGGGCGG GGGTCGTTGG GCGGTCAGCC AGGCGGGNCA TTTACCGTAA   841 GTTATGTAAC GCGGAANTCC NTATATGGNC TATGAACTAA TGANCNCGNN ATTGANTACT   901 ATTAATNNCT AGTCAATAAT CAATGTCANC GCGNATATCT NGNCCGNACA TCGCGAANCA   961 NCGCAAAANG CCNAANCCTA ANNNNANNNN NCATGCNNNN GNNGGTCNNN NNNNGNCNNN 1021 NNNGNANCNN NANNTTNNNN NNNNNN //
Primer: RRE-M_R (Pr0651) Region: RRE,HIV-1 psi Sequence file: RDB17472_A9Hle.seq
>06386_17472_A9Hl_RRE-M_R_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN GCTGTTGCGC CTCNATAGCC CTCAGCAAAT TGTTCTGCTG    61 CTGCACTATA CCAGACAATA ATTGTCTGGC CTGTACCGTC AGCGTCATTG ACGCTGCGCC   121 CATAGTGCTT CCTGCTGCTC CCAAGAACCC AAGGAACAAA GCTCCTATTC CCACTGCTCT   181 TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT GCTACTCCTA ATGGTTCAAT   241 TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG TCCCTCATAT CTCCTCCTCC   301 AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG TCTTACTTTT GTTTTGCTCT   361 TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT CTATCCTTTG ATGCACACAA   421 TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT CTGATCCTGT CTGAAGGGAT   481 GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG TTTCTAACAG GCCAGGATTA   541 ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT GTTTTAATTT ATATTTTTTC   601 TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC TAATTCTCCC CCGCTTAATA   661 CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC TAGTCAAAAT TTTTGGCGTA   721 CTCACCAGTC GCCGCCNCTC GCCTCTTGCC GTGCGCGCTT CAGCAAGCCG AGTCCTGCGT   781 CGAGAGAGCT CCTCTGGTTT CNNTTTCGCT TTCAAGTCCC TGTTCGGGCG CCACTGCTAG   841 AGANTTTNCA CACTGACTAA AANGGTCTGA NGGANCTCTA GTTANCAGAN TCACACAACA   901 GANGGGCNCN CACTACTTGA ANCNNTCAAN GNAAGCTTTA NTGAGGNTTA ANCAGNGGGT   961 TCCNNANTNA GCCNNANANN NCCNANGNNC NNNNNNGGNC TNNNNNGANA NNNNNNNTNN 1021 NNGNNAANNN NNNTNNNNNN ATNNNNNNNC NNNNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGH pA,3' LTR Sequence file: RDB17472_A9Hlf.seq
>06386_17472_A9Hl_2_BGH_rev2_G02_20_ABI24.ab1     1 NNNNNNNNNN NNNNGGGGGN NGGNNNNNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT   121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT   181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG   241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG   301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC   361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT   421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG   481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC   541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT   601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC   661 TGANGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA   721 GCATTGGTAG CTGCTGTATT GCTACTTGTG NNTGCTCCAT GTTTTTCTAG GTCTCGACGG   781 TATCGATGCG GGGNNGCGGC CCAAANGGAN ATCCGANTCG TCTGANGGCG AANGCGAANA   841 NGCGGAANAN GNNGCANNNC NNNCAGCNNN NCNCGGGAAN GNANNNNCCN CNNGANNNAN 901 GNNNNNANNN NNNNANCANA NNNNNNTCCC NNNNNNNNNN //
Primer: Zeo-M_R (Pr0675) Region: Zeo,SV40 pro_ori Sequence file: RDB17472_A9Hlg.seq
>06386_17472_A9Hl_2_Zeo-M_R_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC NNNNCCNCNC CCNGGCNNGG GNGTTGTCCG GCACCNCCTG    61 GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG CGAAGTCGTC   121 CTCCACGAAG TCCCGGGNGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG CTCCGGCGAC   181 GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTTT AGTTCCTCAC   241 CTTGTCGTAT TATACTATGC CGATATACTA TGCCGATGAT TAATTGTCAA CACGTGCTGA   301 TCAGATCCGA AAATGGATAT ACAAGCTCCC GGGAGCTTTT TGCAAAAGCC TAGGCCTCCA   361 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCAG AGGCGGCCTC GGCCTCTGCA   421 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG   481 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT   541 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG   601 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC   661 TGACACACAT TCCACAGAAT TAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA   721 ACCAATANGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATANGGT   781 TGANTGTTGT TCCAGTTTGG AACAAGANTC CACTATTAAA GAACGTGNAN TCCAACGTCN   841 AANNGNCGAA AAANCGTCTA TCNNGNCGAN GGNCCNCTAC NNNNANCATC ACCNNNNNNN 901 NNTTTTTTNG GGNNCNANNN GNNNNNNNNA CTNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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