RDB06321 - pET_HisFLAG-hsATF-2

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pET_HisFLAG-hsATF-2

Expression vector of human ATF-2 tagged with His- and FLAG- epitope

Catalog number	RDB06321
Resource name	pET_HisFLAG-hsATF-2
Clone info.	Expression vector of human ATF-2 tagged with His- and FLAG- epitope.
Comment	Nucleotide substitution T1072G (Phe358Val) to NM_001880.
Vector backbone	pET15b (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r
Gene/insert name	Human CREB2; ATF2 cDNA
Depositor\|Developer	Yokoyama, Kazunari \|

Other clones in our bank

human CREB2; ATF2

External Database
Human CREB2; ATF2

Reference sequence

Distribution information

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RDB06321	pET_HisFLAG-hsATF-2	DNA solution

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How to cite this biological resource

Materials & Methods section:

The pET_HisFLAG-hsATF-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06321).

Reference section:

Murata T., Shinozuka Y., Obata Y., Yokoyama K.K., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971.

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13434_A5Fb.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp Sequence file: RDB13434_A5Fba.seq
>06321_13434_A5Fb_T7_20bp_G08_20.ab1 1 TGTCTCCGTC TAGATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GGCAGCAGCC 61 ATCATCATCA TCATCACAGC AGCGGCCTGG TGCCGCGCGG CAGCCATATG GACTACAAGG 121 ACGATGACGA TAAGGCGGCC ATGAAATTCA AGTTACATGT GAATTCTGCC AGGCAATACA 181 AGGACCTGTG GAATATGAGT GATGACAAAC CCTTTCTATG TACTGCGCCT GGATGTGGCC 241 AGCGTTTTAC CAACGAGGAT CATTTGGCTG TCCATAAACA TAAACATGAG ATGACACTGA 301 AATTTGGTCC AGCACGTAAT GACAGTGTCA TTGTGGCTGA TCAGACCCCA ACACCAACAA 361 GATTCTTGAA AAACTGTGAA GAAGTGGGTT TGTTTAATGA GTTGGCGAGT CCATTTGAGA 421 ATGAATTCAA GAAAGCTTCA GAAGATGACA TTAAAAAAAT GCCTCTAGAT TTATCCCCTC 481 TTGCAACACC TATCATAAGA AGCAAAATTG AGGAGCCTTC TGTTGTAGAA ACAACTCACC 541 AGGATAGTCC TTTACCTCAC CCAGAGTCTA CTACCAGTGA TGAGAAGGAA GTACCATTGG 601 CACAAACTGC ACAGCCCACA TCAGCTATTG TTCGTCCAGC ATCATTACAG GTTCCCAATG 661 TGCTGCTTAC AAGTTCTGAC TCAAGTGTAA TTATTCAGCA GGCAGTACCT TCACCAACCT 721 CAAGTACTGT AATCACCCAG GCACCATCCT CTAACAGGCC AATTGTCCCT GTACCAGGCC 781 CATTTCCTCT TCTGTTACAT CTTCCTAATG GACAAACCAT GCCTGTTGCT ATTCCTGCAT 841 CAATTACAAG TTCTAATGTG CATGTTCCAG CTGCAGTCCC ACTCGTTCGA CCAGTCACCA 901 TGGTGCCTAG TGTTCCAGGA ATCCCAGGTC CTTCCTCTCC CCAACCAGTA CAGTCAGAAG 961 CAAAAATGAG ATTAAAAGCT GCTTTGACCC AGCACATCCT CCAGTTACCC AATGGTGATA 1021 CTGTCAACGT CATGGTAGCG CATTGATTAA GAACTCAGTC AGAAGGATCT CGACGCAGTC 1081 ATTAACGACA GCAAGCCACA TTCAACTAAC AGA //
Primer: T7 terminator Sequence file: RDB13434_A5Fbb.seq
>06321_13434_A5Fb_T7terminator_H08_23.ab1 1 ACGCATCTTT CGGGCTTTGT TAGCAGCCGG ATCCCCTCAA CTTCCTGAGG GCTGTGACTG 61 GGAGGAAGGA GCCATAACGA TCTGTGAAAG AGCAGGCTCT GTACTCTGGT CCGCCATCTG 121 GGTGAGGACT GAAGTGGCTA CAGCTTCTGC CTTGGAGGTT GAACTGACTC CATTGGATGT 181 GCTGACCGAA CTATGCTGTA TAGCTTCTGT ATGTGGACTA CTCGGCACTG AAATGTCTTC 241 TGAACTATCA TCTTTATCAG CAGTATGATA GCCAGATTTC TTCTGCATGG CGGTTACAGG 301 GCAATCTTTA TGAGCCAGAA GAAGCTGTTT CAGCTGTGCC ACTTCATTTC TCAGCAGGGT 361 GACTTCACTC TGCAGCTGAC CATTTAATGA ACTCAAGTCT TCAGCTTTCT TCTCTAAAGA 421 CTGAACCCAG ACTTTCCTTT TTTGTCGGCA TCTTGAAGCT GCTGCTCTAT TTCGCTCTAA 481 AACCTTTCTC CTTTTTTCAT CAGGATCTTC GTTAGCTGCT CTTCTCCGAC GACCACTTGT 541 ACTTTGGGTC TGTGGAGTTG TGTGAGCTGG AGAAGCCGGA GTTTCTGTAG TGGATGTGGC 601 TGGCTGTTGT AATGACTGCG GTCGAGATTC CTCTGACTGA GTCCTAACCA ATCCGCTACC 661 ATGACCTTTG ACAGTATCAC CATTGGTAAC TGGAGGATGT TGCTGGGTCA AAGCAGCTTT 721 TAATCTCATT TTTGCTTCTG ACTGTACTGG TTGGGGAGAG GAAGGACCTG GGATTCCTGG 781 AACACTAGGC ACCATGGTGA CTGGTCGAAC GAGTGGGACT GCAGCTGGAA CATGCACATT 841 AGAACTTGTA ATTGATGCAG GAATAGCAAC AGGCATGGTT TGTCCATTAG GAAGATGTAA 901 CAGAAGAGGA AATGGGGCCT GGTACAGGGA CAATTGGCCT GTTAGAGGAT GGTGCCTGGG 961 TGATTACAGT ACTTGAGGTT GGGTGAAAGG TACTGCCTGC TGAATAATTA CACTTTGAGT 1021 CAGAACTTTG TAAGCAGCAC ATTTGCGAAC CTGTAATGAT GGCTGACGGA ACATAGCTGA 1081 TGTGGCTGTG CAGCTTGTGC TATGGCTACG TACGTTCTCA ATTCACCTGG CTAAGTTTAG 1141 GAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Murata T., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971.

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl