RDB04391 - CS-RfA-EG

Resource data sheet
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CS-RfA-EG

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.

Catalog number	RDB04391
Resource name	CS-RfA-EG
Clone info.	Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with GFP marker driven by EF-1 alpha promoter.
Comment	Commonly requested with pENTR4-H1 (RDB04395) for generation of shRNA expression vector and with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394) for packaging.
Vector backbone	Plasmid
Selectable markers	Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

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External Database

Reference sequence

Sequence	RDB04391z.seq
Remarks, protocol and/or map (pdf)	RDB04391.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Ordering
Order form [Credit Card Payment] [Bank Transfer Payment]
MTA, for use for not-for-profit academic purpose)[Word]
Please visit Ordering instruction.[link]

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04391	CS-RfA-EG	DNA solution

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-RfA-EG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04391).

Reference section:

Miyoshi, H., Blömer, U., Takahashi, M., Gage, F.H., Verma, I.M., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Lentiviral plasmid list (English text)
Featured content	Lentivirus Vector (English text)
Featured content	Lentivirus Vector (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18667_B1Afp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: RfA(CmR,attR1),cTTP_CTS Sequence file: RDB18667_B1Afg.seq
>04391_18667_B1Af_1_Cm_M_R_B01_04_ABI24.ab1 1 NNNNNNNNNN NNTCNNNNAT NNCCNGCTCN CCGTCTTTCN TTGCCNTACG GAATTCCGGA 61 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT 121 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT 181 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG 241 GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAC 301 GGATCCTAAC TCAAAATCCA CACATTATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 361 GTGCCTAATG CGGCCGCCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT 421 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT 481 CAGCTTTTTT GTACAAACTT GTGATAATTC AAAGTGAATT TCTGCTGTCC CTGTAATAAA 541 CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT ATGTCTGNTG 601 CTATTATGTC TACTATTCNT TCCCCTGCAC TGTNNCCCCN NATCCCCCCT TTTCNTTTAA 661 AATTGNGNAN GAANANNGCC NTTTGNANAT NCNNTCACNN ANCNAANNNN NCNNTCTCTG 721 NNNNNCTNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,EGFP 3' Sequence file: RDB18667_B1Afh.seq
>04391_18667_B1Af_1_PRE_rev_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGG ANCANCATAG TTAAGAATAC CAGTCAATCT TTCACAAATT 61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GAGATCCTAC TAGTAGGATC 121 TGAGTCCGGA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT 181 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC 241 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT 301 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT 361 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT 421 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC 481 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT 541 CCTTGAAGAA GANGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT 601 CGTGCTGCTT CNTGTGGTCN GGGTAGCGGC TGAAGCACTG CNCGCCNTNN NCNNNGNTGG 661 TCNCNANGGT GGGCCANGGN ACGGGCAGNT TGNNNGNGNN GNNNANGANN NTNNNGNNNN 721 NNTTGNCNNN NNNNGNNNNN NNNCNNNNNC NNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,RfA(attR2,ccdB) Sequence file: RDB18667_B1Afi.seq
>04391_18667_B1Af_1_EF1a-N_R_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNGGCG ANGTGCGCTC TGCCNNCTGA CGGGCACCGG ANCCTCACGA 61 AGCTTGAATT ATCACCACTT TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA 121 TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA 181 ACATATCCAG TCACTATGGT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT 241 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT 301 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC 361 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA 421 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC 481 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT 541 GCATTTCACC AGCCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG 601 ACCTCAGCCA TCCCTTCCTG ANTTTCCGCT TTCCAGCGTT CGGCACGCAG ANGANGGGCT 661 TCATTCTGCN TGGTTGNGCT TANNNNANCN GNANANANTG ACNTCATATA TGCNNNGAGC 721 AACTGANANC NGTCNNNNNN //
Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,EGFP 5' Sequence file: RDB18667_B1Afj.seq
>04391_18667_B1Af_1_EF1a-C_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNTTTNANT TTGGNNCTTG GTTNNTTCTC NAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AACACGCTAC CGGTCGCCAC CATGGTGAGC 121 AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA 181 AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG 241 ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC 301 ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC 361 TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC 421 GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC 481 ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG 541 TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG 601 GTGAACTTCA AGATCCGCCA CAACATCGAG GANGGCAGCG TGCANCTCGC CNANCACTAN 661 NNGCAGAANN NCCCNNTCGG NNANNNCCCC NNGCNGCTNN CCNANNNNNN NNACCNGANN 721 NCCNNNNNNN NCNNNNNNNA NNNNCCNNNN NNNNNN //
Primer: CMV_Forward (Pr0016) Region: 5' LTR,HIV-1 psi Sequence file: RDB18667_B1Afk.seq
>04391_18667_B1Af_1_CMV_Forward_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNTG NNNCNNNGGG CGGTAGGCGT GTACNGNGGG AGGTCTATAT 61 AANCAGCGCG TNTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA 301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA 361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA 421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG 481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA 541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT 601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA 661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC 721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGANC ACCGCACAGC AAGCGGCCGC 781 TGATCTTCAG ACCTGGAGGA NGAGATATGA NGGACAATTG GAGAAGTGAA TTATATAAAT 841 ATAAAGTAGT AAAAATTGAA CCATTANGAG TAGCACCCAC CAAGGCAAAG ANAAGAGTGG 901 TGCAGANAGA AAAAAGAGCA GNGGGAATAN GANCTTTGTT CCTNNGNNNC NNGGGNNCNN 961 CNNNNANNCA CTATGGNNNC ANCGNNNNNG NNNCNGNNNN NNCNNNNCNN NNNNNNNN //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi Sequence file: RDB18667_B1Afl.seq
>04391_18667_B1Af_1_HIVgp_R1_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNCTCT TTTTTCTCTC TGCACCNCTC TTCTCTTTGC CTTGGTGGGT 61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG 121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GCTTGCTGTG CGGTGGTCTT 181 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT 241 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA 301 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC 361 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT 421 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT 481 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT 541 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC 601 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCNCTTTCN AGTCCCTGTT 661 CNGGCGNNNC NGCTANAGAN TTTNCNCACT GANTAAANNN NCTGNNGNNN CTCTANNNAN 721 NNNANTCNCN CNNNNNANNG NNNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3' LTR Sequence file: RDB18667_B1Afm.seq
>04391_18667_B1Af_1_bGH_rev3_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNGGNNGNN GGGCNNACNA CAGATGGCTG GCAACTAGAA GGCACAGTCG 61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT 121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT 181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG 241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG 301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC 361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT 421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG 481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC 541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCNG TCCCCCCTTT 601 TCTTTTAAAA AGTGGCTAAN ATCTACAGCT GCCTTGTAAG TCNTTGGTCT TAAAGGNANC 661 TGANGTGTGA NTGNAAAANC NNNCTNCTCC TNCTCTNGNG CNTNTANNNN NGCNCNNNCN 721 NNANTNGNNA NCTGCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB18667_B1Afn.seq
>04391_18667_B1Af_1_SV40pro_ori_F_G01_19_ABI24.ab1 1 NNNNNNGNNN NNGCCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT 61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG 181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG 241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG 301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC 361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG 421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG 481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG 541 TGCACTTCGT GGCCGAGGAG CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG 601 CCTTCTATGA AAGGNTGGGC TTCGGANTCG TTTTCCGGGA NGCCGGCTGG ANGATCCTCC 661 NGCGCGGGGN NCTCNTGCTG GANTTCTTCG CCCACCCNNN NNNGNTTATN GNNNCTTNNN 721 NNGGNNNNNA NTAANNCNNN NNNNNNN //

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References

Original, user report and related articles

original	Miyoshi, H., Development of a self-inactivating lentivirus vector. J. Virol., 72 (10): 8150-8157 (1998). PMID 9733856. [link to RRC of NBRP]
user_report	Moriuchi, T., SUMOylation of RepoMan during late telophase regulates dephosphorylation of lamin A. J. Cell Sci. 134 (17): jcs247171 (2021). PMID 34387316. [link to RRC of NBRP]
user_report	Kawasaki, N., TUFT1 interacts with RABGAP1 and regulates mTORC1 signaling Cell Discovery 4 (1): (2018). PMID 29423269. [link to RRC of NBRP]
user_report	Yoshitomi, H., Human Sox4 facilitates the development of CXCL13-producing helper T cells in inflammatory environments. Nat. Commun. 9 (1): 3762 (2018). PMID 30232328. [link to RRC of NBRP]
user_report	Ishibashi, M., CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors. Sci. Rep. 7: 46662 (2017). PMID 28429795. [link to RRC of NBRP]
user_report	Kobayashi, S., TGF-beta induces the differentiation of human CXCL13-producing CD4(+) T cells. Eur. J. Immunol. 46 (2): 360-371 (2016). PMID 26541894. [link to RRC of NBRP]
user_report	Katsura, A., MicroRNA-31 is a positive modulator of endothelial-mesenchymal transition and associated secretory phenotype induced by TGF-beta. Genes Cells 21 (1): 99-116 PMID 26663584. [link to RRC of NBRP]
user_report	Takahashi, Y., Perilipin2 plays a positive role in adipocytes during lipolysis by escaping proteasomal degradation. Sci Rep. 6: 20975 (2016). PMID 26876687. [link to RRC of NBRP]
user_report	Inoue, J., Identification of BCL11B as a regulator of adipogenesis. Sci. Rep. 6: 32750 (2016). PMID 27586877. [link to RRC of NBRP]
user_report	Neri, S., Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma. Int. J. Cancer. 137 (4): 784-796 (2015). PMID 25648219. [link to RRC of NBRP]
user_report	Tanaka, Y., Dual Function of Histone H3 Lysine 36 Methyltransferase ASH1 in Regulation of Hox Gene Expression. PLoS One., 6 (11): e28171 (2011). PMID 22140534. [link to RRC of NBRP]
user_report	Ohoka, N., The orphan nuclear receptor RORalpha restrains adipocyte differentiation through a reduction of C/EBPbeta activity and perilipin gene expression. Mol. Endocrinol., 23, 759-771 (2009). PMID 19324970. [link to RRC of NBRP]
user_report	Naka, H., Requirement for COUP-TFI and II in the temporal specification of neural stem cells in CNS development. Nat. Neurosci. 11 (9): 1014-1023 (2008). PMID 19160499. [link to RRC of NBRP]
user_report	Fumoto, T., Orphan nuclear receptor Nur77 accelerates the initial phase of adipocyte differentiation in 3T3-L1 cells by promoting mitotic clonal expansion. J. Biochem. 141 (2): 181-192 (2007). PMID 17167038. [link to RRC of NBRP]
user_report	Tsukamoto H., TCR ligand avidity determines the mode of B-Raf/Raf-1/ERK activation leading to the activation of human CD4+ T cell clone. Eur. J. Immunol., 36, 1926-1937 (2006). PMID 16791876. [link to RRC of NBRP]

2024.04.16

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