Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-YFH1

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13523_A5Ipp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536) Region: insert 5'(nmt1 pro 5') Sequence file: RDB13523_A5Ipf.seq
>06191_13523_B2DsS_pUC_CAPbs_up_F_E01_05_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTAGCTCAC TCNATTAGGC    61 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA   121 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGCC AAGCTTGCAT GCCTGCAGGT   181 CGATCGACTC TAGAGGATCA GAAAATTATC GCCATAAAAG ACAGAATAAG TCATCAGCGG   241 TTGTTTCATT TCCTATATTT TTTTTTTATT TTTTTATTTT TTAATAAGGG AAAATTTAAC   301 GTCTAAGGAT ACAGAAGATT GTTAGCACAT TAAAGTAATA AAGGCTTAAG TAGTAAGTGC   361 CTTAGCATGT TATTGTATTT CAAAGGACAT AATCTAAAAT AATAACAATA TCATTTCTCA   421 CAAGTTATTC AATTTTCTTT TTTTTTTCTA ATAATATCAA GAATGTATTA TTTGTTTGAC   481 ATAAGTCAAC TAATTTATTT AATATGCTGG ATTAATCTTG CAGACATGTA AATTAACAAG   541 TTTTAGTCAA ATAACGTTGA AGTTTCNATG AACTCNAATA ATTTCTCTTT TTTTTTATAT   601 AACNNTAATC TGATTTATAT TTTCCGCANG GATNNNNTGN AGTTATGACA TTTGGNNTNN   661 NTCACTTANN NNCNNGGTCG CCAAATNATA CNAAATCANC GNTANAAAAC NNNNNNNTTT 721 NGNNAANNAA NNNNNNNNNN //
Primer: ADHrev (Pr0048) Region: ADH ter,insert 3'(6xHis,FLAG,YFP 3') Sequence file: RDB13523_A5Ipg.seq
>06191_13523_A5Ip_ADHrev_E05_14.ab1     1 NNNNNNNNNN NNNNNNNANT TTCGTTTTNN ACCTAAGAGT CACTTTAAAA TTTGTATACA    61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCTTAT   121 TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA GTGGTGATGG TGGTGATGTT   181 TGTCATCGTC GTCCTTGTAG TCCATGGGCT TGTACAGCTC GTCCATGCCG AGAGTGATCC   241 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC   301 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA   361 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC   421 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT   481 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA   541 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG   601 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA   661 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AANCACTGCA   721 GGCCGTANCC GAANGTGGTC ACGANGGTGG GCCAGGGCAC GGGCAGCTTG CCGGNGGTGC   781 AGATGAACTT CANGGTCAGC TNGCCGNNNN GGCNTCGCCC TCGCCCNCGC CGGNNNNGCN   841 NAACTTGNGG NCNNTTACGT NNNNNNCCNN CTCNNNCANN NNGGGNANNN NCNNNNNNNN 901 NN //
Primer: nmt1Fw (Pr0004) Region: insert mid(nmt1 pro 3',YFP 5') Sequence file: RDB13523_A5Iph.seq
>06191_13523_A5Ip_nmt1Fw_D05_11.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNTAACTC TCGGCTACTG GNNGGTTCNG TCACCCAACG    61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG   121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT   181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT   241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT   301 CCGGATATCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC   361 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT   421 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC   481 TGGCCCACCC TCGTGACCAC CTTCGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC   541 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AANGCTACGT CCAGGANCGC   601 ACCATCTTCT TCAAGGACGA CGGCAACTAC NAGACCCGCG CCGANGTGAA GTTCGAGGGC   661 GACACCCTGG TGAACCGCAT CGAGCTGAAN GGCATCGACT TCAAGGANGA NGGCAACNTC   721 CTGGGGCACN AGCTGNANTA NNNCTACNNC AGCCNCNACG TCTATATCAT GGNNGANAAN   781 CAGAANNACG GNNTCNNNNN GNNNTTNNNN NNNGNNNNNA NATCNANNAN NNNNNNNNGC 841 NNNNNNNNNA NNNNTNNNNN NNNNN //
Primer: URA4pro_F (Pr0371) Region: ura4+ mid Sequence file: RDB13523_A5Ipi.seq
>06191_13523_B2DsS_URA4pro_F_C01_03_ABI08.ab1     1 NNNNNNNNNN CCNNNNNNNN ACCTCTTTTT TGCTTGGATC GAAATTAAAG GTTTAAAAGC    61 AAAGTTATGG ATGCTAGAGT ATTTCAAAGC TATTCAGCTA GAGCTGAGGG GATGAAAAAT   121 CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT GTCAGTCGCG   181 GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGTAG ATAAAATTGG ACCCTATGTC   241 TGTGTTATCA AGACACATAT TGACGTTGTC GAGGATTTCG ACCAGGATAT GGTAGAAAAA   301 CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA ATTCGCAGAC   361 ATTGGAAATA CCGTCAAGCT ACAATATGCA TCTGGTGTGT ACAAAATTGC TTCTTGGGCT   421 CATATCACAA ATTGCCATAC AGTGCCAGGC GAGGGTATTA TACAAGGCCT CAAAGAAGTT   481 GGTTTACCTT TGGGACGTGG TCTCTTGCTT TTGGCTGAAA TGTCTTCCAA AGGCTCTTTG   541 GCTACTGGTT CCTACACAGA GAAAACCTTA GAATGGTTTG AGAAGCATAC CGATTTTTGC   601 TTTGGCTTTA TAGCTGGTCG TCGATTTCCT AACCTTCNAA NCGACTACNT AACTATGTCC   661 CCNGNNATCN GCTTNNNNGN NAANNNNANG GNCTGGGNNN GCNANATCNN ACTNNNNNAN 721 NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl