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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMX-N/RBP-J

Expression vector of mouse RBP-J

Catalog number RDB03021
Resource name pCMX-N/RBP-J
Alternative name pCMX-N/RBP-2
Clone info. Expression vector of mouse RBP-J under the control of CMV promoter. Dominant negative form [pCMX-N/RBP-J (R218H), RDB03022] and constitutive active form (pCMX-N/VP16-RBP-J, RDB03023) are available.
Vector backbone pCMX-N (Plasmid)
Size of vector backbone 4,536 bp
Selectable markers Amp^r
Gene/insert name Mouse RBP-J cDNA
Depositor|Developer Honjo, Tasuku | Kuroda, Kazuki |
Other clones in our bank
External Database
Mouse RBP-J
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB03021.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). Acknowledgement to Depositor in publication. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
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          提供条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。研究結果の発表時、謝辞に寄託者名を入れ ること。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB03021 pCMX-N/RBP-J DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMX-N/RBP-J was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03021).

          Reference section:

          Kuroda, K., Tani, S., Tamura, K., Minoguchi, S., Kurooka, H., Honjo, T., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Dnaconda's recommendation EXR0009j (Japanese text)
          Featured content Dnaconda's recommendation EXR0030j (Japanese text)
          Featured content Dnaconda's recommendation EXR0042e (English text)
          Featured content Dnaconda's recommendation EXR0042j (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12301_A3Jm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12301_A3Jma.seq check
          >03021_12301_A3Jm_T7-20bp_C10_07.ab1
              1 CACAATGTCT CTAGAGGATA TCGAATTCCT GCAGCCCAAT ATGCCCTCCG GTTTTCCTCA
             61 GTCTCCACGT ACGTCCCCGA GGGCGCGTCC CAAAACCCGG ATAACCGGAG CGCTCCCCAT
            121 GGACTACTCG GAGGGCTTGT CCGCGGAGGA GCGGCCTGCG CATGCTCCAT CGGCGGGGAA
            181 GTTTGGTGAG CGGCCTCCAC CCAAACGACT CACTAGGGAA GCTATGCGAA ATTATTTAAA
            241 AGAACGAGGG GATCAAACAG TGCTCATTCT TCATGCAAAA GTTGCACAGA AGTCTTACGG
            301 AAATGAAAAA CGATTTTTTT GCCCTCCTCC TTGTGTGTAT CTTATGGGCA GTGGTTGGAA
            361 GAAAAAAAAA GAGCAAATGG AACGAGATGG ATGTTCTGAA CAAGAGTCTC AACCCTGTGC
            421 GTTTATTGGA ATAGGAAACA GTGACCAAGA AATGCAGCAG CTGAACCTCG AGGGGAAGAA
            481 CTACTGCACA GCCAAAACAT TGTACATATC TGATTCAGAC AAGAGAAAAC ATTTCATGTT
            541 GTCTGTAAAG ATGTTCTATG GCAACAGCGA TGACATTGGT GTGTTCCTCA GCAAGCGGAT
            601 AAAGGTCATC TCCAAACCCT CCAAAAAGAA GCAGTCACTG AAGAATGCTG ACTTGTGCAT
            661 TGCTTCAGGA ACGAAGGTGG CACTGTTCAA TCGCCTTCGG TCCCAGACAG TTAGTACCAG
            721 GTACCTGCAT GTAGAAGGAG GGAATTTCCA CGCCAGTTCA CAACAGTGGG GAGCATTTTA
            781 CATCCATCTC TTGGACGACG ACGAGTCGGA AGGAGAGGAG TTCACAGTTA GAGATGGCTA
            841 CATCCATTAC GGGCAGACTG TCAAGCTTGT GTGCTCAGTG ACTGGCATGG CACTCCCAAG
            901 ATTGATAATT AGGAAAGTTG ATAAGCAGAC GGCATTACTG GATGCAGACG ACCCTGTATC
            961 ACAACTCCAC AAATGTGCAT TTTACCTTAA GGATACAGAA AGAATGTACT TGTGCCCTTC
           1021 TCAGAAGATA ATCCCAATTT CAGCCACTCC ATGTCCAAAG ACAAATAAGG AAATGATAAA
           1081 CGATGGAGCT TCCTGGACAT CATAGCAAGG ACCAGCAGAA TACACGTTTC TTATTGAGGA
          1141 ATGGGCCCCG GATCCCTGTG GC
          //
          Primer:
          Sequence file: RDB12301_A3Jmb.seq check
          >03021_12301_A3Jm_pAxEFR2_A01_01.ab1
              1 CGGGAAAAAA TGGCTTTATT TGGTGAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA
             61 TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG
            121 GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT
            181 ATGATCTCTA GTCAAGGCAC TATACATCAA ATATTTTTCC ATAATTTTCT TGTATAGCAG
            241 TGCAGCTTTT TCCTTTGTGG TGTAAATAGC AAAGCAAGCA AGAGTTCTAT TACTAAACAC
            301 AGCATGACTC AAAAAACTTA GCAATTCTGA AGGAAAGTCC TTGGGGTCTT CTACCTTTCT
            361 CTTCTTTTTT GGAGGAGTAG AATGTTGAGA GTCAGCAGTA GCCTCATCAT CACTAGATGG
            421 CATTTCTTCT GAGCAAAACA GGTTTTCCTC ATTAAAGGCA TTCCACCACT GCTCCCATTC
            481 ATCAGTTCCA TAGGTTGGAA TCTAAAATAC ACAAACAATT AGAATCAGTA GTTTAACACA
            541 TTATACACTT AAAAATTTTA TATTTACCTT AGAGCTTTAA ATCTCTGTAG GTAGTTTGTC
            601 CAATTATGTC ACACCACAGA AGTAAGGTTC CTTCACAAAG ATCCTCTAGC TACCTAGCTA
            661 GCTGGCCAGG ATCCCGTCGA CGGATCCCCA ACTTTTTGCT GCATTCAAGT CAGTTTCAGT
            721 CCCAGCAAAA AGACGGTAGT TAGGACACCA CGGTTGCTGT GGACGATGTG ACACTGGTAG
            781 AATTTGTGCT GGCATTTGTG TAATTCCCCT CGCTGTTTGT GTTTGACTCA TTGGAGGGCA
            841 CTTGGCTGGA GTTGGCTCTG AGAATCGCTC CTGCAGCGCT GCAGTGTGGC CTCGGCCCTG
            901 GCTCTGGTGT GTAGGTGAAG GTAAGGCTGG TGGAGTAAAT GACCCCGTCA TTTCGGACCA
            961 AAGTCACTGG AACCTGGACT GGCTGGCGGA CCCATCTCCA ACCCTTCCCG GAATGCAGAA
           1021 ATGTCTGGGA CCACACAGAG CATGCTCTCT CACATCTGTA CATTGTTTCG GCTTCTACAT
           1081 CCCCAACCAC ACTCGTAATT GGAGTAAAGT TTTGTCCTGT AAGTCCAGGC ATGGCCTACG
          1141 TTCCCCCGCC GCCATTCACT GAAAGACTTT TCTTACCGAA CACA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kuroda, K., Delta-induced Notch signaling mediated by RBP-J inhibits MyoD expression and myogenesis. J. Biol. Chem., 274, 7238-7244 (1999). PMID 10066785. [link to RRC of NBRP]
          user_report Sakamoto K., Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms. Lab. Invest., 92 (5): 688-702 (2012). PMID 22330335. [link to RRC of NBRP]
          user_report O'Brien, L.L., Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish. Dev. Biol., 358 (2): 318-330 (2011). PMID 21871448. [link to RRC of NBRP]
          user_report Lee, Y.J., Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells. Stem Cells and Cloning: Advances and Applications, 3: 165-173 (2010). PMID 24198522. [link to RRC of NBRP]
          user_report Sakamoto K., Zfp64 participates in Notch signaling and regulates differentiation in mesenchymal cells. J. Cell Sci., 121, 1613-1623 (2008). PMID 18430783. [link to RRC of NBRP]
          user_report Sakamoto K., Distinct roles of EGF repeats for the Notch signaling system. Exp. Cell Res., 302, 281-291 (2005). PMID 15561108. [link to RRC of NBRP]

          2023.10.05

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