Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*08:01 cDNA

Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB03369
Resource name Human HLA-B*08:01 cDNA
Alternative name HLA-B*0801
Clone info. Human HLA-B*08:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
Other clones in our bank
External Database
Human MHC class I B
          Reference sequence
            
           
          Sequence (full) RDB03369zzt02.seq compiled by us

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03369 Human HLA-B*08:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-B*08:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03369).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19823_B2L6p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB19823_B2L6a.seq check
          >03369_19823_B2L6_2_CMV_Forward_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
             61 NNNNNNNGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTCACCCGG
            181 ACTCAGAATC TCCTCAGACG CCGAGATGCT GGTCATGGCG CCCCGAACCG TCCTCCTGCT
            241 GCTCTCGGCG GCCCTGGCCC TGACCGAGAC CTGGGCCGGC TCCCACTCCA TGAGGTATTT
            301 CGACACCGCC ATGTCCCGGC CCGGCCGCGG GGAGCCCCGC TTCATCTCAG TGGGCTACGT
            361 GGACGACACG CAGTTCGTGA GGTTCGACAG CGACGCCGCG AGTCCGAGAG AGGAGCCGCG
            421 GGCGCCGTGG ATAGAGCAGG AGGGGCCGGA GTATTGGGAC CGGAACACAC AGATCTTCAA
            481 GACCAACACA CAGACTGACC GAGAGAGCCT GCGGAACCTG CGCGGCTACT ACAACCAGAG
            541 CGAGGCCGGG TCTCACACCC TCCAGAGCAT GTACGGCTGC GACGTGGGGC CGGACGGGCG
            601 CCTCCTCCGC GGGCATAACC AGTACGCCTA CGACGGCAAG GATTACATCG CCCTGAACGA
            661 GGACCTGCGC TCCTGGACCG CGGCGGACAC CGCGGCTCAG ATCACCCAGC GCAAGTGGGA
            721 GGCGGCCCGT GTGGCGGANC ANGACAGAGC CTACCTGGAG GGCACGTGCG TGGAGTGGCT
            781 CCGCAGATAC CTGGANAACG GGAAGGANAC GCTGGANCGC GCGGNNCCCC CCAAAGANAC
            841 ACGTGACCCA CCACCCCATC TCTGANCATG AGGCCNCCCN NANGNGCTGG GCCCNGGGCT
            901 TCTACCNTGC NGANNTCACN CTNNNCNNNN NNCGGGNTGN NNNGGNNNNA ANTNNNNNNN
          961 NNN
          //
          Primer: BGH_rev3 (Pr0885)
          Region: insert 3'
          Sequence file: RDB19823_B2L6b.seq check
          >03369_19823_B2L6_2_bGH_rev3_E05_14_ABI24.ab1
              1 NNNNNNNNNN NNNGNNNGGN NGNNGGGCNA ACNACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG NTCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CATCTCAGTC
            121 CCTCACAAGA CAGCTGTCTC AGGCTTTTCA AGCTGTGAGA GACACATCAG AGCCCTGGGC
            181 ACTGTCGCTG CACGCAGCCT GAGAGTAGCT CCCTCCTTTT CCACCTGAGC TCTTCCTCCT
            241 ACACATCACA GCAGCGACCA CAGCTCCGAT GACCACAACT GCTAGGACAG CCAGGCCAGC
            301 AACAATGCCC ACGATGGGGA CGGTGGACTG GGAAGACGGC TCCCATCTCA GGGTGAGGGG
            361 CTTCGGCAGC CCCTCATGCT GTACATGGCA TGTGTATCTC TGCTCTTCTC CAGAAGGCAC
            421 CACCACAGCT GCCCACTTCT GGAAGGTTCT ATCTCCTGCT GGTCTGGTCT CCACAAGCTC
            481 AGTGTCCTGA GTTTGGTCCT CGCCATCCCG CTGCCAGGTC AGTGTGATCT CCGCAGGGTA
            541 GAAGCCCAGG GCCCAGCACC TCAGGGTGGC CTCATGGTCA GAGATGGGGT GGTGGGTCAC
            601 GTGTGTCTTT GGGGGGTCCG CGCGCTCCAG CGTGTCCTTC CCGTTCTCCA GGTATCTGCG
            661 GAGCCACTCC ACGCACGTGC CCTCCAGGTA GGCTCTGTCC TGCTCCGCCA CACGGGCCGC
            721 CTCCCACTTG CGCTGGGTGA TCTGAGCCGC GGTGTCCGCC GCGGTCCANG AGCGCANGTC
            781 CTCGTTCANG GCGATGTAAT CCTTGCCGTC GTANGCGTAC TGGTTATGCC CGCGGNNGAN
            841 GCGCCCGTCC GGCCCCACGT CGCANCCGTA CATGCTCTGG NNGGGTGTGA GANCNNGCCN
            901 CGCNCTNNNN GNAGTANCCG CGNNNNNTNC NCNNNNTCNN NNNGNCANTC TGTGNGTNNN
          961 NNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB19823_B2L6c.seq check
          >03369_19823_B2L6_2_SV40pro_ori_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNC GCCTCTGCCT CTGNNCTATT CNNGAAGTAG TGAGGAGGCT
             61 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT
            121 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            181 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            241 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            301 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            361 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            421 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            481 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            541 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            601 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            661 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGN TCTCGTCGTG ACCCATGGCG
            721 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGNCCGCTT TTCTGGATTC ATCGACTGTG
            781 GCCGGCTGGG TGTGGCGGAN CGCTATCANG NCATANCGTT NGCTACCCGT GATATTGCTG
            841 AAGAGCTTGG CGGCGAATGG GCNGANCGCT TCCTCGNGCT TTACNGNATC GCCGCNCCNN
            901 ANTCGCAGCG NATCGCNNTC NATCGNNNTC NTGNNNNNTT CNTCNGANCG GGNNNNNNGG
          961 NNNNNNNANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl