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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phPGHS2(1)

Promoter Bank clone, Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter

Catalog number RDB05480
Resource name pKM2L-phPGHS2(1)
Alternative name p1888-4F
Clone info. PCR amplified human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Coned fragment: 186,682,006 to 186,680,342 (NC_000001.11), relatively -1579 to +86, where +1 corresponds to 1 nt of NM_000963.3.
Comment PCR cloning, forward primer, 1888 F3: 5' gaggaggagaaaggcttcctagatg 3'; reverse primer, 1888 R3: 5' gtctggctgtggagctgaaggag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human prostaglandin synthase-2/cyclooxygenase (PGHS-2/COX-2)

          Reference sequence
            AY229989, AY382629
           

          Distribution information

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          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          RDB05480 pKM2L-phPGHS2(1) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phPGHS2(1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05480).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05480_07Dkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBAC_lac_F (Pr0544)
          Region: insert 5'
          Sequence file: RDB05480_07Dka.seq check
          >05480_05480_B1HbF_pBAC_lac_F_A01_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNCTN GCNTGCCTGC AGGTCATTTA AATAGGGANA ACAGGGTAAT
             61 GATTAAGATC TCNNGCTCTA AGCTTCACAT ATGCATGCAC TAGTGGCGAG GAGGAGAAAG
            121 GCTTCCTAGA TGAGATGGAA TTTTAGTCAT CCGTGTCTCA TGAAGAATCA GATGTGTACA
            181 CTAAGCAAAA CAGTTAAAAA AAAAACCTCC AAGTGAGTCT CTTATTTATT TTTTTCTTAT
            241 AAGACTTCTA CAAATTGAGG TACCTGGTGT AGTTTTATTT CAGGTTTTAT GCTGTCATTT
            301 TCCTGTAATG CTAAGGACTT AGGACATAAC TGAATTTTCT ATTTTCCACT TCTTTTCTGG
            361 TGTGTGTGTA TATATATATG TATATATACA CACACACATA TACATATATA TATTTTTTAG
            421 TATCTCACCC TCACATGCTC CTCCCTGAGC ACTACCCATG ATAGATGTTA AACAAAAGCA
            481 AAGATGAAAT TCCAACTGTC AAAATCTCCC TTCCATCTAA TTAATTCCTC ATCCAACTAT
            541 GTTCCAAAAC GAGAATAGAA AATTAGCCCC AATAAGCCCA GGCAACTGAA AAGTAAATGC
            601 TATGTTGTAC TTTGATCCAT GGTCACAACT CATAATCTTG GAAAAGTGGA CAGAANGACA
            661 AAAGAGTGAA CTTTAAAACT CGNNTTTATT TTACNNGTAT CTCCTATNNN NGGCTANNAN
          721 CCAAANNAAN NNNNNNNNN
          //
          Primer: phRLR2primer (Pr0083)
          Region: chimeric intron,insert 3'
          Sequence file: RDB05480_07Dkb.seq check
          >05480_05480_B1HbF_phRLR2primer_B01_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NTTTCNATTG GTCTCCTTAA ACCTGTCTTG TAACCTTGAT
             61 ACTTACCTGC CCAGTGCCTC ACGACCAACT TCTGCAGTAC CCGGGTGAAT TCTACGCGTC
            121 GACAGGCGTC TGGCTGTGGA GCTGAAGGAG GCGCTGCTGA GGAGTTCCTG GACGTGCTCC
            181 TGACGCTCAC TGCAAGTCGT ATGACAATTG GTCGCTAACC GAGAGAACCT TCCTTTTTAT
            241 AAGACTGAAA ACCAAGCCCA TGTGACGAAA TGACTGTTTC TTTCCGCCTT TTCGTACCCC
            301 CCACAAATTT TTCCCTCCTC TCCCCTTAAA AAAATTGCGT AAGCCCGGTG GGGGCAGGGT
            361 TTTTTACCCA CGGAAATGAG AAAATCGGAA ACCCAGGAAG CTGCCCCAAT TTGGGAGCAG
            421 AGGGGGTAGT CCCCACTCTC CTGTCTGATC CCTCCCTCTC CTCCCCGAGT TCCACCGCCC
            481 CAGGCGCACA GGTTTCCGCC AGATGTCTTT TCTTCTTCGC AGTCNNNNNN NGCGCTNNCN
          541 ANAGNCAGNT CTNNNNTGNN CGNNNNNNNG NNNANNNGNN NNGNNANNNN NNNNNN
          //
          Primer: SV40pA_R (Pr0882)
          Region: SV40 pA,hRluc 3'
          Sequence file: RDB05480_07Dkc.seq check
          >05480_05480_B1HbF_SV40pA_R_C01_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNTTTNN NAAATAAAGC ATTTTTTTNN CTGCATTCTA GTTGTGGTTT
             61 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGCTCGAAG CGGCCGCTCT AGAATTACTG
            121 CTCGTTCTTC AGCACGCGCT CCACGAAGCT CTTGATGTAC TTACCCATTT CATCTGGAGC
            181 GTCCTCCTGG CTGAAGTGGA GGCCCTTCAC CTTCACGAAC TCGGTGTTAG GGAACTTCTT
            241 AGCTCCCTCG ACAATAGCGT TGGAAAAGAA CCCAGGGTCG GACTCGATGA ACATCTTAGG
            301 CAGATCGTCG CTGGCCCGAA GGTAGGCGTT GTAGTTGCGG ACAATCTGGA CGACGTCGGG
            361 CTTGCCTCCC TTAACGAGAG GGATCTCGCG AGGCCAGGAG AGGGTAGGCC GTCTAACCTC
            421 GCCCTTCTCC TTGAATGGCT CCAGGTAGGC AGCGAACTCC TCAGGCTCCA GTTTCCGCAT
            481 GATCTTGCTT GGGAGCATGG TCTCGACGAA GAAGTTATTC TCAAGCACCA TTTTCTCGCC
            541 CTCTTCGCTC TTGATCAGGG CGATATCCTC CTCGATGTCA GGCCACTCGT CCCAGGACTC
            601 GATCACGTCC ACGACACTCT CAGCATGGAC GANGGNCTTG ANCTTGTCTT GNTGCTCGTA
            661 NGANTAGTGA AAGGNNAGAN NAGCCCCCCN NNCGNNNNNN NCAANGNNGN NTTTCTTNNN
          721 NANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Afroz, S., Dengue Virus Induced COX-2 Signaling Is Regulated Through Nutrient Sensor GCN2. Front. Immunol. 11: 1831 (2020). PMID 32903536.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl