Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-HFG41

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14462_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14462_A6L7a.seq
>06197_14462_A6L7_nmt1Fw_G02_20_ABI24.ab1     1 AGCATTTTAT TAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAA   181 AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA   241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCTA   301 TGCATCACCA CCATCATCAT ATGGATTATA AGGATGATGA CGATAAACCC ATGGGTAAAG   361 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG   421 GGCACAAATT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC   481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT   541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT   601 TCAAGAGTGC CATGCCCGAA GGCTATGTAC AGGAAAGAAC TATATTTTTC AAAGATGACG   661 GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATAGAATCG   721 AGTTAAAAGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA TTGGAAATAC   781 AACTATAACT CACACAATGT ATACATCATG GCAGACAAAC AAAAGAATGG AATCAAAGTT   841 TAACTTCAAA ATTAGACACA ACATTGAAGA TGGGAAGCGT TTCAACTAGC AGACCATTAT   901 CAACAAAAAT ACTCCAATTG GCGATGGGCC CTGTCCTTTT ACCAGACACC ATTACCTGTC   961 CACACATCTG CCTTTCGAAA GATCCCCACG AAAAAGAAGA GAACACATGG TCTTCTTGAG  1021 TTTGTAACAG CTGCCTGGGA ATTAACACAT TGGCCATTGA ATGGACTATA CAAGCATTCA 1081 TGAATCTCGA ACTTCTTAGA GAGGGGACTT CCGGGC //
Primer: ADH_rev Sequence file: RDB14462_A6L7b.seq
>06197_14462_A6L7_ADH_rev_H02_23_ABI24.ab1     1 GGGGAGTCTC AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA    61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT   121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT GTATAGTTCA   181 TCCATGCCAT GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC   241 TTTTCGTTGG GATCTTTCGA AAGGGCAGAT TGTGTGGACA GGTAATGGTT GTCTGGTAAA   301 AGGACAGGGC CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCTT   361 CCATCTTCAA TGTTGTGTCT AATTTTGAAG TTAACTTTGA TTCCATTCTT TTGTTTGTCT   421 GCCATGATGT ATACATTGTG TGAGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT   481 CCATCTTCTT TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA   541 AACTTGACTT CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC   601 TGTACATAGC CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCTGTTTCAT GTGATCTGGG   661 TATCTTGAAA AGCATTGAAC ACCATAACAG AAAGTAGTGA CAAGTGTTGG CCACGGAACA   721 GGTAGTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTCCGTATG TTGCATCACC   781 TTCACCCTCT CCACTGACAG AAAATTTGTG CCCATTAAAC ATCACCATCT AATTCAACAA   841 GAAATTGGGA CAACTCCCAG TGAAAAGTTT CTTCTCCTTT ACCCATGGGG TTTTATCGTC   901 ATCATCCCTT ATAATCCATT ATGATGATGG TGGTGATGCC ATAGGATCCA AGGGCCTGTC   961 GACGCTAGGC TTTAACAAAA GCGACCTATA GGTCAGGAAG TGGAGTATGA GATGCAGATT 1021 AGTCACTATT TCCAATTGGA //
Primer: ADH-Forward Sequence file: RDB14462_A6L7c.seq
>06197_14462_A6L7_ADH-Forward_A03_03_ABI24.ab1     1 TAGGCACTTA ATGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT    61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG   121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT   181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA   241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT   301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC   361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT   421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT   481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT   541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT   601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTTCTA   661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA   721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAAGTA GGGCAAGCGA GACAGAATGG   781 GATACAAGGG CATCGTCTAT TAATTATTAG CTAAAAATGT ATTTTAATTT GTATTTTTGT   841 ATTTTATTTT CTCAATGTTT ACTAAAATGA ATGCGACTAG AAGAAAACTT TCACGAATGT   901 CAAAAATAAG CTCAGCAAAA TTATACAATT TAAACAAGCG ATCAGCACTT ATTCTCGGTT   961 GTAGAATTCT TTTCTACAAT AATCGACATG TTACTGGCAA TTGGAATGTC CCTTGATGCC 1021 TTATAAATGA AGCCTAATG //
Primer: f1ori_R3 Sequence file: RDB14462_A6L7d.seq
>06197_14462_A6L7_f1ori_R3_B03_06_ABI24.ab1     1 GCGATTGCGG CTTGCGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG    61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC   121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT   181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG   241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA   301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT   361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC   421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT   481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT   541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC   601 GGGCCCGAGT CTAACTCCTT AACCACTCGG ACATAGTGAC TTATCTGACA CTCATTGTAA   661 ATTAATATAT ATATAGGCAT TTTGTTTAGT TAAAGGTACT TAAGTAATTA GTATAAACGA   721 ACCAATTTTA TAATCAGGAA GTTAAGTGAA TGGTAGCACA TGTCGTAAAA ATTGTGAATT   781 TTTATTGAAT AATATTTTAA ATACAAGCCT TTCTAGACTA GGTATACTCA TAAACATATG   841 AGCAAAAGGA TAGAGGAGAT TACATTGCAT CTTCTACAAA TTTATTTATT GCCCTTTACT   901 GAAAAAATTA AATAATGAGT ACTAAATGAT AAAAAGCGCT CAGTACAAGA AAGATTGCAA   961 AAATATTGCA TTCCTTCATG AAATTAAAGT TGCATATACG CATATTGAAA GTAATAGTAC  1021 TTAAAACAGC AGTTTAGCGA AAATTAATTA GGATTATATT CGGCAAGACA TGTGACAGTC 1081 AATTTAAAAA ATTGTAGCAA TTTTTTTTAC //
Primer: Reverse2 Sequence file: RDB14462_A6L7e.seq
>06197_14462_A6L7_Reverse2_C03_09_ABI24.ab1     1 CAGAATCATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT    61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC   121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC   181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT   241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT   301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC   361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC   421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC   481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA   541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT   601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT   661 TATGTAGTCG CTTTGAAGGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC   721 GGTATGCTTC TCAAACCATT CTAAGGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC   781 TTTGGAAGAC ATTTCAGCCA AAAGCAAGAG ACCACGTCCC AAAGGTAAAC CAACTTCTTT   841 GAGGCCTTGT ATAATACCCT CGCCCTGGCA CTGTATGGCA AATTTGTGAT ATGAGCCCAA   901 GAAGCAATTT TGTACACACC AGATGCATAT TGTAGCTTGA CGGTATTTTC AATGTCTGCG   961 ATTGCGATCC TCAAAGAATA GGAAACCGAT GCCTTTTACT ACGTCACAGT TTTCTAACAT  1021 ATCTGATTCG AATCCTCGGA CACCGTCGAA TGTGATCTGA ATACTCAGGA ACTAGGGGAT 1081 CA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl