Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL2-HFG1

S. pombe 5 ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14461_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14461_A6L7a.seq
>06196_14461_A6L7_nmt1Fw_B02_05_ABI24.ab1     1 CGGCATTATT AATCAGGAAA CGTAACTCTC GGCTACTGGA TGGTTCAGTC ACCCAACGAT    61 TACTGGGGAG AGAAAACAGG GCAAAAGCAA AGCTTAAAGG AATCCGATTG TCATTCGGCA   121 ATGTGCAGCG AAACTAAAAA CCGGATAATG GACCTGTTAA TCGAAACATT GAAGATATAT   181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT   241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC   301 TATGCATCAC CACCATCATC ATATGGATTA TAAGGATGAT GACGATAAAC CCATGGGTAA   361 AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA   421 TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC   481 CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC TTGTCACTAC   541 TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC AGCATGACTT   601 TTTCAAAGAG TGCCATGCCC GAAGGCTATG TACAGGAAAG AACTATATTT TTCAAAGATG   661 ACGGGGAACT ACAAGACACG TGCTGAAGTC AAAGTTTTGA AGGTGATACC CTTGTTAATA   721 GAAATCGAGT TAAAAGGTAT TTGAATTTTA AAGAAAGATG GGAAACATTT CTTGGGACAC   781 AAATTGGAAT ACAACTATTA ACTCACCACA ATTGTATACC ATCAATGGTC AGACAAAACC   841 AAAAGAATTG GAATCAAAAG TTAACTTTCC AAAATTGAGT CACAACATTT GAAGAATGGA   901 AAGCCGTTGC AACCTAGCAA GACCATTTAT CCACCAAAGT ACTTCGAATT GGGCGAATGC   961 GCCCTGGTAC CTTTTTAACA AAACAACCAT TTTACCCTGA CCCATAGCAA TGCTGGCCAT 1021 TACAAAAGAT ATCCCAAACG AAAAAAAAAG CACTACTGAG GTC //
Primer: ADH_rev Sequence file: RDB14461_A6L7b.seq
>06196_14461_A6L7_ADH_rev_C02_08_ABI24.ab1     1 GCGAGAATCT CAGAATAAGA TTTTCGTTTT AAACCTAAGA GTCACTTTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCTT   121 ATTTAGAAGT GGCGCGCCGG ATCCTCTAGA GTCGAGATCT CATGATATCT TTGTATAGTT   181 CATCCATGCC ATGTGTAATC CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC   241 TCTTTTCGTT GGGATCTTTC GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA   301 AAAGGACAGG GCCATCGCCA ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC   361 TTCCATCTTC AATGTTGTGT CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT   421 CTGCCATGAT GTATACATTG TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT   481 TTCCATCTTC TTTAAAATCA ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT   541 CAAACTTGAC TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT   601 CCTGTACATA GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG   661 GGTATCTTGA AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA   721 CAGGTAGTTT TCCAGTAGTG CAAATAAATT TAAAGGGTAA GTTTTCCGTA TGTTGCATCA   781 CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCATC TAAATTCAAC   841 AAGAATTGGG ACACTCCAGT GAAAAGTTCT TTCTCCTTTA CCCATGGGTT TATCGTCATC   901 ATCCCTTATA ATCCATATGA TGATGGTGGG TGATGCATAA GATCCAGGCC CTTGTCGACG   961 CTAGCTTTAA ACAAAGCGAC TATAGTCAGA AAGTGAGATT GAGATGAAAT ATGACTTCCA 1021 CTATCATTGG ATGGAATATG CAGGAATCCG TCTTCCTTAA AAATCGTCCA AGT //
Primer: ADH-Forward Sequence file: RDB14461_A6L7c.seq
>06196_14461_A6L7_ADH-Forward_D02_11_ABI24.ab1     1 AACCTCCTCT TAATTGACAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT    61 ACTAGTGCCT CTATTGATGC CTATGGAACC CCTTTGACTG ACGAGACTGT GAAGGCTTGT   121 TTGGAAGCTG ACGGTGTTCT TTTGGGTGCC GTTGGTGGTC CTGAATGGAC CAACCCCAAT   181 TGTCGTCCTG AGCAAGGTTT ATTGAAGCTT CGTAAGAGTA TGGGTGTTTG GGCCAACCTT   241 CGACCTTGCA ACTTTGCCAG CAAGTCTTTA GTCAAGTACA GCCCTTTGAA GCCTGAAATC   301 GTTGAAGGTG TCGATTTTTG TGTTGTACGA GAACTTACTG GAGGTTGTTA CTTTGGTGAG   361 CGCACTGAGG ACAACGGATC GGGTTATGCT ATGGACACTT GGCCTTACAG TTTGGAAGAA   421 GTTTCTCGTA TTGCTCGTTT GGCGGCCGCG GGCCCAACCT TCCAATTCAT TAAATCGAGT   481 CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT AAGTAAGAGA AGTTGTTAGT   541 GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG ATCTCTTCTC CATTGTTTCC   601 TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT ATTTTCTGAT TATTTTTTTC   661 TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG TAAAATCGGG GTATGGTTAG   721 TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT AGGGGCAAGC GAGACAGAAT   781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG TATTTTAATT TGTATTTTTT   841 TGTAATTTTA TTTCTCATTG TTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC   901 GAAATGTCAA AAATAAGCTC AGCAAAATAT ACAATTTTAC GGGAAAGCGA TCAGCAACTT   961 TATTCTCCGT TTGTAGATTC TTTTTTCTTC ATAAATTCCC AACATGTAAA CTGCCCATTT  1021 GATTTTTTCT CTAGATGCCT AATAAATTGA AACTTATGTA TAATAATTAC ACAAGGTTTT 1081 GATAATGTAA ACTCTTACTC AGAAAAGGA //
Primer: f1ori_R3 Sequence file: RDB14461_A6L7d.seq
>06196_14461_A6L7_f1ori_R3_E02_14_ABI24.ab1     1 GGGATGCTCT GAGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT    61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC   121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC   181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCG AGCTCGGGAG   241 CGCTACCGTG AATGGGCTCA ACCAAACAAT GAACCTTTTC TTCTGATTTT CCTACCACAC   301 CGGAAAGGGA GGCAGAAGGC AAAAGGCCCA AGCTACCAGG AATGACAGAA GCCTCATCTG   361 AAATAATGTC ACCAAACAAG TTGTCAGTCA AAACAACACC GTTAAGTGTA CGAGGGCTCT   421 TGACCAAAAG CATGGCTGCG GAGTCAATGA GCTGGTTTTT TAAGGTAAGG TGAGGATATT   481 CCTCCTTAAA AATCTTAGCT ACAGTCTTGC GCCAAAGACG AGAAGTTGCC AAAACATTAG   541 CTTTGTCGAG TAATGTGACG GGAGCAGGAG GGTTGGAAGT TTCAGCTAAG CGGCCGCGGG   601 CCCGAGTCTA ACTCCTTAAC CACTCGGACA TAGTGACTTA TCTGACACTC ATTGTAAATT   661 AATATATATA TAGGCATTTT GTTTAGTTAA AGGTACTTAA GTAATTAGTA TAAACGAACC   721 CAATTTTATA ATCAGGAAGT TTAAGTGAAT GGGTAGCACA TGTCGTAAAA ATTGTGAATT   781 TTTATTGAAT AATATTTTTA AATACAAGCC CTTTTCTAGA CTAGGTATAC TCATAAACAT   841 ATGAGCAAAA TGATAGAGGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT   901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAGCC GCCCTCAGTT ACAAGAAAGA   961 TTGCAAAAAT ATTGCCATCG TCATGGACTC AAGGTGCCAT ATAGCCATAT TTGAAGATTG 1021 ATAGTACTAA CTGCAGTTAG CGCG //
Primer: Reverse2 Sequence file: RDB14461_A6L7e.seq
>06196_14461_A6L7_Reverse2_F02_17_ABI24.ab1     1 CGTACTCATT ACGCCAGCTT GTGATATTGA CGAAACTTTT TGACATCTAA TTTATTCTGT    61 TCCAACACCA ATGTTTATAA CCAAGTTTTA TCTTGTTTGT CTACATGGTA TTTTACATTC   121 ATCTACATAC ATCTTTCATT GGCTTTGTAC ATAGTTATCA TTACAAGTCT AAAAAAATTC   181 ACTCTTTTCT TATTCAATGT CAATCCAAGA GAAAAGATTG TGGTAATGTT GTAGGAGCAT   241 GTTTAATAAA TTACTATAGC AAATTACTTT TTATTCCCAA GGTGTTTATC TATAATAGTT   301 AATATTTTAG TCGCTACATA AAATTTTACC AAAGAGTACT TGTATACTAA TTCTAAATGC   361 CTTCTGACAT AAAACGCCTA GGAAAACAAA CGCAAACAAG GCATCGACTT TTTCAATAAC   421 CAACCAAAAA AATTTTACAT TAGTCTTTTT TTAATGCTGA GAAAGTCTTT GCTGATATGC   481 CTTCCAACCA GCTTCTCTAT ATCTCTTGGC TTCGACAACA GGATTACGAC CAGCTCCATA   541 GACTCCACGA CCAACAATGA TGATGTCGCT ACCGCAGTTT ACAATCACTT CTTCAGGAGT   601 ACGATATTGC TGTCCCAGCC CGTCTCCTTT AACATCCAAG CCGATACCAG GGGACATAGT   661 TATGTAGTCG CTTTGAAAGT TAGGAAATCG ACGACCAGCT ATAAAGCCAA AGCAAAAATC   721 GGTATGCTTC TCAAACCATT CTAAAGTTTT CTCTGTGTAG GAACCAGTAG CCAAAGAGCC   781 TTTGGGAAGA CATTTCAGCC CAAAAGCAAG AGACCACGTC CCAAGGTAAA CCAAACTTCT   841 TTTGAGGCCT TGTATATACC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA   901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTGA CGTATTTCAT GTCTGCGATT   961 GCGATCTTCA AAGAATAAGA AAACGAATGC TTTTACTTAA GCCACAGTTT TCTACGATAT 1021 CTGCTCGTAT CTCGACACGG TCAATTGGTG CCTTGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl