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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Human HLA-B*46:01 cDNA

Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02880
Resource name Human HLA-B*46:01 cDNA
Alternative name HLA-B*4601
Clone info. Human HLA-B*46:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MHC class I B cDNA
Depositor|Developer Akatsuka, Yoshiki |
Other clones in our bank
External Database
Human MHC class I B
          Reference sequence
            
           
          Sequence RDB02880z.seq
          Remarks, protocol and/or map (gif) RDB02880.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
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          RDB02880 Human HLA-B*46:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Human HLA-B*46:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02880).

          Reference section:

          Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13718_A5Llp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert 5'
          Sequence file: RDB13718_A5Lld.seq check
          >02880_13718_B0GgF_CMV-Forward_F07_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTNNGNCG GTANNNNNGT ACGGNGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTCACC CGGACTCAGA
            181 ATCTCCTCAG ACGCCGAGAT GCGGGTCACG GCGCCCCGAA CCGTCCTCCT GCTGCTCTCG
            241 GGAGCCCTGG CCCTGACCGA GACCTGGGCC GGCTCCCACT CCATGAGGTA TTTCTACACC
            301 GCCATGTCCC GGCCCGGCCG CGGGGAGCCC CGCTTCATCG CAGTGGGCTA CGTGGACGAC
            361 ACCCAGTTCG TGAGGTTCGA CAGCGACGCC GCGAGTCCGA GGATGGCGCC CCGGGCGCCA
            421 TGGATAGAGC AGGAGGGGCC GGAGTATTGG GACCGGGAGA CACAGAAGTA CAAGCGCCAG
            481 GCACAGACTG ACCGAGTGAG CCTGCGGAAC CTGCGCGGCT ACTACAACCA GAGCGAGGCC
            541 GGGTCTCACA CCCTCCAGAG GATGTACGGC TGCGACGTGG GGCCGGACGG GCGCCTCCTC
            601 CGCGGGCATG ACCAGTCCGC CTACGACGGC AAGGATTACA TCGCCCTGAA CGANGACCTG
            661 AGCTCCTGGA CCGCGGCGGA CACGGCGGCT CAGATCACCC AGCGCAAGTG GGAGGCGGCC
            721 CGTGAGGCGG ANCAGTGGAN ANCCTACCTG GANGGCCTGT GCGNGGANNG GCTCCGCAGA
            781 TACNNGGNNA ACGGGAANGA NANGCTGCAG CGCGCGNNNC CCCCCAAAGA CACATGTGAN
            841 CCNNNACCCC ATCNNNNANN NTGNNNNCNN CCCNNNNNNN CNGGGCCNNN GNCNTNNNNC
          901 CNNNCNGNAN ATNNNNNNNN NN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA, insert 3'
          Sequence file: RDB13718_A5Lle.seq check
          >02880_13718_B0GgF_BGH_rev2_G07_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNG NNNNNNCNAC AGNTGGCTGG CAACTAGAAG GCACAGTCGA
             61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCATCT CAGTCCCTCA
            121 CAAGACAGCT GTCTCAGGCT TTTCAAGCTG TGAGAGACAC ATCAGAGCCC TGGGCACTGT
            181 CGCTGGACGC AGCCTGAGAG TAGCTCCCTC CTTTTCCACC TGAGCTCTTC CTCCTACACA
            241 TCACAGTAGC GACCACAGCT CCGATGACCA CAACTGCTAG GACAGCCAGG CCAGCAACAA
            301 TGCCCACGAT GGGGATGGTG GACTGGGAAG ATGGCTCCCA TCTCAGGGTG AGGGGCTTCG
            361 GCAGCCCCTC ATGCTGTACA TGGCATGTGT ATCTCTGCTC TTCTCCAGAA GGCACCACCA
            421 CAGCTGCCCA CTTCTGGAAG GTTCTATCTC CTGCTGGTCT GGTCTCCACA AGCTCGGTGT
            481 CCTGAGTTTG GTCCTCGCCA TCCCGCTGCC AGGTCAGTGT GATCTCCGCA GGGTAGAAGC
            541 CCAGGGCCCA GCACCTCAGG GTGGCCTCAT GGTCAGAGAT GGGGTGGTGG GTCACATGTG
            601 TCTTTGGGGG GTCCGCGCGC TGCAGCGTCT CCTTCCCGTT CTCCAGGTAT CTGCGGANCC
            661 ACTCCACGCA CANGCCCTCC NGGTANGCTC TCCACTGCTC CGCCTCACGG GCCGCCTCCC
            721 ACTTGCGCTG GGTGATCTGA NCCGCCGNGT CCGCCGCGGT CCAGGANCTC NNNCCTCNTT
            781 CAGGGCGATG TAATCCTTGN CGTCNNANGC GGACTGNTCA TGNCCGCGGA NGANNNGCCN
            841 NNNCNNNCCC ACNTCGNANC CGNACNTNCT CNGGNNGGNN NNANANCNNG NCNNCNNTNN
          901 NGNNNNANTA NNNNNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40pro_ori, NeoR
          Sequence file: RDB13718_A5Llf.seq check
          >02880_13718_B0GgF_SV40pro_ori_F_H07_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCNGC CTCTGANCTA NTCNNGAAGT AGTGAGGAGG CTTTTTTGGA
             61 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA
            121 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG
            181 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT
            241 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC
            301 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG
            361 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG
            421 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA
            481 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA
            541 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT
            601 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC
            661 AGGCTCAAGG CGCGCATGCC CGACGGCGAN GATCTCGTCG NGACCCATGG CGATGCCNGC
            721 TTGCCGAATA TCATGGTGGA AAATGGNCGC TTTTCTGGAN TCNTCGACNG NGGNCGGCTG
            781 GGNGTGGCGG ANCGCTATCN NNACNTANNG TTGGCTANCC GNGATATTGC TGAAGANCTT
            841 GGNNGNCNAA NGGGCNNANN NNTTCCNNNN GCTTTACGGN NTCNNNNNNN CNNANTNNNA
          901 NNNNATCNNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

          2023.08.18

          GNP_filter3_RDBDEP_html_230702.pl