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pKM2L-phK5

Promoter Bank clone, Human keratin K5 promoter

Catalog number RDB05886
Resource name pKM2L-phK5
Alternative name p1931-27F
Clone info. PCR amplified human keratin K5 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 52526648 to 52520363(NC_000012.12); relatively -6189 to +97, where +1 corresponds to 1 nt of NM_000424.4.
Comment PCR cloning, forward primer, 1931 F-SpeI: 5' gactactagtgaattctaactccttattatcagcc 3'; reverse primer, 1931 R-SalI: 5' gactgtcgacgagctcagcaggacgcagaaggtgg 3' Entire sequence of promoter region has not been confirmed. (reference: Mol. Cell. Biol., 13, 3176-3190, 1993, PMID:7684490; Mol. Cell. Biol., 9, 3685-3697, 1989, PMID:2476664)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human keratin 5 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human keratin 5
          Reference sequence
            AC055736
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05886 pKM2L-phK5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phK5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05886).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Dnaconda's recommendation EXR0015j (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17218_A9Dbp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 ()
          Sequence file: RDB17218_A9Dba.seq check
          >05886_17218_A9Db_2_M13_-40_A01_01_ABI08.ab1
              1 AAGGCCAGGG CCAGGCTTGC ATGCCTGCAG GTCATTTAAA TAGGGATAAC AGGGTAATGA
             61 TTAAGATCTC GAGCTCTAAG CTTCACATAT GCATGCACTA GTGAATTCTA ACTCCTTATT
            121 ATCAGCCACT GGAGTAGAGG CGATCAGCGT CTGCAGACTG GTGGGACCTC ATGGCCTAGA
            181 GCAGACAAAT GCCTTGGAGG AACAGGCTTC GGTGCTAACT TAGGCTTGAA GGCCAGTCTG
            241 CTCCCTGGAA AGACCCAGAC CAGCACCTGG ACATGAGGGC TCTGGACAGG GGAGGGGTGG
            301 GGAGCAGTAA AAACACCCTC ACCCATCACA GTCACCTGTC AGCCCTGTCA TGCACACAGC
            361 CCTCGGGAAA TGGCTCTCTG AAGAGGACTG AGACGTGCAG CCTTGGACTG GGAGACAAGT
            421 AGGCTCTTGC TGAGGCCCAG ATGGGCAGTG CAGGGCAACA ACAGCGTGTG GGACAGGACC
            481 AGGACCCAGG CTTGGCTCTC ACCTCACCTT GCCAAGGGCT TGGCGTTAGG CTCCACCTCC
            541 CTGAACTCAG CCTGTTCCTC TATGGATGGA TCACTGGGCT GGTTTTTTTC TCTGAAACCA
            601 AGTCAGGCCA GCTTCCCTAA CCCGGCCCTT GCACAGTGAC CCGTCACTGT CAAGTGGTGT
            661 GGGCCACCAT CCTGGGTCTG GTAAGGAGAT CCTAGAGGAG ACCCAGCCCT GTTCGCAGTC
            721 CTGGAGTCTG AGGGAGGAAA TGGGGAAGGG TACAGGGTCA CACAACCAGA GTCCAGAAGT
            781 CCAGTGCATG GCATCTGCCT TGGCCAAGTC ACACATGTGG AACAGGACCT GATTCAATGA
            841 CTGGCATGAA TTGAAACACT CCTGCACGCT CTCTTGTCTC AGTTGCTTTT TGCACTGCGT
            901 CTCCCTTGGA AGAAGCCTTA ACCTGTTTTC ACAAACCCTC AACAGTGATC GTTGGTCCAA
            961 GTGGACAGTG AGCAGGTCAG TGTTCACATG TGCTCACGGA ACATGAAGAT GGGAGCTGCG
           1021 AGTGTGTCCT GGAGTCTGAG GGAGGAAATG GGGAAAGGAC ACAGGGTCAC AGTCTCACAT
           1081 AGACGTGGAC TGGGGCCAGC AAGGTGTTCC CAATGGCCTT GAGGACTGTG CAGAATGCCG
           1141 AGTGCATTCT GAGGGTGGAC CTGGGGAGAA GCACAGCTTC AAGTCTTCAT GCCTGTCCAT
          1201 TTAACGTTCC CTTATTCTTG CTG
          //
          Primer: phRLR2primer ()
          Sequence file: RDB17218_A9Dbb.seq check
          >05886_17218_A9Db_2_phRLR2primer_B01_02_ABI08.ab1
              1 ATTTTCCCTT GTCTCGACAG CCCAGTTTCT ATTGGTCTCC TTAAACCTGT CTTGTAACCT
             61 TGATACTTAC CTGCCCAGTG CCTCACGACC AACTTCTGCA GTACCCGGGT GAATTCTACG
            121 CGTCGACGAG CTCAGCAGGA CGCAGAAGGT GGCTCTGTTA CCCAGGAACG GTGATGCCCC
