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pAxCALNLEGFP (reverse)

Shuttle vector for rAd harboring enhanced green fluorescent protein cDNA

Catalog number RDB03260
Resource name pAxCALNLEGFP (reverse)
Alternative name 625C5
Clone info. Enhanced green fluorescent protein (EGFP) is cloned into the SwaI site of pAxCALNLw. (Direction:reverse)
Comment Cre-recombinase is required to remove LNL stuffer sequence. Use COS-TPC method for the production of adenovirus.
Vector backbone pAxCALNLw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 45,995 bp
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Remember that you will be working with samples containing infectious virus.
            提供案内 (日本国内) [open/close]

            提供条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB03260 pAxCALNLEGFP (reverse) Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression regulation (English text)

            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles


            2023.04.10

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