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pDUAL

S. pombe pDUAL series backbone vector

Catalog number RDB06178
Resource name pDUAL
Clone info. S. pombe pDUAL series backbone vector.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364045.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              
             
            Sequence RDB06178z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB06178 pDUAL DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pDUAL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06178).

            Reference section:

            Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13001_A4Js.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Reverse2
            Sequence file: RDB13001_A4Jsa.seq check
            >06178_13001_A4Js_Reverse2_A03_03.ab1
                1 ATTCCATGAA TTCGCCAAGC TTGCATGCCT GCAGGTCGAC TCTAGAGGAT CCGGCGCGCC
               61 ACTTCTAAAT AAGCGAATTT CTTATGATTT ATGATTTTTA TTATTAAATA AGTTATAAAA
              121 AAAATAAGTG TATACAAATT TTAAAGTGAC TCTTAGGTTT TAAAACGAAA ATTCTTATTC
              181 TTGAGTAACT CTTTCCTGTA GGTCAGGTTG CTTTCTCAGG TATAGTATGA GGTCGCTCTT
              241 ATTGACCACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA CTAGTAAGAT
              301 GATTCTTTTA TCGTCGATAA CGATGAGGAT GTAGACGATT ATGATGAGAG TGATTAGCTT
              361 CTAAAATTTG GTGTTTTCAA ACGCTATTTA CAGTACAATC TGAAAAAATT GGATGAACAT
              421 TGTAAATGGT AGGATACGAG TTCCAATTAA TGTATTTTTT AAGTATTACA CTTAGCTTAT
              481 TAATTTTATA TATACATTCT ACCTCTGTGG GTTTAAAGAC AACTCGTACA CATACTCAAA
              541 TTATTTATGA ACATTATAAT GCATATTCGT ACTGTCTATT AAACATTTTT TTGACATTAA
              601 AAATTTCGTT TACTAACGTA GAAAGCGCTG ATAATTGAGG CTACTATTTT AATATCTAGA
              661 AACAAAGATT CATTAATTAA TTAAGTAAGC TCCAGGATAC TTGTATATTT CGTTAAAAAA
              721 GCGCAATTTC AACAATTCCT ATGAACATCC ATGATTTCAT CACAAAATCA AACATATCAG
              781 GATTTAACGT GAATTATTTG CTTTCTTGTG ATTTTCATCT GATTTCTGGT CATTTACGTT
              841 ACTGTAAACA AGCTTTTTAA AAAAAAGGAT TTTTTACGCA ATATTATCGA TATCCCAATC
              901 TGTAGTATAA AAAGTGCAAC AGTGTTGAGT TTCCCGAAAC CAGGCATTTT TGCGGTAGGG
              961 TCTGAAACTT TCACGAAACC ATTTCACAAC AAAACTTCTT CAACAACATG TGTGCAAAGA
             1021 AATCGTCGTC TTACCAGGAG ACCATATTGG CCCTGAAATT GTTGCTTCTG CCTTTGGAGG
             1081 TTTTGAAGGT CGGTTGAGAG AGCGACCCTG ACTAAACTCG AGCTTGAGAC ACAGATGAAG
            1141 GTGCCCTCCT ATTGAATGCC ATTAGGG
            //
            Primer: M13_-40
            Sequence file: RDB13001_A4Jsb.seq check
            >06178_13001_A4Js_M13_-40_B03_06.ab1
                1 AAGGCGGTTA ATCGAGCTCG GGAGCGCTAC CGTGAATGGG CTCAACCAAA CAATGAACCT
               61 TTTCTTCTGA TTTTCCTACC ACACCGGAAA GGGAGGCAGA AGGCAAAAGG CCCAAGCTAC
              121 CAGGAATGAC AGAAGCCTCA TCTGAAATAA TGTCACCAAA CAAGTTGTCA GTCAAAACAA
              181 CACCGTTAAG TGTACGAGGG CTCTTGACCA AAAGCATGGC TGCGGAGTCA ATGAGCTGGT
              241 TTTTTAAGGT AAGGTGAGGA TATTCCTCCT TAAAAATCTT AGCTACAGTC TTGCGCCAAA
              301 GACGAGAAGT TGCCAAAACA TTAGCTTTGT CGAGTAATGT GACGGGAGCA GGAGGGTTGG
              361 AAGTTTCAGC TAAGCGGCCG CGGGCCCAAC CTTCCAATTC ATTAAATCGA GTCCTCCATC
              421 AGATGATTTA CAAGGTTTTG CATAGAATCC CTAAGTAAGA GAAGTTGTTA GTGATTTCTT
              481 CAAGTTTACT AAAATTTACA TACAAATGGA TGATCTCTTC TCCATTGTTT CCTTTCTTCT
              541 TGCAAACGCG TTTTACAAAG AGATGACATG CTATTTTCTG ATTATTTTTT TCTATAGTTT
              601 TCTATTTTGT ACGTCCTTGG GAGTCCGAAA TGTAAAATCG GGGTATGGTT AGTAGCGGCA
              661 GCGTTCATTA TGGTATATAA AAGATAAAAA GTAGGGGCAA GCGAGACAGA ATGGGATACA