            181 CTTTTTATCC CTTCCAGAAC TGGGCTGGGC GAGAGAGCTG TCGAGCTGTG AATGATTAAG
            241 TGGGCTGGGC ATGCCTGGGG GGCAGCTGTG AGCTCACCGG TCACACCTGC ACAGTGGTGT
            301 GGGGTGCAAA CGCTTTCTTC CTGGCAGGCT CTGCTCAGTT AGAAATAACC TTGCCTTGCA
            361 GCACTGATCT CTTGGCAGCA AGTTGGTGAC GCTGCTAGCA AGTATGTCTT CACCATAGAG
            421 GCTGGGGGCT GTGAGCAGCC CAAAAAGGAT GCCAGGAACT GAGGGGGCAT TGGCATGGCC
            481 AGTGTCCTGG GGACTCTTAC AGGACCAGGA GTATTGACAA CCATCCCAGT ACGGTACATG
            541 CTGAGCACCT GCCCTTCCAG CTTGGGGAAG CTGTCCATCA GCGGGGGGCA AACGATTCCC
            601 CAGTGATCTG CTCTTACACT TTTCCAGGCA GCAGGTTGCT GCAGGGTTTT CCCCTCTGGG
            661 AAAGGCAATC TGTAGCCTTA TATTCTTGGT TATGTGCTAG AGGGAAAATA GAGTTTCATC
            721 CTTTTCTGCA CAATTTTTTT CTTAGGGAAG GCACAGCTAT CCCAGAGCCC ACTGGGCAGA
            781 TCTGCCTGCA AGGTTTAGGC CAGAGAATGT GGGAAGCCCT CAGCGCCCCC TATAAACCAC
            841 TTTGACCTCT TTAAGGCCAG GTTTATCCTT CCATAGCTCC TCCTAACTCA GCATTTTTTC
            901 TGCCCTTTGA CCTTTTCCTT GCTCTTTGGC CTGGGGCAGG ACTCTGTGGG GGGTTTAGGA
            961 AACCCGGGGA TCCCATGCAA TCCCTTAAAA AAAAACCCTG ACACTCATGG GCTATAGCGT
           1021 CTCTCTTTGG GGGTAGCTAC TCTGCCGGGT TCCCCAGCCC CCCAGGTGTG TTCCAGCAAG
           1081 CTCTATTCCA CTAGGAAGAA ATGAGTCATC GTATGTGTTG ATACAGCTGA GCCGCCCACA
           1141 AAACAACATA CTGCAGAGCT TCTATCTCAT CAGACCATGT GAGAGTCTGG ACGGTCTTGT
          1201 TATAACCGGC GGAAACACAC ACCCTGGT
          //
          Primer: pAxCALNLF1 ()
          Sequence file: RDB17218_A9Dbc.seq check
          >05886_17218_A9Db_2_pAxCALNLF1_C01_03_ABI08.ab1
              1 CAACCAAATG AATCTTATCC ATGTCTGCTC GAAGCGGCCG CTCTAGAATT ACTGCTCGTT
             61 CTTCAGCACG CGCTCCACGA AGCTCTTGAT GTACTTACCC ATTTCATCTG GAGCGTCCTC
            121 CTGGCTGAAG TGGAGGCCCT TCACCTTCAC GAACTCGGTG TTAGGGAACT TCTTAGCTCC
            181 CTCGACAATA GCGTTGGAAA AGAACCCAGG GTCGGACTCG ATGAACATCT TAGGCAGATC
            241 GTCGCTGGCC CGAAGGTAGG CGTTGTAGTT GCGGACAATC TGGACGACGT CGGGCTTGCC
            301 TCCCTTAACG AGAGGGATCT CGCGAGGCCA GGAGAGGGTA GGCCGTCTAA CCTCGCCCTT
            361 CTCCTTGAAT GGCTCCAGGT AGGCAGCGAA CTCCTCAGGC TCCAGTTTCC GCATGATCTT
            421 GCTTGGGAGC ATGGTCTCGA CGAAGAAGTT ATTCTCAAGC ACCATTTTCT CGCCCTCTTC
            481 GCTCTTGATC AGGGCGATAT CCTCCTCGAT GTCAGGCCAC TCGTCCCAGG ACTCGATCAC
            541 GTCCACGACA CTCTCAGCAT GGACGATGGC CTTGATCTTG TCTTGGTGCT CGTAGGAGTA
            601 GTGAAAGGCC AGACAAGCCC CCCAGTCGTG GCCCACAAAG ATGATTTTCT TTGGAAGGTT
            661 CAGCAGCTCG AACCAAGCGG TGAGGTACTT GTAGTGATCC AGGAGGCGAT ATGAGCCATT
            721 CCCGCTCTTG CCGGACTTAC CCATTCCGAT CAGATCAGGG ATGATGCATC TAGCCACGGG
            781 CTCGATGTGA GGCACGACGT GCCTCCACAG GTAGCTGGAG GCAGCGTTAC CATGCAGAAA
            841 AATCACGGCG TTCTCGGCGT GCTTCTCGGA ATCATAGTAG TTGATGAAGG AGTCCAGCAC
            901 GTTCATTTGC TTGCAGCGAG CCCACCACTG AGGCCCAGTG ATCATGCGTT TGCGTTGCTC
            961 GGGGTCGTAC ACCTTGGAAG CCATGGTGGC TAGCCTATAG TGAGTCGTAT TAAGTACTCT
           1021 AGCCTTAAGA GCTGTAATTG AACTGGAGTG GACACTGTGG AGAGAAAGGC AAAGGGATGT
           1081 CAGTAAGACC ATAGGTGCCT ATCAGAAACG GCAAGAGTCT CTCTGTCTCG ACAAGCCCAG
           1141 TTTCTTATAT CCTCTTAACC TTGTCTTGGT AACTTGATAC TAACCTGGCC AAGTTGCCTT
          1201 CACGGAACAA ACTTCTGTGC AGATTTACCT CCG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Tanaka, S., Functional conservation of Gsdma cluster genes specifically duplicated in the mouse genome. G3 (Bethesda), 3 (10): 1843-1850 (2013). PMID 23979942.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl