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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-C*03:03:01 cDNA

Human HLA-C*03:03:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02884
Resource name Human HLA-C*03:03:01 cDNA
Alternative name HLA-Cw*03031
Clone info. Human HLA-C*03:03:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human HLA-Cw*03031 cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human HLA-Cw*03031
          Reference sequence
            
           
          Sequence RDB02884z.seq
          Remarks, protocol and/or map (gif) RDB02884.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02884 Human HLA-C*03:03:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-C*03:03:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02884).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13722_A5Llp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'
          Sequence file: RDB13722_A5Lld.seq check
          >02884_13722_B1EbS_CMV-Forward_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNGGGAGGNC TATATAAGCA GAGCTCTCTG GCTAACTAGA
             61 GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT
            121 GGCTAGCGTT TAAACTTAAG CTTTTCTCCC CAGACGCCGA GATGCGGGTC ATGGCGCCCC
            181 GAACCCTCAT CCTGCTGCTC TCGGGAGCCC TGGCCCTGAC CGAGACCTGG GCCGGCTCCC
            241 ACTCCATGAG GTATTTCTAC ACCGCTGTGT CCCGGCCCGG CCGCGGGGAG CCCCACTTCA
            301 TCGCAGTGGG CTACGTGGAC GACACGCAGT TCGTGCGGTT CGACAGCGAC GCCGCGAGTC
            361 CGAGAGGGGA GCCGCGGGCG CCGTGGGTGG AGCAGGAGGG GNCGGAGTAT TGGGACCGGN
            421 AGACNCNNAN NNNCNANNNN NNGGCACANN NNGANNGANT GNNNCTGCNN AANCNGCNNN
          481 NNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB13722_A5Lle.seq check
          >02884_13722_B1EbS_bGH_rev3_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNTN NAAGGCACAG TCGAGGCTGA TCAGCGGGTT TAAACGGGCC
             61 CTCTAGACTC GAGCGGCCGC GTCTCAGGCT TTACAAGCGA TGAGAGACTC ATCAGAGCCC
            121 TGGGCACTGT TGCTGGACGC AGCCTGAGAG CAGCTCCCTC CTTTTCCACC TGAGCTCTTC
            181 CTCCTACACA TCACAACAGC CACCACAGCT CCTAGGACAG CTAGGACAGC CAGGACAGCC
            241 AGGCCAGCAA CGATGCCCAC GATGGGGATG GTGGGCTGGG AAGACGGCTC CCATCTCAGG
            301 GTGAGGGGCT CCGGCAGCCC CTCGTGCTGC ACATGGCACG TGTATCTCTG CTCTTCTCCA
            361 GAAGGCACCA CCACAGCTGC CCACTTCTGG AAGGTTCCAT CTCCTGCTGG CCTGGTCTCC
            421 NNNNNNTCAG TGTCCTGAGT TTGGTCCTCC CCATCCCACT GCCNNNNNNG TGTGATCTCC
            481 GCANGGTANA ANCCNNNGNN CCCANNANCN CANGGTGNNN NNNNNGNNNN ANANNNNNAN
          541 NNGNGNGNCN NGTGNGNNNN NNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: Sv40 pro_ori,NeoR
          Sequence file: RDB13722_A5Llf.seq check
          >02884_13722_B1EbS_SV40pro_ori_F_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN ANNCTANTNN NGAANTNNNG AGGAGGCTTT TTTGGAGGCC
             61 TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA
            121 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG
            181 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
            241 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT
            301 CCNGTGCCCT GAATGAACTG CAGGACGAGG CANCGCGGCT ATCGTGGCTG GNNNNNACGG
            361 GCGTTCCTTG NNNNNCNGNN CNCGACGTTG TCNCTGANNC GGGAANGNAC NGNCTGCNNN
          421 TGNNNNNNNN GNNNGGNCNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          2024.03.26

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