Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-CMV-RfA-IRES2-Venus

Backbone vector plasmid of lentivirus construct driven by CMV promoter by Gateway(R) cloning with Venus fluorescent protein marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08941_A5Kip1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CSII-CMV5' (Pr0021) Region: attR1,CmR Sequence file: RDB08941_A5Kia.seq
>04388_08941_A5Ki_CSII-CMV5'_A05_02.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC TGAACGAGAA ANNGTAAAAT GATATAAATA    61 TCAATATATT AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC   121 ATATCCAGTC ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG   181 CTCGTATAAT GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC   241 TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA   301 AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT   361 GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT   421 TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA   481 CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC   541 TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT   601 ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT   661 TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA   721 CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA   781 AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT   841 CCATGTCNGC AGAATGCTTA ATGAATTACN ACAGTACTGC NATGANTGGC ANGGCGGGGC   901 GTAAACGCGN GNATNCNGCT NACTAAAAGC CAGATAACAG TATGCGNATT TGCNCNCTNA   961 NTTTTGCNGN ANNNNANNNT ATACNGNTAT GTATANCNNA NNNATGTNAA AAANNNNNNN 1021 NNN //
Primer: IRES-R (Pr0037) Region: attR2,ccdB Sequence file: RDB08941_A5Kib.seq
>04388_08941_A5Ki_IRES-R_B05_05.ab1     1 NNNNNNNNNN NNNNNNNNNA NGTNAGGGGG GGGGGNNNNN NANGGGCGGN TCCGCGGCCG    61 CTGCAGAATT CTCGAGACCG GTAGCATCAC CACTTTGTAC AAGAAAGCTG AACGAGAAAC   121 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA   181 ATACTGTAAA ACACAACATA TCCAGTCACT ATGGTCGACC TGCAGACTGG CTGTGTATAA   241 GGGAGCCTGA CATTTATATT CCCCAGAACA TCAGGTTAAT GGCGTTTTTG ATGTCATTTT   301 CGCGGTGGCT GAGATCAGCC ACTTCTTCCC CGATAACGGA GACCGGCACA CTGGCCATAT   361 CGGTGGTCAT CATGCGCCAG CTTTCATCCC CGATATGCAC CACCGGGTAA AGTTCACGGG   421 AGACTTTATC TGACAGCAGA CGTGCACTGG CCAGGGGGAT CACCATCCGT CGCCCGGGCG   481 TGTCAATAAT ATCACTCTGT ACATCCACAA ACAGACGATA ACGGCTCTCT CTTTTATAGG   541 TGTAAACCTT AAACTGCATT TCACCAGCCC CTGTTCTCGT CAGCAAAAGA GCCGTTCATT   601 TCAATAAACC GGGCGACCTC AGCCATCCCT TCCTGATTTT CCGCTTTCCA GCGTTCGGCA   661 CGCAGACGAC GGGCTTCATT CTGCATGGTT GTGCTTACCN GACCGGANAN ATTGACATCA   721 TATATGCCTT GAGCNACTGA TAGCTGTCGC TGTCNACTGT CACTGTAATA CGCTGCTTCA   781 TANCATACCT CTTTTTGACA TACTTCGGNT ATACATATCA GTATATATTC NTATACNGCA   841 AAAATCAGCG CGCAAATACN CNTACTGNTA TCTGGNTTTT ANTAANCCNG ANNCNNNCNN   901 TTACGCCCCG NCCNGNNNNN CNTCNNANNA NTGNNGNNAN TNNNTNNNNN ATNCNGNNNN 961 NNTNNNNNNN ATCNNNAANN NNNNNNNGNN NNNNNNNN //
Primer: IRES-F (Pr0036) Region: IRES2,Venus,PRE Sequence file: RDB08941_A5Kic.seq
>04388_08941_A5Ki_IRES-F_C05_08.ab1     1 NNNNNNNNNN NNNNNNNNNN TCNANGTTNA NNANNCGTCT AGGCCCCCCG AACCNCGGGG    61 ANGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGCTGATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 GGCTACGGCC TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ANCGCCGCCG GGATCACTCT CGGCATGGNC GANCTGTACN AGTAAGAATT CAAGGCCTCT   841 CGAGCCTCTA NAGTTAACAT CGAGGGNNCA AGCTTATCGA TAATCAACCT CTGGANTACA   901 AAATTTGTGA AAGANTGNNT GGTANNCTTA ACTATGTTGC TCCTTTTANN CTATGTGNAN   961 ACGCNGCTTT AANGNCTTTG TNTCANGNNA NTNGNTTCCC GNANGNNNTN CATTTTNNNN 1021 NNCNTNNNNN NNNNN //
Primer: HIVgp_R1 () Sequence file: RDB08941_A3Dmd.seq
>04388_08941_A3Dm_HIVgp_R1_D11_11.ab1     1 CAGCTCTATT CCACTGCTCT TTTTTCTCTC TGCACCACTC TTCTCTTTGC CTTGGTGGGT    61 GCTACTCCTA ATGGTTCAAT TTTTACTACT TTATATTTAT ATAATTCACT TCTCCAATTG   121 TCCCTCATAT CTCCTCCTCC AGGTCTGAAG ATCAGCGGCC GGCCGCTTGC TGTGCGGTGG   181 TCTTACTTTT GTTTTGCTCT TCCTCTATCT TGTCTAAAGC TTCCTTGGTG TCTTTTATCT   241 CTATCCTTTG ATGCACACAA TAGAGGGTTG CTACTGTATT ATATAATGAT CTAAGTTCTT   301 CTGATCCTGT CTGAAGGGAT GGTTGTAGCT GTCCCAGTAT TTGTCTACAG CCTTCTGATG   361 TTTCTAACAG GCCAGGATTA ACTGCGAATC GTTCTAGCTC CCTGCTTGCC CATACTATAT   421 GTTTTAATTT ATATTTTTTC TTTCCCCCTG GCCTTAACCG AATTTTTTCC CATCGCGATC   481 TAATTCTCCC CCGCTTAATA CTGACGCTCT CGCACCCATC TCTCTCCTTC TAGCCTCCGC   541 TAGTCAAAAT TTTTGGCGTA CTCACCAGTC GCCGCCCCTC GCCTCTTGCC GTGCGCGCTT   601 CAGCAAGCCG AGTCCTGCGT CGAGAGAGCT CCTCTGGTTT CCCTTTCGCT TTCAAGTCCC   661 TGTTCGGGCG CCACTGCTAG AGATTTTCCA CACTGACTAA AAGGGTCTGA GGGATCTCTA   721 GTTACCAGAG TCACACAACA GACGGGCACA CACTACTTGA AGCACTCAAG GCAAGCTTTA   781 TTGAGGCTTA AGCAGTGGGT TCCCTAGTTA GCCAGAGAGC TCCCAGGCTC AGATCTGGTC   841 TAACCAGAGA GACCCAGTAC AGGCAAAACG CGCTGCTTAT ATAGACCTCC CACCGTACAC   901 GCCTACCGCC CATTTGCGTC AATGGGGGCG GAGTTGTTAC GACATTTTGG AAAGTCCCGT   961 TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGAAATCC  1021 CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CCAAAACCGC ATCACCATGG  1081 TAATAGCGAT GACTAAATAC GTAGATGTAC TGGCTAGGTT AGAAAGGTCC CATATGTTCA 1141 TGTACTGGGG CATAATCCCA AGGGCCGGGG CCCAATA //
Primer: BGH_rev () Sequence file: RDB08941_A3Dme.seq
>04388_08941_A3Dm_BGHrev_E11_14.ab1     1 GGTACGGGCT GCTAGAGATT TTCCACACTG ACTAAAAGGG TCTGAGGGAT CTCTAGTTAC    61 CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC TCAAGGCAAG CTTTATTGAG   121 GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA GGCTCAGATC TGGTCTAACC   181 AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT GTGATGAAAT GCTAGGCGGC   241 TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC CATCCCATGC AGGCTCACAG   301 GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC TACCTTCTCT TGCTCAACTG   361 GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT GATCCCTGGC CCTGGTGTGT   421 AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT CCACAGATCA AGGATATCTT   481 GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT TTCTTTTAAA AAGTGGCTAA   541 GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC CTGAGGTGTG ACTGGAAAAC   601 CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC AGCATTGGTA GCTGCTGTAT   661 TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG GTATCGATGC GGGGAGGCGG   721 CCCAAAGGGA GATCCGACTC GTCTGAGGGC GAAGGCGAAG ACGCGGAAGA GGCCGCAGAG   781 CCGGCAGCAG GCCGCGGGAA GGAAGGTCCG CTGGATTGAG GGCCGAAGGG ACGTAGCAGA   841 AGGACGTCCC GCGCAGAATC CAGGTGGCAA CACAGGCGAG CAGCCATGGA AAGGACGTCA   901 GCTTCCCCGA CAACACCACG GAATTGTCAG TGCCCAACAG CCGAGCCCCT GTCCAGCAGC   961 GGGCAAAGGC AGGCGGCGAT GAGTTCCGCC GTGGCAATAG GGAGGGGGAA AGCGAAAGTC  1021 CCGGGAAAGG AGCTGACAGG TGGTGGCAAT GCCCCAACCA GTGGGGGGTT TGCGTCAGCA  1081 AACACAGTGC ACACCACGCC ACGTTTGCCT GACACGACAC ACTCCTCATT AAAGAGACAG 1141 CACCAAGGAT TTTTATTACC AACGGAAGGG AGAAAAAATT GA //
Primer: SV40pro_F () Sequence file: RDB08941_A3Dmf.seq
>04388_08941_A3Dm_SV40pro_F_F11_17.ab1     1 GCCTGTCCTG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA    61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT   121 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA   181 AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT   241 GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC   301 GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC   361 TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT   421 CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG   481 GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC   541 AGGACTGACA CGTGCTACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT   601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG   661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA   721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA   781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT   841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA   901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT   961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCCTGTCGTG CCAGCTGCAT  1021 TAATGAATCG GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGGCGC TCTTCGCTTT  1081 CTCGCCTCAC TGACTCGCTG CCGCTCCGCT CGATCCGGCT GCCGCGAAGC GTAATCCAGC 1141 TCACTCAAGC GTATACGGTT AATTCCACCA G //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB08941_A5Kig.seq
>04388_08941_B0G9S_SV40pro_ori_F_E10_13_ABI24.ab1     1 NNNNNNNNNN NNNNNGNCGC CTCTGCCTCT GNNCTATTCN NGAAGTAGTG AGGANGCTTT    61 TTTGGAGNCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT   121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA   181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG   241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG   301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG   361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT   421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA   481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT   541 GCGTGCACTT CGTGGCCGAN GANCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG   601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC   661 TCCAGCGCGG GGNTCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT   721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTTCA   781 CTGCATTCTA GTTGTGGNTN GNCNNAACTC ATCAATGTAT CTTATCATGT CTGTATACNN   841 NCNANNNCNA NCNANNNCTT GNCNNNATCN NNGNNCATAN CNGTTTCCNN NNNNAAANNN 901 NNNTNCGNNN ANNNNNNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl