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pDUAL2-FFH41

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter

Catalog number RDB06200
Resource name pDUAL2-FFH41
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364066.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06200 pDUAL2-FFH41 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06200z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14490_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14490_A6L7a.seq check
            >06200_14490_A6L7_nmt1Fw_A04_01_ABI24.ab1
                1 TGCAATTTAT TAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
               61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
              121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATAAA
              181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
              241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCGGA
              301 TATCATGGAT TATAAGGATG ATGACGATAA ACCCATGGAC TACAAGGACG ACGATGACAA
              361 ACATCACCAC CATCACCACT GAGATCTCGA CTCTAGAGGA TCCGGCGCGC CACTTCTAAA
              421 TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT AAGTTATAAA AAAAATAAGT
              481 GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA AATTCTTATT CTTGAGTAAC
              541 TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG AGGTCGCTCT TATTGACCAC
              601 ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT ACTAGTGCCT CTATTGATGC
              661 CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT TTGGAAGCTG ACGGTGTTCT
              721 TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT TGTCGTCCTG AGCAAGGTTT
              781 ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCCAACCC TTCGACCTTG CAACTTTGCC
              841 AGCAAGTCTT TAGTCAAGTA CAGCCCCTTT GAAGCCTGAA ATCGTTGAGG TGTCGATTTT
              901 TGTGTGTACG AGACTTACTG GAAGTGTACT TGGTGAGCGC ACTGAGACAC CGAATCGGTA
              961 TGCTATGACA CTGCCTACAG GTGAGGAGTT TCTCGATGCT CGATGCACGC GGGCCACTCA
            1021 TCATATCGAT CTCATCGATG ATAAGGTTTG CATAGAATCT
            //
            Primer: ADH-Forward
            Sequence file: RDB14490_A6L7b.seq check
            >06200_14490_A6L7_ADH-Forward_B04_04_ABI24.ab1
                1 TTCTCATTAA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
              121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
              181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
              241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
              301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
              361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
              421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
              481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
              541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
              601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
              661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
              721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
              781 ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT TTTAATTTGT ATTTTTTTGT
              841 AATTTTATTT CTCATTGTTT TACTTTAAAA TGAAATGCGA AATTAGAGAA AACTTTCAAC
              901 GAAATGTCAA AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT
              961 TCTCCGTGTA GATTCTTTTT TCTTCATTAA ACTCGACATG TAACTGCCCA ACTGAATTTT
             1021 TCTCTAGATG CTTAATAAAT TGAACTATGA ATATATAGCC AGTTGATAAT GTAACCTCTA
            1081 A
            //
            Primer: f1ori_R3
            Sequence file: RDB14490_A6L7c.seq check
            >06200_14490_A6L7_f1ori_R3_C04_07_ABI24.ab1
                1 GGCAGTGCGG CTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CGAGCTCGGG
              241 AGCGCTACCG TGAATGGGCT CAACCAAACA ATGAACCTTT TCTTCTGATT TTCCTACCAC
              301 ACCGGAAAGG GAGGCAGAAG GCAAAAGGCC CAAGCTACCA GGAATGACAG AAGCCTCATC
              361 TGAAATAATG TCACCAAACA AGTTGTCAGT CAAAACAACA CCGTTAAGTG TACGAGGGCT
              421 CTTGACCAAA AGCATGGCTG CGGAGTCAAT GAGCTGGTTT TTTAAGGTAA GGTGAGGATA
              481 TTCCTCCTTA AAAATCTTAG CTACAGTCTT GCGCCAAAGA CGAGAAGTTG CCAAAACATT
              541 AGCTTTGTCG AGTAATGTGA CGGGAGCAGG AGGGTTGGAA GTTTCAGCTA AGCGGCCGCG
              601 GGCCCGAGTC TAACTCCTTA ACCACTCGGA CATAGTGACT TATCTGACAC TCATTGTAAA
              661 TTAATATATA TATAGGCATT TTGTTTAGTT AAAGGTACTT AAGTAATTAG TATAAACGAA
              721 CCAATTTTAT AATCAGGAAG TTAAGTGAAT GGTAGCACAT GTCGTAAAAA TTGTGAATTT
              781 TTATTGAATA ATATTTTAAA TACAAGCCTT TCTAGACTAG GTATACTCAT AAACATATGA
              841 GCAAAAGGAT AGAGGAGATT ACATTGCATC TTCTACAAAT TTATTTATTG CCCTTTACTG
              901 AAAAATTAAA TAATGAGTAC TAAATGATAA AAAAGCGCTC AGTACAAGAA AGATTGCAAA
              961 AAATATTGCA TTCTTCATGA ATTAAAGGTG CATATACGCA TATTGAAGGT ATTAGTTACT
             1021 AAACAGGCAG GTTAGCGAAA TGTTAGCATT ATATCGCAGA ACATGTGACA GTAATAAAAA
            1081 CTG
            //
            Primer: Reverse2
            Sequence file: RDB14490_A6L7d.seq check
            >06200_14490_A6L7_Reverse2_D04_10_ABI24.ab1
                1 CAGGCATGAT TACGCCAAGC TTGTGATATT GACGAAACTT TTTGACATCT AATTTATTCT
               61 GTTCCAACAC CAATGTTTAT AACCAAGTTT TATCTTGTTT GTCTACATGG TATTTTACAT
              121 TCATCTACAT ACATCTTTCA TTGGCTTTGT ACATAGTTAT CATTACAAGT CTAAAAAAAT
              181 TCACTCTTTT CTTATTCAAT GTCAATCCAA GAGAAAAGAT TGTGGTAATG TTGTAGGAGC
              241 ATGTTTAATA AATTACTATA GCAAATTACT TTTTATTCCC AAGGTGTTTA TCTATAATAG
              301 TTAATATTTT AGTCGCTACA TAAAATTTTA CCAAAGAGTA CTTGTATACT AATTCTAAAT
              361 GCCTTCTGAC ATAAAACGCC TAGGAAAACA AACGCAAACA AGGCATCGAC TTTTTCAATA
              421 ACCAACCAAA AAAATTTTAC ATTAGTCTTT TTTTAATGCT GAGAAAGTCT TTGCTGATAT
              481 GCCTTCCAAC CAGCTTCTCT ATATCTCTTG GCTTCGACAA CAGGATTACG ACCAGCTCCA
              541 TAGACTCCAC GACCAACAAT GATGATGTCG CTACCGCAGT TTACAATCAC TTCTTCAGGA
              601 GTACGATATT GCTGTCCCAG CCCGTCTCCT TTAACATCCA AGCCGATACC AGGGGACATA
              661 GTTATGTAGT CGCTTTGAAG GTTAGGAAAT CGACGACCAG CTATAAAGCC AAAGCAAAAA
              721 TCGGTATGCT TCTCAAACCA TTCTAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
              781 CTTTGGAAGA CATTTCAGCC AAAAGCAGGA GACACGTCCA AAGGTAAACC ACTTCTTTGA
              841 GGCTTGTATA TACCCTCGCT GCACTGTATG GCCATTTGTG ATATGAGCCC AGAGCATTTT
              901 GTACAACCAG ATGCATATTT GTAGCCTGGA CGTAATTGCA TGTTCTGGCA ATGCCGAATC
            961 TCAGATAGGA CGATGCTTAC CTAGGCACAG TTTCACGAAT CCTGTCGAAT C
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl