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Rat alpha1-antitrypsin-RVRR mutant

Expression vector of rat alpha1-antitrypsin

Catalog number RDB02941
Resource name Rat alpha1-antitrypsin-RVRR mutant
Clone info. Rat alpha1-antitrypsin RVRR mutant contains Arg-Ile-Arg-Arg in its reactive site. Expression was confirmed by depositor as follows: Immunoprecipitation and Western blotting.
Comment Wild type of rat alpha1-antitrypsin is M32247.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), Neo (Mammalian cell)
Gene/insert name Rat alpha1-antitrypsin cDNA
Depositor|Developer Matsuda, Yoshiko | Tsuji, Akihiko |
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External Database
Rat alpha1-antitrypsin

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Tsuji, A. et al., Protein Eng. 15 (2): 123-130 (2002)) are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって文献 (Tsuji, A. et al., Protein Eng. 15 (2): 123-130 (2002)) の引用を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02941 Rat alpha1-antitrypsin-RVRR mutant Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Tsuji, A., Development of selectivity of alpha1-antitrypsin variant by mutagenesis in its reactive site loop against proprotein convertase. A crucial role of the P4 arginine in PACE4 inhibition. Protein Eng. 15 (2): 123-130 (2002). PMID 11917148.

          2022.05.18

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