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pDUAL2-FFH1

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06199
Resource name pDUAL2-FFH1
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364065.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06199 pDUAL2-FFH1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06199z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14529_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14529_A6L7a.seq check
            >06199_14529_3_A6L7_nmt1Fw_G04_07_ABI08.ab1
                1 TGAAATTAAT AAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
               61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
              121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA
              181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
              241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
              301 CGGATATCAT GGATTATAAG GATGATGACG ATAAACCCAT GGACTACAAG GACGACGATG
              361 ACAAACATCA CCACCATCAC CACTGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC
              421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT
              481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG
              541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA
              601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT GCCTCTATTG
              661 ATGCCTATGG AACCCCTTTG ACTGACGAGA CTGTGAAGGC TTGTTTGGAA GCTGACGGTG
              721 TTCTTTTGGG TGCCGTTGGT GGTCCTGAAT GGACCAACCC CAATTGTCGT CCTGAGCAAG
              781 GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTCGACCT TGCAACTTTG
              841 CCAGCAAGTC TTTAGTCAAG TACAGCCCTT TGAAGCCTGA AATCGTTGAA GGTGTCGATT
              901 TTTTGTGTTG TACGAGAACT TTACTGGAGG TTGTTACTTT GGGTGAGCGC ACTGAGGACA
              961 ACGGGATCGG GGTTATGCTA TGGACACTTT GGTCCTTTAC AGTTTGGCAA GGAAGTTTCT
             1021 CGTATGCTCG TTTGCGCCGC GACCATCCTT CCAATTCATT AATCGAGTTC GTCATCAGAT
            1081 GATTTTAGAC GGAT
            //
            Primer: ADH-Forward
            Sequence file: RDB14529_A6L7b.seq check
            >06199_14529_3_A6L7_ADH-Forward_H04_08_ABI08.ab1
                1 ACCTCTCTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
               61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
              121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
              181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
              241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
              301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
              361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
              421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
              481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
              541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
              601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
              661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
              721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTATG GGCAAGCGAG ACAGAATGGG
              781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT
              841 AATTTTAATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA ACTTTCACGA
              901 ATGTCAAAAT AAGCTTCAGC AAAAATATAA CAATTTTAGG AAAGGCGATC AGCCACTTAT
              961 TCTCGGTGGT AGATTCCTTT TCCTCCATAA TTCGAACAAT GTACTGGCCG ATGGATTTTC
            1021 TCTTAGAATG CCTAATAATT GGTACTATGA TGATAGCCAG TTGATAG
            //
            Primer: f1ori_R3
            Sequence file: RDB14529_A6L7c.seq check
            >06199_14529_3_A6L7_f1ori_R3_A05_01_ABI08.ab1
                1 GCCGATGGGT GTCCTTTGCG TATGCGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT
               61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC
              121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
              181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC
              241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT
              301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC
              361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA
              421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA
              481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA
              541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG
              601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT
              661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA
              721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG
              781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA
              841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT
              901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAAAG CGCTCAGTAC AAGAAAGATT
              961 GCAAAAATAT TGCATTCTTT CATGAATTAA GTTGGCATAT AAGCATAATT GAAGGTTAAT
             1021 AGTTACTAAA ACAGCAGTTA GCCGAAATAT AGGATTAATA TTCGCAGAAA TTGTGCACAT
            1081 AAGTTGAAGG AAATGTGTAC GA
            //
            Primer: Reverse2
            Sequence file: RDB14529_A6L7d.seq check
            >06199_14529_3_A6L7_Reverse2_B05_02_ABI08.ab1
                1 AGGGCCATGC ATTCGCCAAG CTTGTGATAT TGACGAAACT TTTTGACATC TAATTTATTC
               61 TGTTCCAACA CCAATGTTTA TAACCAAGTT TTATCTTGTT TGTCTACATG GTATTTTACA
              121 TTCATCTACA TACATCTTTC ATTGGCTTTG TACATAGTTA TCATTACAAG TCTAAAAAAA
              181 TTCACTCTTT TCTTATTCAA TGTCAATCCA AGAGAAAAGA TTGTGGTAAT GTTGTAGGAG
              241 CATGTTTAAT AAATTACTAT AGCAAATTAC TTTTTATTCC CAAGGTGTTT ATCTATAATA
              301 GTTAATATTT TAGTCGCTAC ATAAAATTTT ACCAAAGAGT ACTTGTATAC TAATTCTAAA
              361 TGCCTTCTGA CATAAAACGC CTAGGAAAAC AAACGCAAAC AAGGCATCGA CTTTTTCAAT
              421 AACCAACCAA AAAAATTTTA CATTAGTCTT TTTTTAATGC TGAGAAAGTC TTTGCTGATA
              481 TGCCTTCCAA CCAGCTTCTC TATATCTCTT GGCTTCGACA ACAGGATTAC GACCAGCTCC
              541 ATAGACTCCA CGACCAACAA TGATGATGTC GCTACCGCAG TTTACAATCA CTTCTTCAGG
              601 AGTACGATAT TGCTGTCCCA GCCCGTCTCC TTTAACATCC AAGCCGATAC CAGGGGACAT
              661 AGTTATGTAG TCGCTTTGAA GGTTAGGAAA TCGACGACCA GCTATAAAGC CAAAGCAAAA
              721 ATCGGTATGC TTCTCAAACC ATTCTAAGGT TTTCTCTGTG TAGGAACCAG TAGCCAAAGA
              781 GCCTTTGGAA GACATTTCAG CCAAAAGCAA GAGACCACGT CCCAAAGGTA AACCAACTTC
              841 TTTGAGGCCT TGTATAATAC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA
              901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTTT GACGTATTTT CCCAATGTCT
              961 GCGAATTTGG CGATCCTCAA GATAAGAAAC GATGCTTTTT TACCTAAGGC AACAAGTTTT
             1021 TCTACCATAT TCCTTGTTCG GAATCCTCGA CCAACCGTCG ATATGGTCGT GATTAGCCAG
            1081 GAGTATGGTT CCGT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl