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CSII-CMV-MCS

Backbone vector plasmid of lentivirus construct with CMV promoter.

Catalog number RDB04377
Resource name CSII-CMV-MCS
Alternative name pCSII-CMV-MCS
Clone info. Backbone vector plasmid of lentivirus construct with CMV promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone Plasmid
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence RDB04377z.seq
            Remarks, protocol and/or map (pdf) RDB04377.pdf
            Lentiviral plasmid list. [link]

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB04377 CSII-CMV-MCS DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSII-CMV-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04377).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17907_B0Aep1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: cPPT+CTS_F (Pr0161)
            Region: CMV en2, CMV pro2, MCS, PRE
            Sequence file: RDB17907_B0Aea.seq check
            >04377_17907_B0Ae_1_cPPT+CTS_F_A07_01_ABI24.ab1
                1 NNNNNNNNNN NNNNTCGNNT AATAGTAANN NATTACGGGG TCATTAGTTC NTAGCCCATA
               61 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA
              121 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT
              181 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT
              241 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA
              301 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
              361 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT
              421 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA
              481 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG
              541 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT
              601 CCGCTAGCGC TACCGGTCTC GAGAATTCTG CAGCGGCCGC GGATCCTCTA GAGTTAACAT
              661 CGAGGGATCA AGCTTATCGA TAATCAACCT CTGGANTACA AAATTTGTGA AAGANTGNCT
              721 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
              781 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG
              841 CTGTCTCNTT ATGANGAGTT GTGGCCCNTT NNCANGNAAC GNNNCNNNNN NNGCNNNNNN
            901 NTNGCNNANN CNACCCCCAC NNGNNNGGGG NNTTGCCNNN NNCNNNNNNN NNN
            //
            Primer: HIV-B_R (Pr0673)
            Region: HIV-1 psi, 5'LTR, CMV pro1, CMV en1
            Sequence file: RDB17907_B0Aeb.seq check
            >04377_17907_B0Ae_1_HIV-B_R_C07_07_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NTTTTGGCGT ANTCNCCNGT CGCCGCCCCT CGCCTCTTGC
               61 CGTGCGCGCT TCAGCAAGCC GAGTCCTGCG TCGAGAGAGC TCCTCTGGTT TCCCTTTCGC
              121 TTTCAAGTCC CTGTTCGGGC GCCACTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG
              181 AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA
              241 GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT
              301 CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGGCAAAAC GCGCTGCTTA TATAGACCTC
              361 CCACCGTACA CGCCTACCGC CCATTTGCGT CAATGGGGCG GAGTTGTTAC GACATTTTGG
              421 AAAGTCCCGT TGATTTTGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGTGGAGACT
              481 TGGAAATCCC CGTGAGTCAA ACCGCTATCC ACGCCCATTG ATGTACTGCC AAAACCGCAT
              541 CACCATGGTA ATAGCGATGA CTAATACGTA GATGTACTGC CAAGTAGGAA AGTCCCATAA
              601 GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG TCATTGACGT CAATAGGGGG
              661 CGTACTTGGC ATATGATACA CTTGATGTAC TGCCAAGTGG GCAGTTTACC GTAAATACTC
              721 CACCCATTGA CGTCAATGGA AAGTCCCTAT TGNCGTTACT ATGGGAACAT ACGTCATTAT
              781 TGACGTCNAT GNGCGGGGGT CGTTGGGCGG NCAGCCAGGC GGGCNATTTA CNGNAAGTTA
              841 TGTAACGCGG AACTCNNTAT ATGGGCTATG ANCNNANGAN CNCNNNAATT NNNNACTATT
            901 AATAANNANT CNANAATCAA NGTCNNNNNN NATATCNNGN CNNNNNNNNN N
            //
            Primer: RRE_F1 (Pr0796)
            Region: RRE, cPPT_CTS
            Sequence file: RDB17907_B0Aec.seq check
            >04377_17907_B0Ae_1_RRE_F1_D07_10_ABI24.ab1
                1 NNNNNNNNNN NNNCNNCNNN NNNNTGCTGN NGGCTATTGA GGCGCAACAG CATCTGTTGC
               61 AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC
              121 TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG
              181 CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA
              241 CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG
              301 AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG
              361 CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA
              421 TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA
              481 GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC
              541 CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC
              601 GATTAGTGAA CGGATCTACA AATGGCAGTA TTCATCCACA ATTTTAAAAG AAAAGGGGGG
              661 ANTGGGGGGT ACAGTGCAGG GGAAAGAATA GTAGACATAA TAGCAACAGA CATACAAACT
              721 AAAGAATTAC AAAAACNAAT TACAAAAATT CAAAATTTTC GGGTTTATTA CAGGNNCAGC
              781 AGAAATTCAC TTTGAATTAA TTCGATTAAT AGTAATCNAT TACNGGGNCA TTAGTTCATA
              841 GCCNNTATAT GGAGTTNCGC GNTACATAAC NTACNGNAAA TGGCCCGNNN NGNNNANNGC
              901 CCNACNACCC CCGCCCATNN NCNNNNNNNN NGNNNNANNN NCCNNTANNN ACNNNNNNNN
            961 NN
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGHp(A), 3'LTR,
            Sequence file: RDB17907_B0Aed.seq check
            >04377_17907_B0Ae_1_BGH_rev2_E07_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
               61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
              121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
              181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
              241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT
              301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC
              361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC
              421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT
              481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT
              541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCNCTT
              601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC
              661 CTGANGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC
              721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGNTNCN TGTTTTTCTA NGTCTCGANN
              781 GTATCGATGC NGGGNNGCNG CCCNAANGGA NNTNCNANTC GTCTNNNGNC GNANNNNAAN
            841 ANNNNNNNNN N
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori, EM7 pro, Zeo
            Sequence file: RDB17907_B0Aee.seq check
            >04377_17907_B0Ae_1_SV40pro_ori_F_F07_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNGC CTCTGCCTCT GNNCTATTCN NGAAGTAGTG AGGAGGCTTT
               61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT
              121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
              181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
              241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
              301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
              361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
              421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
              481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
              541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
              601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
              661 TCCAGCGCGG GGNTCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
              721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
              781 TGCATTCTAG TTGTGGTTTG TCCAAACTCN TCAANGTATC TTATCATGTC TGTATACNGT
              841 CNNNCTCTAG CTAGANCTTG GCGNAATCNT GGNCATANCT GTTNNCTGNG NGAAANNNNT
            901 ATCCGCNNAC NATNCCNCAC NACNTACNAN NNNNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            reference Niwano, K., Lentiviral vector-mediated SERCA2 gene transfer protects against heart failure and left ventricular remodeling after myocardial infarction in rats. PMID 18388909.
            user_report Luo, W., CLASP2 recognizes tubulins exposed at the microtubule plus-end in a nucleotide state-sensitive manner. Sci. Adv. 9 (1): eabq5404 (2023). PMID 36598991.
            user_report Meshitsuka, S., CRISPR/Cas9 and AAV mediated insertion of β2 microglobulin-HLA-G fusion gene protects mesenchymal stromal cells from allogeneic rejection and potentiates the use for off-the-shelf cell therapy. Regen. Ther. 21: 442-452 (2022). PMID 36313397.
            user_report Ogawa, Y., Endogenously expressed Ranbp2 is not at the axon initial segment. J. Cell Sci. 134 (6): jcs256180 (2021). PMID 33536249.
            user_report Nanba, D., EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis. J. Cell Biol. 220 (11): e202012073 (2021). PMID 34550317.
            user_report Girao, H., CLASP2 binding to curved microtubule tips promotes flux and stabilizes kinetochore attachments. J. Cell Biol. 219 (2). pii: e201905080 (2020). PMID 31757788.
            user_report Lu, F., Downregulation of TMOD1 promotes cell motility and cell proliferation in cervical cancer cells. Oncol. Lett. 19: 3339-3348 (2020). PMID 32218869.
            user_report Kinoshita, N., Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. iScience 15: 28-38 (2019). PMID 31026667.
            user_report Ishikawa, T., Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation. J. Cell. Physiol. 233 (6): 4825-4840 (2018). PMID 29150954.
            user_report Hamada, M., Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR. EBioMedicine 34: 18-26 (2018). PMID 30082227.
            user_report Tanaka, M., Aggregation of scaffolding protein DISC1 dysregulates phosphodiesterase 4 in Huntington's disease. J. Clin. Invest. \127 (4): 1438-1450 (2017). PMID 28263187.
            user_report Ujino, S., Hepatitis C virus utilizes VLDLR as a novel entry pathway. Proc. Natl. Acad. Sci. U.S.A. 113 (1): 188-193 (2016). PMID 26699506.
            user_report Maishi, N., Tumour endothelial cells in high metastatic tumours promote metastasis via epigenetic dysregulation of biglycan. Sci. Rep. 6: 28039 (2016). PMID 27295191.
            user_report Maki, T., CLASP2 Has Two Distinct TOG Domains That Contribute Differently to Microtubule Dynamics. J. Mol. Biol. 427 (14): 2379-2395 (2015). PMID 26003921.
            user_report Watanabe, K., FBXO21 mediates the ubiquitylation and proteasomal degradation of EID1. Genes Cells 20 (8): 667-674 (2015). PMID 26085330.
            user_report Uemura, S., Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells. Glycoconj. J. 31 (2): 101-108 (2014). PMID 23999868.
            user_report Ohnishi, T., Identification and characterization of a neuron-specific isoform of protrudin. Genes Cells 19 (2): 97-111 (2014). PMID 24251978.
            user_report Araya, N., HTLV-1 induces a Th1-like state in CD4+CCR4+ T cells. J. Clin. Invest., 124 (8): 3431-42 (2014). PMID 24960164.
            user_report Grimaldi, A.D., CLASPs are required for proper microtubule localization of end-binding proteins. Dev. Cell 30 (3): 343-352 (2014). PMID 25117684.
            user_report Ishibashi, M., Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. Oncol. Rep. 29 (3): 946-950 (2013). PMID 23292722.
            user_report Takahashi, Y., Paired related homoeobox 1, a new EMT inducer, is involved in metastasis and poor prognosis in colorectal cancer. Br. J. Cancer, 109 (2): 307-311 (2013). PMID 23807160.
            user_report Watanabe, T., FBL2 regulates amyloid precursor protein (APP) metabolism by promoting ubiquitination-dependent APP degradation and inhibition of APP endocytosis. J. Neurosci., 32 (10): 3352-3365 (2012). PMID 22399757.
            user_report Kanemura H., Impaired vascular development in the yolk sac and allantois in mice lacking RA-GEF-1. Biochem. Biophys. Res. Commun., 387, 754-759 (2009). PMID 19635461.
            user_report Sakurai, Y., DNA double strand break repair enzymes function at multiple steps in retroviral infection.
            Retrovirology, 6:114 (2009). PMID 20003485.

            2023.05.22

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