              721 AGGGCATCGT CTATAATTAT AGCTAAAAAT TGTATTTTAA TTTGTATTTT TTGTAATTTT
              781 ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTAGAGA AAACTTCAAC GAAATGTCAA
              841 AATAAGCTCA GCAAAATATA CAATTTTAGG GAAAGCGATC AGCAACTTAT TCTCCGTTGT
              901 AGATTCTTTT TCTTCATAAA TTCCAACAAT GTAACTGCCA ATTGATTTTT CTCTAGATGC
              961 TAATAAATTG AAACTAATGA ATATAATAAC ACAGGTTTTG ATAAATTTGT AAACTCTTAC
             1021 TCAAAAAAAG AATGTTAAAA CTAATTTAAC CCTTTTTTAT AAAGGCTCAT GGCGTTTAAA
             1081 GAGGATAGTA AAAAATCTAC ATTTTTTTAA TTAATGTCAC ACTGTCCTGC GATATATTCT
            1141 AATAGTTTTT CGCTACTGCC TGTTTTTAGT AACTAATTTA ACCT
            //
            Primer: ars_R1
            Sequence file: RDB13001_A4Jsc.seq check
            >06178_13001_A4Js_ars_R1_C03_09.ab1
                1 AAGGGGTCTG TTAATACTAA TTACTTAAGT ACCTTTAACT AAACAAAATG CCTATATATA
               61 TATTAATTTA CAATGAGTGT CAGATAAGTC ACTATGTCCG AGTGGTTAAG GAGTTAGACT
              121 CGAATTCGCA AGCTTAGCTA CAAATCCCAC TGGCTATATG TATGCATTTG TGTTAAAAAA
              181 GTTTGTATAG ATTATTTAAT CTACTCAGCA TTCTTTCTCT AAATAGGAAT TTGTTACTTA
              241 ATGGAGAAAA AAATGTTTCG ATTTACCTAG TGTATTTGTT TGTATACTCA CGTTTAATTT
              301 CAAACATCCA TTCTATCTTG TGTAATTTTT GGCATGGTGA AAAAGATAAT CAGCCTTATA
              361 ATCTTTACAA AAGTAAGAAA TTCTGTAAAT AAGCCTTAAT GCCCTTGCTT TAAATTAAAA
              421 TGGTTCTTTT TCATGATAAT GTTTGCACTT TGTGAATATA TTTTAGATAG TTCTGTGAGG
              481 TATAATTAAG ATGTTTTAGA GACTTATACA ATTTTGTCTT TATAAATTCT TAATTGATTT
              541 TACCATCCCA GTTTAACTAT GCTTCGTCGG CATCTCTGCA CATGTCGTGT TTTCTTACCG
              601 TATTGTCCTA CCAAGAACCT CTTTTTTGCT TGGATCGAAA TTAAAGGTTT AAAAGCAAAG
              661 TTATGGATGC TAGAGTATTT CAAAGCTATT CAGCTAGAGC TGAGGGGATG AAAAATCCCA
              721 TTGCCAAGGA ATTGTTGGCT TTGATGGAAG AAAAGCAAAG CAACTTGTCA GTCGCGGTCG
              781 ATTTGACGAA GAAATCCGAA ATCTTAGAAT TGGTAGATAA AATTGGACCC TATGTCTGTG
              841 TTATCAAGAC ACATATTGAC GTTGTCGAGG ATTTCGACCA GGATATGGTA GAAAAACTGG
              901 TGGCCTTAGG TAAAAAGCAT CGTTTTCTTA TCTTTGAGGA TCGCAAATTC GCAGACATTG
              961 GAAATACCGT CAAGCTACAA TATGCATCTG GTGTGTACAA AATTGCTTCT TGGGCTCATA
             1021 TCACAAATTG CCATACAGTG CCCAGGCGAG GGTATTATAC AGTCCTCAAA AGAAGTTGGG
             1081 TTACCTTTGG GACGTTGATC TCTTGCTTTG GCCTGAAATG TCTCCAAAGG CTCTTTGCTA
            1141 CCTGATCTAC CCAGAGAAAA CCTTAGGAAA TGGAGTTTTG AGAAAGCCAA TATGCACG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913.
            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.
            reference Yu, H., Conserved Sequence Preferences Contribute to Substrate Recognition by the Proteasome. J. Biol. Chem. 291 (28): 14526-14539 (2016). PMID 27226608.
            reference Huang, S., Identification of Methylosome Components as Negative Regulators of Plant Immunity Using Chemical Genetics. Mol. Plant 9 (12): 1620-1633 (2016). PMID 27756575.
            user_report Gu, M., LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc. Natl. Acad. Sci. U.S.A. 114 (11): E2166-E2175 (2017). PMID 28242692.
            user_report Boehringer J., Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation. Biochem. J. 448 (1): 55-65 (2012). PMID 22906049.
            user_report Wen, J., Splicing-dependent NMD does not require the EJC in Schizosaccharomyces pombe. EMBO J. 29 (9): 1537-1551 (2010). PMID 20360683.
            user_report Snaith, H.A., New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol., 97: 147-172 (2010). PMID 20719270.

            2023.05.02

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