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pDUAL2-HFF1

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06193
Resource name pDUAL2-HFF1
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364059.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06193 pDUAL2-HFF1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06193z.seq

            Original reference

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14487_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14487_A6L7a.seq check
            >06193_14487_A6L7_nmt1Fw_A09_03_ABI24.ab1
                1 ATGGCATTTT ATTAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
              241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
              301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
              361 GACTACAAGG ACGACGATGA CAAAGATATC ATGAGATCTC GACTCTAGAG GATCCGGCGC
              421 GCCACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA
              481 AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA
              541 TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT
              601 CTTATTGACC ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTGC
              661 CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT GTGAAGGCTT GTTTGGAAGC
              721 TGACGGTGTT CTTTTGGGTG CCGTTGGTGG TCCTGAAATG GACCAACCCC CAATTGTCGT
              781 CCTGAGCAAG GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTTCGACC
              841 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTTGAAGCCC TGAAATCGTT
              901 GGAAGTGTCG ATTTTTTGTG TTGTACGGAG AACTTACTGG AGTTGTTTAC TTTGTGGGAG
            961 CGCCCTTGAG
            //
            Primer: ADH-Forward
            Sequence file: RDB14487_A6L7b.seq check
            >06193_14487_A6L7_ADH-Forward_C04_03_ABI08.ab1
                1 TACGGTTTTA TTGAACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
               61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
              121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
              181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
              241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
              301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
              361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
              421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
              481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
              541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
              601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
              661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
              721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG
              781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTTG
              841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAAATGCGA AATTAGAGAA AACTTCAACG
              901 AAATGTCAAA ATAAGCTCAG CAAAATATAC AATTTTAGGG AAAGCGATCA GCAACTTTAT
              961 TCTCCGTTGT AGATTCTTTT TCTTCATAAA ATTTCCACAA TGTAACCTGG CCAAATTGAT
             1021 TTTTTTCTTC TAGATGCTTA TACTTGGAAC CTTACTGCAA TATACTAACA CCAGGTTTTG
            1081 GCATAAAGTG GTCAC
            //
            Primer: f1ori_R3
            Sequence file: RDB14487_A6L7c.seq check
            >06193_14487_A6L7_f1ori_R3_C09_09_ABI24.ab1
                1 GCATGGCGGC TTTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
               61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
              241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
              301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
              361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
              421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
              481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
              541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
              601 GGGCCCGAGT CTAACTCCTT AAACCACTCG GACATAGTGA CTTATCTGAC ACTCATTGTA
              661 AATTAATATA TATATAGGCA TTTTTGTTTA GTTAAAGGTA CTTAAGTAAT TTAGTATAAA
              721 CGAACCAATT TTATAATCAG GAAAGTTTAA GTGAATGGGT AGCACATGTC GTAAAAATTG
            781 TGGAATTTTT TATTTGAAAT AAAATTTTAA ATACAAAACC TTTTCTAAAA CCAGGTAT
            //
            Primer: Reverse2
            Sequence file: RDB14487_A6L7d.seq check
            >06193_14487_A6L7_Reverse2_D09_12_ABI24.ab1
                1 CAGTCCTGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
               61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
              121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
              181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
              241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
              301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
              361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
              421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
              481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
              541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
              601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
              661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
              721 CGGTATGCTT CTCAAACCAT TCTAAGGGTT TTCTCTGTGT AGGAACCAGT AGGCCAAAGA
              781 GCCTTTGGGA AGACATTTCA GCCAAAAGGC AAGAGACCAC GTCCCAAAGG TAAAACCAAA
              841 CTTTCTTTGG AAGGCCTTGT ATAATACCCT TCGCCCTGGG CAACTGTATG GGCAATTTGG
              901 TGGAATATGG AGGCCCAAAG AAAGCAATTT TGGTACACAG CAGGATGGCA TATTTGGTAG
              961 GCTTGGACGG GTAATTTTCC ATGGTTCTGG CGAAATTTGG CGGATCCTCA AAGAATAGAA
             1021 ACGGATGGCC TTTACCTTAG CACCAGGTTT TCTACATATC TGGTCGGAAG TCTTCGGAAC
            1081 AAAGCGTCCA ATGTGGTGGT CCTTTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.05.18

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